A Three-Dimensional View of Precursor Messenger RNA Metabolism Within the Mammalian Nucleus

Carter, Kenneth C.; Bowman, Douglas S.; Carrington, Walter A.; Fogarty, Kevin E.; McNeil, John A.; Fay, Fredric S.; Lawrence, Jeanne B.

doi:10.1126/science.8446902

Cited by 342 publications

(235 citation statements)

References 38 publications

Supporting

Mentioning

219

Contrasting

Order By: Relevance

“…Newly synthesized heteronuclear RNA and small nuclear RNA are enriched on the nuclear matrix [Huang and Spector, 19911. The nuclear matrix has also been shown to be the site of attachment for products from RNA cleavage and for RNA processing intermediates [Carter et al, 1993;Xing et al, 19931. Spliceosome complexes involved in the regulation of RNA splicing have been localized to the nuclear matrix.…”

Section: N a Organization A N D The Nuclear Matrixmentioning

confidence: 99%

Nuclear matrix and the regulation of gene expression: Tissue specificity

Getzenberg

1994

J of Cellular Biochemistry

135

View full text Add to dashboard Cite

Tissue specific regulation of gene expression by a single transcription factor or group of transcription factors cannot be explained simply by DNA sequence alone. For example, in the same animal a particular transcription factor is capable of interacting with DNA in the nucleus of many different cell types, resulting in unique gene expressions despite the presence of a similar genome in all cells. Historically, these differences in response to a single type of factor within target tissues in the same animal have been suggested to occur through different alterations in chromatin structure. Recent, data has demonstrated that combinations of hormones and transcription factors working together may cooperatively play a role in the regulation of gene expression [Pearce and Yamamoto (1 993): Science 259:1161-I 1651. However, the molecular mechanisms of this tissue specific regulation of gene expression still remains largely unexplained. Current evidence suggests that in different cell types the interplay between the specific threedimensional organization of the genome and the structural components of the nucleus, the nuclear matrix, may accomplish the regulation of specific gene expression.

show abstract

Section: N a Organization A N D The Nuclear Matrixmentioning

confidence: 99%

Nuclear matrix and the regulation of gene expression: Tissue specificity

Getzenberg

1994

J of Cellular Biochemistry

135

View full text Add to dashboard Cite

show abstract

“…It was the observation that the nuclear matrix is composed of a broad spectrum of cell-type and tissue-specific proteins, as well as hnRNAs [Fey and Penman, 1988;Nickerson et al, 1990;Getzenberg and Coffey, 1990a;Fey et al, 1984a;Nickerson and Penman, 1992;Berezney, 1991;Berezney and Coffey, 1975;Fey et al, 1984b;Feyet al, 1988;Penman, 1991;Getzenberg et al, 1991a,b;Wan et al, 19941, which provided compelling evidence for nuclear matrix participation in the regulation of gene expression. Further indications for contributions of the nuclear matrix to regulation of gene expression includes sites for DNA replication Berezney, 1991;Berezney and Coffey, 1975;Hozak et al, 1993;Pienta et al, 19911, preferential association with actively transcribed genes [Nelkin et al, 1980;Robinson et al, 1982;Schaack et al, 1990;Stief et al, 1989;Zenk et al, 1990;Cockerill and Garrard, 1986;Dworetzky et al, 1992;Gasser and Laemmli, 1986;Ward and Coffey, 19901, RNA synthesis at fixed transcriptional complexes and pre-mRNA splicing Lawrence et al, 1989;Zeitlin et al, 1987;Carter et al, 1993;Xing and Lawrence, 1991;Spector et al, 1991;Spector, 1990;Xing et al, 1993;Fey et al, 1986;Penman, 19911, as well as specific association of some but not all steroid hormone receptors [Barrack and Coffey, 1983;…”

Section: Introductionmentioning

confidence: 99%

Nuclear architecture supports integration of physiological regulatory signals for transcription of cell growth and tissue‐specific genes during osteoblast differentiation

Stein

Wijnen

Lian

et al. 1994

J of Cellular Biochemistry

View full text Add to dashboard Cite

During the past several years it has become increasingly evident that the three-dimensional organization of the nucleus plays a critical role in transcriptional control. The principal theme of this prospect will be the contribution of nuclear structure to the regulation of gene expression as functionally related to development and maintenance of the osteoblast phenotype during establishment of bone tissue-like organization. The contributions of nuclear structure as it regulates and i s regulated by the progressive developmental expression of cell growth and bone cell related genes will be examined. We will consider signalling mechanisms that integrate the complex and interdependent responsiveness to physiological mediators of osteoblast proliferation and differentiation. The focus will be on the involvement of the nuclear matrix, chromatin structure, and nucleosome organization in transcriptional control of cell growth and bone cell related genes. Findings are presented which are consistent with involvement of nuclear structure in gene regulatory mechanisms which support osteoblast differentiation by addressing four principal questions: 1 ) Does the representation of nuclear matrix proteins reflect the developmental stage-specific requirements for modifications in transcription during osteoblast differentiation? 2 ) Are developmental stage-specific transcription factors components of nuclear matrix proteins? 3) Can the nuclear matrix facilitate interrelationships between physiological regulatory signals that control transcription and the integration of activities of multiple promoter regulatory elements? 4) Are alterations in gene expression and cell phenotypic properties in transformed osteoblasts and osteosarcoma cells reflected by modifications in nuclear matrix proteins? There is a striking representation of nuclear matrix proteins unique to cells, tissues as well as developmental stages of differentiation, and tissue organization. Together with selective association of regulatory molecules with the nuclear matrix in a growth and differentiation-specific manner, there is a potential for application of nuclear matrix proteins in tumor diagnosis, assessment of tumor progression, and prognosis of therapies where properties of the transformed state of cells is modified. It i s realistic to consider the utilization of nuclear matrix proteins for targeting regions of cell nuclei and specific genomic domains on the basis of developmental phenotypic properties or tissue pathology. z 1994 WlIey-Llss, Inc

