A tiered barcode authentication tool to differentiate medicinal Cassia species in India

Purushothaman, Nirmala; Sg, Newmaster; Ragupathy, Subramanyam; Stalin, N; Suresh, D.; Dr, Arunraj; Gnanasekaran, Gunadayalan; Sl, Vassou; Narasimhan, D.; Parani, Madasamy

doi:10.4238/2014.april.16.4

Cited by 41 publications

(24 citation statements)

References 31 publications

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“…The objective of their paper was to find the most universal marker for medicinal plant identification, which is different from some of the following examples where barcoding was used to identify selected species from complexes of intended species and their substitutes. Recent research on Cassia [68], Ginkgo [69], Hypericum [70] and many other species [71] has shown great utility in using DNA barcodes for the authentication of ingredients in herbal medicines and related natural health products (NHPs).…”

Section: Sanger Dna Sequencingmentioning

confidence: 99%

DNA Barcoding and Pharmacovigilance of Herbal Medicines

2015

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Pharmacovigilance of herbal medicines relies on the product label information regarding the ingredients and the adherence to good manufacturing practices along the commercialisation chain. Several studies have shown that substitution of plant species occurs in herbal medicines, and this in turn poses a challenge to herbal pharmacovigilance as adverse reactions might be due to adulterated or added ingredients. Authentication of constituents in herbal medicines using analytical chemistry methods can help detect contaminants and toxins, but are often limited or incapable of detecting the source of the contamination. Recent developments in molecular plant identification using DNA sequence data enable accurate identification of plant species from herbal medicines using defined DNA markers. Identification of multiple constituent species from compound herbal medicines using amplicon metabarcoding enables verification of labelled ingredients and detection of substituted, adulterated and added species. DNA barcoding is proving to be a powerful method to assess species composition in herbal medicines and has the potential to be used as a standard method in herbal pharmacovigilance research of adverse reactions to specific products.

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Section: Sanger Dna Sequencingmentioning

confidence: 99%

DNA Barcoding and Pharmacovigilance of Herbal Medicines

2015

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“…Intergenic regions are useful sources for InDels, because these regions underwent relatively rapid mutation due to the less evolutionary constraint. Chloroplast DNA markers for authentication have demonstrated their roles in many aspects as shown in previous studies (Little and Jeanson 2013;Purushothaman et al 2014;Moon et al 2016;Vassou et al 2016). The availability of chloroplast genome sequence to develop the novel loci for DNA markers will become greater in many fields of biology.…”

Section: Discussionmentioning

confidence: 94%

Characterization of Complete Chloroplast Genome of Allium victorialis and Its Application for Barcode Markers

Lee

Chon

Lim

et al. 2017

Plant Breed. Biotech.

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Chloroplast genome sequencing has served as valuable source for developing DNA markers, including the authentication of plant material used for health supplement from its fraudulent materials. We sequenced and analyzed the chloroplast genome of Allium victorialis, a medicinal plant, to discover potential marker regions for the authentication from Veratrum patulum, an inedible toxic plant. Although we examined conventional barcode marker loci in chloroplast, matK and rbcL, there was a difficulty in aligning coding regions and determining PCR primer sequences in these two loci between A. victorialis and V. patulum, possibly due to the distant evolutionary relationship. Instead, we identified potential DNA markers that carry Insertion/Deletion (InDels) that are able to discriminate these two species around clpP, petB, petD, rpl22, and ycf2 loci. In this analysis, we demonstrated the possibility of developing potential DNA markers in the chloroplast genome other than conventional barcode markers, such as matK and rbcL. The potential DNA markers identified in this analysis will serve as useful tools for future authentication of Allium and Veratrum species.

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“…Although individual DNA barcodes were sufficient to discriminate each species, we strongly recommend using both barcode regions, as suggested for discriminating Scutellaria baicalensis from its adulterants in a previous report, because matK and rbcL did not exhibit unique marker nucleotides for A. erubescens and P. pedatisecta, respectively, and multilocus DNA barcodes are more effective for identification of plant species (Stoeckle, 2003;Guo et al, 2011;Purushothaman et al, 2014). In addition to the use of both matK and rbcL barcode regions, we also suggest using phylogenetic analyses to authenticate herbal medicines and their adulterants because phylogenetic clustering provides additional information that can be used for species discrimination on the basis of sequence divergence and co-relationships.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences

Moon

Kim

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Methods to identify Pinelliae Tuber and Arisaematis Rhizoma are required because of frequent reciprocal substitution between these two herbal medicines and the existence of several closely related plant materials. As a result of the morphological similarity of dried tubers, correct discrimination of authentic herbal medicines is difficult by conventional methods. Therefore, we analyzed DNA barcode sequences to identify each herbal medicine and the common adulterants at a species level. To verify the identity of these herbal medicines, we collected five authentic species (Pinellia ternata for Pinelliae Tuber, and Arisaema amurense, A. amurense var. serratum, A. erubescens, and A. heterophyllum for Arisaematis Rhizoma) and six common adulterant plant species. Maturase K (matK) and ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) genes were then amplified using universal primers. In comparative analyses of two DNA barcode sequences, we obtained 45 species-specific nucleotides sufficient to identify each species (except A. erubescens with matK) and 28 marker nucleotides for each species (except P. pedatisecta with rbcL). Sequence differences at corresponding positions of the two combined DNA barcodes provided genetic marker nucleotides that could be used to identify specimens of the correct species among the analyzed medicinal plants. Furthermore, we generated a phylogenetic tree showing nine distinct groups depending on the species. These results can be used to authenticate Pinelliae Tuber and Arisaematis Rhizoma from their adulterants and to identify each species. Thus, comparative analyses of plant DNA barcode sequences identified useful genetic markers for the authentication of Pinelliae Tuber and Arisaematis Rhizoma from several adulterant herbal materials.

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A tiered barcode authentication tool to differentiate medicinal Cassia species in India

Cited by 41 publications

References 31 publications

DNA Barcoding and Pharmacovigilance of Herbal Medicines

DNA Barcoding and Pharmacovigilance of Herbal Medicines

Characterization of Complete Chloroplast Genome of Allium victorialis and Its Application for Barcode Markers

Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences

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