A three‐step allele‐level DRB1‐DRB3‐DRB4‐DRB5 genotyping assay using polymerase chain reaction with immobilized sequence‐specific oligoprobes

Buyse, Inge M.; Couture, Carole; Sargent, Michaelg .; Hashemi-Tavoularis, S.

doi:10.1111/j.1399-0039.1997.tb02873.x

Cited by 13 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HLA-DR generic level typing was performed by reverse sequence specific oligonucleotide (SSO) method using the Amplicor HLA-DRB test (Roche Diagnostic System, Branchburg, NJ, USA), and then DRB1 genotyping was done using the polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) method as previously described [15,16]. HLA-DRB3 genotyping was performed using group-specific PCR for exon 2 of DRB3 [17] and SSCP method [18] as previously described.…”

Section: Methodsmentioning

confidence: 99%

HLA-DRB1 and -DRB3 allele frequencies and haplotypic associations in Koreans

et al. 2004

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

HLA-DRB1 and -DRB3 allele frequencies and haplotypic associations in Koreans

et al. 2004

View full text Add to dashboard Cite

“…DRB1 and DRB5 genes were amplified using group‐specific primers and SSCP analysis was performed according to the methods described previously (7, 8) with minor modifications. HLA‐DRB3 PCR (9) and SSCP (10) were performed as previously described. The HLA‐DRB4 gene was amplified for exons 2 and 3 (11) and restriction fragment length polymorphism (RFLP) analysis was performed to determine DRB4*0101, *0102 and *0103 (12).…”

Section: Methodsmentioning

confidence: 99%

HLA class II allele and haplotype frequencies in Koreans based on 107 families

et al. 2002

View full text Add to dashboard Cite

We have investigated the distribution of HLA class II alleles and haplotypes in 107 Korean families (207 parents and 291 children) for the HLA-DRB1, DRB3/B4/B5, DQA1, DQB1 and DPB1 loci. Numbers of alleles observed for each locus were DRB1: 25, DQA1: 14, DQB1: 15, and DPB1: 13. Only two to three alleles were observed for the DRB3 (*0101, *0202, *0301), DRB4 (*0103, * 0103102 N), and DRB5 (*0101, *0102) loci. These alleles showed strong associations with DRB1 alleles: DRB3*0101 with DRB1*1201, *1301 and *1403; DRB3*0301 with DRB1*1202 and *1302; DRB3*0202 with DRB1*0301, *1101, *1401 and *1405; DRB5*0101 and *0102 were exclusively associated with DRB1*1501 and *1502, respectively. The seven most common DRB1-DQB1 haplotypes of frequencies > 0.06 accounted for 52% of the total haplotypes. These haplotypes were exclusively related with the seven most common DRB1-DRB3/B4/B5-DQA1-DQB1 haplotypes: DRB1*1501-DRB5*0101-DQA1*0102-DQB1*0602 (0.085), DRB1*0405-DRB4*0103-DQA1*0303-DQB1*0401 (0.082), DRB1*09012-DRB4*0103-DQA1*0302-DQB1*03032 (0.082), DRB1*0101-DQA1*0101-DQB1*0501 (0.075), DRB1*0701-DRB4*0103-DQA1*0201-DQB1*0202 (0.065), DRB1*0803-DQA1*0103-DQB1*0601 (0.065), and DRB1*1302-DRB3*0301-DQA1*0102-DQB1*0604 (0.065). When these haplotypes were extended to the DPB1 locus, much diversification of haplotypes was observed and only one haplotype remained with a frequency of > 0.06: DRB1*0405-DRB4*0103-DQA1*0303-DQB1*0401-DPB1*0501 (0.062). Such diversification would have resulted from cumulated events of recombination within the HLA class II region, and the actual recombination rate observed between the HLA-DQB1 and DPB1 loci was 2.3% (10/438 informative meioses, including 2 recombinants informative by analysis of TAP genes). Comparison of the distribution of DRB1-DQB1 haplotypes with other populations revealed that Koreans are closest to Japanese people. However, Koreans share a few haplotypes with white people and Africans, which are rare in Japanese: DRB1*0701-DQB1*0202 and DRB1*1302-DQB1*0609. The results obtained in this study will provide useful information for anthropology, organ transplantation and disease association studies.

show abstract

“…The typing of HLA-A, B and DR B1 was performed through PCR-SSP [1] and reverse line immobilized PCR-SSOP [2] [3]. The significant differences between linkage disequilibria values and 0 are evaluated through the calculation of χ 2 using the Mickey formula [4].…”

Section: Materials and Methods Research Objectmentioning

confidence: 99%

The Genetics Characteristics of HLA Alleles and Haplotypes in the Shanghai Han Population

Feng¹,

Xie²,

et al. 2005

View full text Add to dashboard Cite

Multilocus HLA haplotypes could be investigated by family analysis, and there are salient differences in the distributions of HLA alleles among different populations. In the present study, HLA -A, B and DRB1 alleles and haplotypes were investigated based on 166 families in Shanghai Han population by molecular biological HLA typing methods and the distribution characteristic of HLA alleles and haplotypes were analyzed. The results of our investigation showed that allele frequencies of more than 10% for HLA alleles were A*0201/07, A*1101, A*2402, B*4001, B*4601, DRB1*090102, DRB1*1202 and DRB1*15. In the analysis of HLA haplotypes, we identified 185 kinds of A-B haplotypes , 241 kinds of B-DRB1 haplotypes and 164 kinds of A-DRB1 haplotypes. Fifteen kinds of A-B haplotypes and 15 kinds of B-DRB1 haplotypes and 7 kinds of A-DRB1 haplotypes occurred at frequencies of more than 0.5% (linkage disequilibrium value ∆ > 0, χ 2> 6.63). Three hundred eighty-three kinds of A-B-DRB1 haplotypes were found and 20 kinds of A-B-DRB1 haplotypes occurred at frequencies of more than 0.5% (∆ > 0). The common A-B-DRB1 haplotypes were A*(1.1%). Comparison of the distribution of A-B-DRB haplotype among different populations revealed that Shanghai Han population has its own genetic characteristics, but are closed to East Asian populations and show more abundant polymorphism in the distribution of HLA alleles compare to East Asian populations. The result obtained in this study will be useful to provide information and instruction on Shanghai Han population for genetics, anthropology, association in diseases and forensic paternity testing. Equally encouraging is the potential benefit in helping patients search out healthy, matching hematopoietic stem cells.

show abstract

A three‐step allele‐level DRB1‐DRB3‐DRB4‐DRB5 genotyping assay using polymerase chain reaction with immobilized sequence‐specific oligoprobes

Cited by 13 publications

References 17 publications

HLA-DRB1 and -DRB3 allele frequencies and haplotypic associations in Koreans

HLA-DRB1 and -DRB3 allele frequencies and haplotypic associations in Koreans

HLA class II allele and haplotype frequencies in Koreans based on 107 families

The Genetics Characteristics of HLA Alleles and Haplotypes in the Shanghai Han Population

Contact Info

Product

Resources

About