A three-dimensional electron spin resonance microscope

Abstract

Articles you may be interested inHigh-sensitivity Q-band electron spin resonance imaging system with submicron resolution Rev. Sci. Instrum. 82, 043708 (2011);A broadband pulsed radio frequency electron paramagnetic resonance spectrometer for biological applications Rev.An electron spin resonance (ESR) imaging system, capable of acquiring three-dimensional (3D) images with a resolution of ϳ10ϫ 10ϫ 30 m in a few minutes of acquisition, is presented. This ESR microscope employs a commercial continuous wave ESR spectrometer, working at 9.1 GHz, in conjunction with a miniature imaging probe (resonator+ gradient coils), gradient current drivers, and control software. The system can acquire the image of a small ͑ϳ1.5ϫ 1.5ϫ 0.25 mm͒ sample either by the modulated field gradient method, the projection reconstruction method, or by a combination of the two. A short discussion regarding the resolution of the modulated field gradient method in two-dimensional (2D) and 3D imaging is given. Detailed descriptions of the various system components are provided, along with several examples of 2D and 3D images that demonstrate the capabilities of the system.

ESR micro-imaging of LiNc-BuO crystals in PDMS: Spatial and spectral grain distribution

“…1A. The system is in principle based on the one developed at Cornell University [5,11], with some major changes and upgrades. These mainly include: (1) The use of a wideband ''home-made" microwave bridge rather than a narrowband commercial system; (2) Imaging probe with improved heat dissipation properties; (3) Advanced data acquisition and processing software; and (4) Improved sample holders that enable the positioning of the sample in the center of the resonator.…”

“…The system supports the acquisition of 1-, 2-, 3-, or 4-D spatial and spectral-spatial ESR images via both the projection reconstruction and the modulated fields gradient methods [12]. The magnetic field is swept by the local gradient coils located in the imaging probe that also provide the modulation and field-frequency lock functionality to the system [11]. A frequency counter/power meter (item m in Fig.…”

Photolithographic production of glass sample holders for improved sensitivity and resolution in ESR microscopy

“…ESR microscopy (ESRM) is complementary to the more common in vivo imaging approach. It is based on scaling down the size of the imaging probe employed and thereby measuring small microscopic samples by the use of miniature resonators and gradient coils [1,4,5]. The scaling down, along with the use of relatively high microwave frequencies (10-60 GHz vs. about 0.2-1 GHz employed in in vivo ESR) enables one to significantly improve upon both spin sensitivity and image resolution (down to about 1 ~tm resolution).…”

“…We have found it most useful to construct the resonator from a single crystal of high-permittivity material (e.g., SrTiO 3,or TiO2) and to produce it in the shape of a ring [1,4,16,17]. The compactness of the resonator increases the density of the etectromagnetic energy stored at the sample position.…”

Free Radicals in Living Systems: In Vivo Detection of Bioradicals with EPR Spectroscopy

“…This requires a strong field gradient for EPR imaging, which is 100-1000 times stronger than that for MRI. An EPR microscope with 10 µm image resolution within a few minutes of acquisition has recently been reported by Blank et al [78]. Their microscope is based on an X-band CW-EPR spectrometer.…”