A three-piece, all-glass system for radial chromatography

Rosler, Karl-Heinz A.; Blomster, Ralph N.; Schocken, Mark J.

doi:10.1021/ed063p813

J. Chem. Educ.

1986

DOI: 10.1021/ed063p813

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A three-piece, all-glass system for radial chromatography

Karl-Heinz A. Rosler¹,

Ralph N. Blomster²,

Mark J. Schocken³

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2011

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“…Radial chromatography (also known as centrifugal thin-layer chromatography) has found some, but not widespread, application in organic chemistry laboratory curricula. − The technique offers several advantages over traditional column chromatography including ease of operation, low solvent consumption, good reproducibility, high separation efficiency, and a reusable stationary phase (silica plates can be used multiple times). In addition, the technique is automated and allows for rapid separations when compared to traditional column experiments.…”

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Radial Chromatography for the Separation of Nitroaniline Isomers

Miller

Case

2011

J. Chem. Educ.

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Separation techniques are usually presented in the undergraduate organic laboratory to teach students how to purify and isolate compounds. Often the concept of liquid chromatography is introduced by having students create “silica gel columns” to separate components of a reaction mixture. Although useful, column chromatography can be a laborious technique for students to perform. The separation efficiency can be compromised if students do not prepare the column correctly, and the time required to separate substances can extend beyond a typical laboratory period. Herein, the use of radial chromatography (centrifugal thin-layer chromatography) as an alternative to the traditional column chromatography technique utilized in many teaching laboratories is discussed. Radial chromatography can be used to introduce the principles that underlie liquid chromatography, with the added benefit of providing a relatively fast and highly efficient separation. An experiment for the efficient separation of a mixture containing 2-nitroaniline and 3-nitroanilne using radial chromatography is presented. Typical student results show that each substance can be collected with a high percent recovery (∼70–80%). Furthermore, the melting range of each isolated substance is found to be in agreement with literature values. The separation can be completed in 40–50 min once the sample has been loaded.

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Radial Chromatography for the Separation of Nitroaniline Isomers

Miller

Case

2011

J. Chem. Educ.

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Inactivation of enveloped viruses by anthraquinones extracted from plants

Sydiskis

Owen

Lohr

et al. 1991

Antimicrob Agents Chemother

227

156

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To determine the extent of antiviral activity present in a number of plant extracts, hot glycerin extracts were prepared from Rheum officinale, Aloe barbadensis, Rhamnus frangula, Rhamnus purshianus, and Cassia angustifolia and their virucidal effects were tested against herpes simplex virus type 1. All the plant extracts inactivated the virus. The active components in these plants were separated by thin-layer chromatography and identified as anthraquinones. A purified sample of aloe emodin was prepared from aloin, and its effects on the infectivity of herpes simplex virus type 1 and type 2, varicella-zoster virus, pseudorabies virus, influenza virus, adenovirus, and rhinovirus were tested by mixing virus with dilutions of aloe emodin for 15 min at 37°C, immediately diluting the sample, and assaying the amount of infectious virus remaining in the sample. The results showed that aloe emodin inactivated all of the viruses tested except adenovirus and rhinovirus. Electron microscopic examination of anthraquinone-treated herpes simplex virus demonstrated that the envelopes were partially disrupted. These results show that anthraquinones extracted from a variety of plants are directly virucidal to enveloped viruses.

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A three-piece, all-glass system for radial chromatography

Cited by 2 publications

References 0 publications

Radial Chromatography for the Separation of Nitroaniline Isomers

Radial Chromatography for the Separation of Nitroaniline Isomers

Inactivation of enveloped viruses by anthraquinones extracted from plants

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