A Three-Dimensional Polycaprolactone Scaffold Combined with a Drug Delivery System Consisting of Electrospun Nanofibers

Yoon, Hun‐Young; Kim, Geunhyung

doi:10.1002/jps.22310

Cited by 55 publications

(13 citation statements)

References 38 publications

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“…Many other studies have also shown that ECM-like nanofibers with tailored nanofiber architecture (diameter, aligned or random orientation, pore size, porosity), controlled degradability, and immobilized soluble signal can provide desired cell stimulatory cues to promote cell adhesion, orientation, proliferation, differentiation, and tissue formation [5,6]. Exploiting these advantages, electrospun nanofibers have been tested for engineering bone tissue [7,8], wound dressing [9,10], artificial vessel formation [11], neural tissue regeneration [12,13], and vehicles for drug delivery [14,15] and biomolecular transport [16]. …”

Section: Introductionmentioning

confidence: 99%

Focal adhesion kinase regulation in stem cell alignment and spreading on nanofibers

Andalib

Lee

et al. 2016

Biochemical and Biophysical Research Communications

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While electrospun nanofibers have demonstrated the potential for novel tissue engineering scaffolds, very little is known about the molecular mechanism of how cells sense and adapt to nanofibers. Here, we revealed the role of focal adhesion kinase (FAK), one of the key molecular sensors in the focal adhesion complex, in regulating mesenchymal stem cell (MSC) shaping on nanofibers. We produced uniaxially aligned and randomly distributed nanofibers from poly(L-lactic acid) to have the same diameters (about 130 nm) and evaluated MSC behavior on these nanofibers comparing with that on flat PLLA control. C3H10T1/2 murine MSCs exhibited upregulations in FAK expression and phosphorylation (pY397) on nanofibrous cultures as assessed by immunoblotting, and this trend was even greater on aligned nanofibers. MSCs showed significantly elongated and well-spread morphologies on aligned and random nanofibers, respectively. In the presence of FAK silencing via small hairpin RNA (shRNA), cell elongation length in the aligned nanofiber direction (cell major axis length) was significantly decreased, while cells still showed preferred orientation along the aligned nanofibers. On random nanofibers, MSCs with FAK-shRNA showed impaired cell spreading resulting in smaller cell area and higher circularity. Our study provides new data on how MSCs shape their morphologies on aligned and random nanofibrous cultures potentially via FAK-mediated mechanism.

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Section: Introductionmentioning

confidence: 99%

Focal adhesion kinase regulation in stem cell alignment and spreading on nanofibers

Andalib

Lee

et al. 2016

Biochemical and Biophysical Research Communications

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“…The 15-mer peptide was encapsulated in the PEO layer with variation in the thickness of the PCL layers [48]. They continue their work on PCL and PEO monitoring the release of Rhodamine B [50], earlier this year reporting ways to overcome edge-effects in layer-bylayer electrospun micro/nanofibrous mats of PCL and PEO with the ultimate goals of soft and hard tissue regeneration [51].…”

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confidence: 99%

Electrospun matrices for localised controlled drug delivery: release of tetracycline hydrochloride from layers of polycaprolactone and poly(ethylene-co-vinyl acetate)

Alhusein

Blagbrough

Bank

2012

Drug Deliv. and Transl. Res.

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We report the controlled release of tetracycline (Tet) HCl from a three-layered electrospun matrix for the first time. Five formulations of electrospun poly-ε-caprolactone (PCL) and poly(ethylene-co-vinyl acetate) (PEVA) have been designed, prepared as micro/nanofibre layers, and assayed for the controlled release of the clinically useful antibiotic Tet HCl with potential applications in wound healing and especially in complicated skin and skin-structure infections. Tet HCl was also chosen as a model drug possessing a good ultraviolet (UV) chromophore and capable of fluorescence together with limited stability. Tet HCl was successfully incorporated (essentially quantitatively at 3 %, w/w) and provided controlled release from multilayered electrospun matrices. The Tet HCl release test was carried out by a total immersion method on 2 × 2 cm(2) electrospun fibrous mats in Tris or phosphate-buffered saline heated to 37 °C. The formulation PCL/PEVA/PCL with Tet HCl in each layer gave a large initial (burst) release followed by a sustained release. Adding a third layer to the two-layered formulations led to release being sustained from 6 days to more than 15 days. There was no detectable loss of Tet chemical stability (as shown by UV and NMR) or bioactivity (as shown by a modified Kirby-Bauer disc assay). Using Tet HCl-sensitive bacteria, Staphylococcus aureus (ATCC 25923), the Tet HCl-loaded three-layered matrix formulations were still showing significantly higher antibacterial effects on days 4 and 5 than commercially available Antimicrobial Susceptibility Test Discs of Tet HCl. Electrospinning provides good encapsulation efficiency of Tet HCl within PCL/PEVA/PCL polymers in micro/nanofibre layers which display sustained antibiotic release.

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“…Using the core-shell spinning technique, matrices can also be used as drug delivery systems (DDS) in order to release incorporated drugs in a controlled manner. Yoon et al used a core-shell laminated, structured, electrospun mat of hydrophobic PCL and hydrophilic poly(ethylene oxide) (PEO)/rhodamine-B fibers in a normal PCL matrix [28] . Rhodamine was released from the scaffold by the drug delivery system, which was controlled by the thickness of the PCL layer.…”

Section: Muscle Tissue Engineeringmentioning

confidence: 99%

Nanotechnologies in tissue engineering

Bigdeli

Lyer

Detsch

et al. 2013

Nanotechnology Reviews

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As an interdisciplinary field, tissue engineering (TE) aims to regenerate tissues by combining the principles of cell biology, material science, and biomedical engineering. Nanotechnology creates new materials that might enable further tissue-engineering applications. In this context, the introduction of nanotechnology and nanomaterials promises a biomimetic approach by mimicking nature. This review summarizes the current scope of nanotechnology implementation possibilities in the field of tissue engineering of bone, muscle, and vascular grafts with forms on nanofibrous structures.

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A Three-Dimensional Polycaprolactone Scaffold Combined with a Drug Delivery System Consisting of Electrospun Nanofibers

Cited by 55 publications

References 38 publications

Focal adhesion kinase regulation in stem cell alignment and spreading on nanofibers

Focal adhesion kinase regulation in stem cell alignment and spreading on nanofibers

Electrospun matrices for localised controlled drug delivery: release of tetracycline hydrochloride from layers of polycaprolactone and poly(ethylene-co-vinyl acetate)

Nanotechnologies in tissue engineering

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