A Three-Dimensional Lung Cell Model to Leptospira Virulence Investigations

Campos, Camila L.; Gomes, Luciana Rodrigues; Covarrubias, Ambart E.; Kato, Ellen Emi; Souza, Gisele G.; Vasconcellos, Sílvio Arruda; Heinemann, Marcos Bryan; Martins, Elizabeth A. L.; Ho, Paulo Lee; Costa, Ana Rita; Silva, Josefa Bezerra da

doi:10.1007/s00284-021-02720-5

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…Campos et al demonstrated a three-dimensional human cell lung spheroids model for Leptospira infection that was efficiently able to infiltrate at the core of cell aggregates and proposed a standardization of inoculation for infection. The study also evaluated virulence and motility through assessment of gene expression and production of chemokines (Campos et al 2022 ).…”

Section: Three-dimensionsal Cell Culture Methodsmentioning

confidence: 99%

Harnessing three-dimensional (3D) cell culture models for pulmonary infections: State of the art and future directions

Shah,

Raghani,

Chorawala

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Pulmonary infections have been a leading etiology of morbidity and mortality worldwide. Upper and lower respiratory tract infections have multifactorial causes, which include bacterial, viral, and rarely, fungal infections. Moreover, the recent emergence of SARS-CoV-2 has created havoc and imposes a huge healthcare burden. Drug and vaccine development against these pulmonary pathogens like respiratory syncytial virus, SARS-CoV-2, Mycobacteria , etc., requires a systematic set of tools for research and investigation. Currently, in vitro 2D cell culture models are widely used to emulate the in vivo physiologic environment. Although this approach holds a reasonable promise over pre-clinical animal models, it lacks the much-needed correlation to the in vivo tissue architecture, cellular organization, cell-to-cell interactions, downstream processes, and the biomechanical milieu. In view of these inadequacies, 3D cell culture models have recently acquired interest. Mammalian embryonic and induced pluripotent stem cells may display their remarkable self-organizing abilities in 3D culture, and the resulting organoids replicate important structural and functional characteristics of organs such the kidney, lung, gut, brain, and retina. 3D models range from scaffold-free systems to scaffold-based and hybrid models as well. Upsurge in organs-on-chip models for pulmonary conditions has anticipated encouraging results. Complexity and dexterity of developing 3D culture models and the lack of standardized working procedures are a few of the setbacks, which are expected to be overcome in the coming times. Herein, we have elaborated the significance and types of 3D cell culture models for scrutinizing pulmonary infections, along with the in vitro techniques, their applications, and additional systems under investigation. Graphical Abstract

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Section: Three-dimensionsal Cell Culture Methodsmentioning

confidence: 99%

Harnessing three-dimensional (3D) cell culture models for pulmonary infections: State of the art and future directions

Shah,

Raghani,

Chorawala

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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“…[2][3][4] In addition, due to the price and ethical limitations, some susceptible species do not fully represent human infection, or sometimes do not develop the expected symptoms. [5,6] Carefully conserved tissues pretreated by different tissue fixation techniques, such as formalin and Hepes-Glutamic acid buffer mediated Organic solvent Protection Effect based techniques, have been widely applied for immunohistochemical studies to characterize the immunological profiles of lung infection. [7,8] These data provide limited information on the molecular events involved in human lung infection in vivo, owing to the unexpected effects of fixatives on tissues.…”

Section: Introductionmentioning

confidence: 99%

Short-term ex vivo tissue culture models help study human lung infectionsA review

Xia

Zeng

et al. 2023

Medicine

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Most studies on human lung infection have been performed using animal models, formalin or other fixed tissues, and in vitro cultures of established cell lines. However, the experimental data and results obtained from these studies may not completely represent the complicated molecular events that take place in intact human lung tissue in vivo. The newly developed ex vivo short-term tissue culture model can mimic the in vivo microenvironment of humans and allow investigations of different cell types that closely interact with each other in intact human lung tissues. Therefore, this kind of model may be a promising tool for future studies of different human lung infections, owing to its special advantages in providing more realistic events that occur in vivo. In this review, we have summarized the preliminary applications of this novel short-term ex vivo tissue culture model, with a particular emphasis on its applications in some common human lung infections. Abbreviations: AEC = alveolar epithelial cell, AM = alveolar macrophage, C. pneumoniae = Chlamydia pneumoniae, CD163 = cluster of differentiation 163, COPD = chronic obstructive pulmonary disease, COVID-19 = coronavirus disease 2019, CXCL = C-X-C motif chemokine ligand, EVLP = ex vivo lung perfusions, H. influenzae = Haemophilus influenzae, IFNs = interferons, IL = interleukin, L. pneumophila = Legionella pneumophila, MAPK = mitogen-activated protein kinase, NTHi = nontypeable Haemophilus influenzae, pHp = pulmonary haptoglobin, S. pneumoniae = Streptococcus pneumoniae, SARS-CoV-2 = severe acute respiratory syndrome coronavirus 2, TLR2 = Toll-like receptor 2, TLR4 = Toll-like receptor 4, TNF-α = tumor necrosis factor-α.

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A Three-Dimensional Lung Cell Model to Leptospira Virulence Investigations

Cited by 2 publications

References 37 publications

Harnessing three-dimensional (3D) cell culture models for pulmonary infections: State of the art and future directions

Harnessing three-dimensional (3D) cell culture models for pulmonary infections: State of the art and future directions

Short-term ex vivo tissue culture models help study human lung infectionsA review

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