A Time Series of Water Column Distributions and Sinking Particle Flux of Pseudo-Nitzschia and Domoic Acid in the Santa Barbara Basin, California

Umhau, Blaire P.; Benítez-Nelson, Claudia R.; Anderson, Clarissa R.; McCabe, Kimberly A.; Burrell, Christopher

doi:10.3390/toxins10110480

Cited by 18 publications

(13 citation statements)

References 90 publications

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“…The LOD for the two methods was 22 pmol L −1 (Trainer et al, 2009) and 32 pmol L −1 (Guannel et al, 2015) compared to an LOD of 10 pmol DA L −1 in our study. Most field studies quantified dDA using ELISA (Delegrange et al, 2018;Trainer et al, 2009;Trick et al, 2018) whereas LC-MS has only been used sporadically for quantification (Trainer et al, 2000;Umhau et al, 2018). The UPLC-MS/MS method developed here allowed fast quantification and a comprehensive spatial assessment of dDA.…”

Section: Quantification Of Dissolved Domoic Acidmentioning

confidence: 99%

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Quantification, extractability and stability of dissolved domoic acid within marine dissolved organic matter

et al. 2019

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The widespread diatom Pseudo-nitzschia can produce domoic acid (DA). DA is a compound with well described neurotoxic effects on vertebrates including humans known as amnesic shellfish poisoning (ASP) syndrome. It has also been suggested to serve as an organic ligand that binds to iron and copper. By binding these trace elements, DA may increase their solubility and bioavailability. In order to serve this function, DA has to be excreted and reabsorbed by the cells. Only few records of dissolved domoic acid (dDA) concentrations in the ocean exist. To accomplish quantification by ultra performance liquid chromatography (UPLC), samples have to be pre-concentrated and desalted using solid-phase extraction, a procedure commonly applied for dissolved organic matter. Our major goals were to quantify dDA in a basin-wide assessment in the East Atlantic Ocean, to determine extraction efficiencies for complexed and uncomplexed dDA, and to assess whether domoic acid is represented by its molecular formula in direct-infusion high resolution mass spectrometry. Our results showed that dDA was extracted almost quantitatively and occurred ubiquitously in the ocean surface but also in deeper (and older) water, indicating surprisingly high stability in seawater. The maximum concentration measured was 173 pmol L −1 and the average molar dDA carbon yield was 7.7 ppm. Both carbon yield and dDA concentration decreased with increasing water depth. Providing quantification of dDA in the water column, we seek to improve our understanding of toxic bloom dynamics and the mechanistic understanding of DA production.

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Section: Quantification Of Dissolved Domoic Acidmentioning

confidence: 99%

“…Although particulate DA has been frequently measured in marine samples, only few studies covered dDA (Busse et al, 2006;Trainer et al, 2009;Umhau et al, 2018). Previous dDA quantification in seawater was based on a derivatisation procedure and subsequent quantification via HPLC and fluorescence detection (Pocklington et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Quantification, extractability and stability of dissolved domoic acid within marine dissolved organic matter

et al. 2019

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“…Moreover, Pseudonitzschia blooms were found to be associated with high DA levels in the eddy area of Juan de Fuca (Trainer et al, 2002, reviewed by Trainer et al, 2012. Umhau et al (2018) found a significant correlation between particulate DA and Pseudo-nitzschia abundance in an offshore area of Santa Barbara Basin, California (37 ± 124 pg DA cell -1 ). In the Bay of Seine, France, high DA concentrations were correlated with Pseudo-nitzschia australis, P. pungens and P. fraudulenta, but no correlation was found with a bloom of P. delicatissima (Thorel et al, 2017).…”

Section: Discussionmentioning

confidence: 85%

“…through enhanced stratification and nutrient (ammonium) input. Furthermore, possible influences of biotic factors including grazers (Leandro et al, 2010;Tammilehto et al, 2012;Selander et al, 2019), allelopathy (Van Meerssche et al, 2018) and bacteria (Lelong et al, 2012;Sison-Mangus et al, 2014) were not determined and therefore need to be addressed in future studies. pDA and Pseudo-nitzschia spp.…”