show abstract

“…This probe also labeled the same late phase clusters that contain snRNP (compare g and h). Oligo dT probes have been previously shown to label the splicing factor rich interchromatin granule network in uninfected cells (Carter et al, 1991(Carter et al, , 1993Visa et al, 1993, see also Fig. 4 h).…”

Section: Spliced Viral Rnas and Polyadenylated Rnas Are Present In Lamentioning

confidence: 99%

“…Perichromatin fibrils are thought to be the in situ forms of newly synthesized hnRNA. Interchromatin granules are not strongly labeled by a pulse of [3H]uridine and are therefore not likely to be locations of transcription, but several studies have shown that polyadenylated RNA is present in these structures (Carter et al, 1991(Carter et al, , 1993Visa et al, 1993). SnRNP and several nonsnRNP splicing factors accumulate at both perichromatin fibrils and interchromatin granules, raising the possibility that splicing of precursor RNA may occur at these locations.…”

mentioning

confidence: 99%

Spliced exons of adenovirus late RNAs colocalize with snRNP in a specific nuclear domain.

Bridge

Riedel

Johansson

et al. 1996

The Journal of Cell Biology

View full text Add to dashboard Cite

Abstract. Posttranscriptional steps in the production of mRNA include well characterized polyadenylation and splicing reactions, but it is also necessary to understand how RNA is transported within the nucleus from the site of its transcription to the nuclear pore, where it is translocated to the cytoplasmic compartment. Determining the localization of RNA within the nucleus is an important aspect of understanding RNA production and may provide clues for investigating the trafficking of RNA within the nucleus and the mechanism for its export to the cytoplasm. We have previously shown that late phase adenovirus-infected cells contain large clusters of snRNP and non-snRNP splicing factors; the presence of these structures is correlated with high levels of viral late gene transcription. The snRNP clusters correspond to enlarged interchromatin granules present in late phase infected cells. Here we show that polyadenylated RNA and spliced tripartite leader exons from the viral major late transcription unit are present in these same late phase snRNP-containing structures. We find that the majority of the steady state viral RNA present in the nucleus is spliced at the tripartite leader exons. Tripartite leader exons are efficiently exported from the nucleus at a time when we detect their accumulation in interchromatin granule clusters. Since the enlarged interchromatin granules contain spliced and polyadenylated RNA, we suggest that viral RNA may accumulate in this late phase structure during an intranuclear step in RNA transport.T HE production of eukaryotic mRNAs can be considered in several steps; these include transcription, polyadenylation, splicing, and export of mRNA from the nucleus to the cytoplasm. Export involves movement of the RNA within the nucleus from the site of transcription to the nuclear pore, followed by transport of the message through the nuclear pore and into the cytoplasm. Although it is convenient to think of these posttranscriptional processes as separate steps, they are likely to be inter-related during the production of RNA in vivo. All of these reactions occur within the structural framework of the nucleus; thus the nuclear organization of gene expression activities has recently received considerable attention (for reviews see Carter, 1994;Wansink et al., 1994;.The nucleus has a high degree of organization with the nucleolus as the most obvious and well characterized nuclear compartment (Scheer et al., 1993 Fakan, 1994). These include perichromatin fibrils, interchromatin granules, and coiled bodies. Perichromatin fibrils are strongly labeled after short pulses of [3H]uridine indicating that transcription occurs at these structures. Perichromatin fibrils are thought to be the in situ forms of newly synthesized hnRNA. Interchromatin granules are not strongly labeled by a pulse of [3H]uridine and are therefore not likely to be locations of transcription, but several studies have shown that polyadenylated RNA is present in these structures (Carter et al., 1991(Carter et al., , 1993Visa et al....

show abstract

A Three-Dimensional View of Precursor Messenger RNA Metabolism Within the Mammalian Nucleus

Cited by 342 publications

References 38 publications

Nuclear matrix and the regulation of gene expression: Tissue specificity

Nuclear matrix and the regulation of gene expression: Tissue specificity

Nuclear architecture supports integration of physiological regulatory signals for transcription of cell growth and tissue‐specific genes during osteoblast differentiation

Spliced exons of adenovirus late RNAs colocalize with snRNP in a specific nuclear domain.

Contact Info

Product

Resources

About