Recently, organic room-temperature phosphorescence (RTP) materials, especially those with reversible responses to external stimuli, have attracted extensive attention. A dynamic regulation strategy enables the materials to rapidly respond to external stimuli, gifting varied RTP performance and greater application potential in sensitive sensing, detection, and so on. For these reasons, this Review summarizes progress in the regulation of dynamic RTP in recent years. It focuses on physical regulatory factors including light, heat, and mechanical force as well as chemical regulatory factors including water, pH, and oxygen. It is expected to be beneficial for developing smart materials with dynamic RTP in the future.