Section: Discussionmentioning

confidence: 99%

“…Environmental properties that promote the proliferation of Pseudonitzschia are very complex and vary between regions and seasons https://doi.org/10.1016/j.hal.2019.101708 Received 2 April 2019; Received in revised form 4 November 2019; Accepted 5 November 2019 (Trainer et al, 2012;Umhau et al, 2018). This complexity and ambiguous causative factors may be related to natural climate variability (Sekula-Wood et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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First record of the dynamics of domoic acid producing Pseudo-nitzschia spp. in Indonesian waters as a function of environmental variability

et al. 2019

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Within the past few decades, harmful algal blooms (HABs) have occurred frequently in Indonesian waters, resulting in environmental degradation, economic loss and human health problems. So far, HAB related studies mainly addressed ecological traits and species distribution, yet toxin measurements were virtually absent for Indonesian waters. The aim of the present study was to explore variability of the potentially toxic marine diatom genus Pseudo-nitzschia, as well as its neurotoxin domoic acid as a function of environmental conditions in Ambon Bay, eastern Indonesia. Weekly phytoplankton samples, oceanographic (CTD, nutrients) and meteorological (precipitation, wind) parameters were analyzed at 5 stations in the bay during the dry and wet seasons of 2018. Liquid chromatographytandem mass spectrometry (LC-MS/MS) was used to detect particulate DA (pDA). Vegetative cells of Pseudo-nitzschia spp. and pDA were found in 98.6% and 51.4% of the samples, respectively. pDA levels were low, yet detected throughout the campaign, implying that Ambon Bay might potentially be subject to amnesic shellfish poisoning. The highest levels of both Pseudo-nitzschia spp. cell abundance and pDA were found in the wet season, showing a strong positive correlation between both parameters, compared to the dry season, (r = 0.87 and r = 0.66 (p < 0.01), respectively). Statistical analyses revealed that temperature and mixed layer depth positively correlated with Pseudo-nitzschia spp. and pDA during the dry season, while ammonium showed positive correlations in both seasons. This study represents the first successful investigation of the presence and variability of Pseudo-nitzschia spp. and its neurotoxin DA in Indonesian waters.

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Integrative monitoring strategy for marine and freshwater harmful algal blooms and toxins across the freshwater‐to‐marine continuum

Howard¹,

Smith

Caron

et al. 2022

Integr Envir Assess & Manag

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Many coastal states throughout the USA have observed negative effects in marine and estuarine environments caused by cyanotoxins produced in inland waterbodies that were transported downstream or produced in the estuaries. Estuaries and other downstream receiving waters now face the dual risk of impacts from harmful algal blooms (HABs) that occur in the coastal ocean as well as those originating in inland watersheds. Despite this risk, most HAB monitoring efforts do not account for hydrological connections in their monitoring strategies and designs. Monitoring efforts in California have revealed the persistent detection of cyanotoxins across the freshwater‐to‐marine continuum. These studies underscore the importance of inland waters as conduits for the transfer of cyanotoxins to the marine environment and highlight the importance of approaches that can monitor across hydrologically connected waterbodies. A HAB monitoring strategy is presented for the freshwater‐to‐marine continuum to inform HAB management and mitigation efforts and address the physical and hydrologic challenges encountered when monitoring in these systems. Three main recommendations are presented based on published studies, new datasets, and existing monitoring programs. First, HAB monitoring would benefit from coordinated and cohesive efforts across hydrologically interconnected waterbodies and across organizational and political boundaries and jurisdictions. Second, a combination of sampling modalities would provide the most effective monitoring for HAB toxin dynamics and transport across hydrologically connected waterbodies, from headwater sources to downstream receiving waterbodies. Third, routine monitoring is needed for toxin mixtures at the land–sea interface including algal toxins of marine origins as well as cyanotoxins that are sourced from inland freshwater or produced in estuaries. Case studies from California are presented to illustrate the implementation of these recommendations, but these recommendations can also be applied to inland states or regions where the downstream receiving waterbody is a freshwater lake, reservoir, or river. Integr Environ Assess Manag 2023;19:586–604. © 2022 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

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A Time Series of Water Column Distributions and Sinking Particle Flux of Pseudo-Nitzschia and Domoic Acid in the Santa Barbara Basin, California

Cited by 18 publications

References 90 publications

Quantification, extractability and stability of dissolved domoic acid within marine dissolved organic matter

Quantification, extractability and stability of dissolved domoic acid within marine dissolved organic matter

First record of the dynamics of domoic acid producing Pseudo-nitzschia spp. in Indonesian waters as a function of environmental variability

Integrative monitoring strategy for marine and freshwater harmful algal blooms and toxins across the freshwater‐to‐marine continuum

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