A tissue chip with integrated digital immunosensors: In situ brain endothelial barrier cytokine secretion monitoring

Su, Shiuan‐Haur; Song, Yujing; Stephens, Amanda J.; Situ, Muyu; McCloskey, Molly C.; McGrath, James L.; Andjelkovic, Anuska V.; Singer, Benjamin H.; Kurabayashi, Katsuo

doi:10.1016/j.bios.2022.115030

Cited by 17 publications

(22 citation statements)

References 40 publications

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“…The two types of chip devices have different advantages, discussed in the following paragraphs (Table 1). [2][3][4][5][6]21,[24][25][26][27][28][29][30][31][32][33][34][35][36][37] 2.1 LOC devices with membrane or scaffold-separated channels These LOC devices generally consist of a top and bottom channel, which are separated by the porous cell culture membrane. This geometry enables transendothelial electrical resistance (TEER) measurements with quasi-direct current (DC) or electrical impedance spectroscopic methods, permeability assays and constant monitoring of the cells via phase contrast microscopy.…”

Section: Evolution Of Loc Devices To Study the Bbbmentioning

confidence: 99%

“…Table 1 The major types of chip devices and their parameters to model the blood-brain barrier [2][3][4][5][6]21,[24][25][26][27][28][29][30][31][32][33][34][35][36][37] Prabhakarpandian et al 21 reported the first channel system with porous walls as cell-support. The two 200-μm wide side channels were connected to a large central compartment through 50-μm long and 3-μm wide channels.…”

Section: Evolution Of Loc Devices To Study the Bbbmentioning

confidence: 99%

See 1 more Smart Citation

Lab-on-a-chip models of the blood–brain barrier: evolution, problems, perspectives

Deli,

Porkoláb,

Kincses

et al. 2024

Lab Chip

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Section: Evolution Of Loc Devices To Study the Bbbmentioning

confidence: 99%

Section: Evolution Of Loc Devices To Study the Bbbmentioning

confidence: 99%

Lab-on-a-chip models of the blood–brain barrier: evolution, problems, perspectives

Deli,

Porkoláb,

Kincses

et al. 2024

Lab Chip

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show abstract

“…A good example of the microscopic technique is a BBBoC with integrated digital immunosensors to determine cytokine release, as described in a recent paper [ 58 ]. Named DigiTACK, this device allowed the sequential multiplexed cytokine profiling of primary mouse brain endothelial cells grown as monolayers on a nanoporous silicon nitride membrane.…”

Section: Optical Biosensors As Promising Candidates For Incorporated ...mentioning

confidence: 99%

“…Named DigiTACK, this device allowed the sequential multiplexed cytokine profiling of primary mouse brain endothelial cells grown as monolayers on a nanoporous silicon nitride membrane. The luminal and basal levels of interleukin-6, CCL2/MCP-1 and CXCL1/KC cytokines were detected at three time-points using a digital immunosensor signal readout process by fluorescent microscopy [ 58 ].…”

Section: Optical Biosensors As Promising Candidates For Incorporated ...mentioning

confidence: 99%

“…While at present, integrated sensors in BBBoCs measuring anything other than electrical signals are restricted to detecting oxygen [ 37 ] and cytokines [ 58 ], there is an urgent need to integrate further biosensors into future BBB-model platforms that are capable to detect other substances as well. Molecules of various size and electric charge used for permeability assays, such as labeled dextrans, rhodamine dyes, antibodies or albumin, should be routinely measured by integrated biosensors in the future.…”

Section: Future Perspectivementioning

confidence: 99%

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The Use of Sensors in Blood-Brain Barrier-on-a-Chip Devices: Current Practice and Future Directions

et al. 2023

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The application of lab-on-a-chip technologies in in vitro cell culturing swiftly resulted in improved models of human organs compared to static culture insert-based ones. These chip devices provide controlled cell culture environments to mimic physiological functions and properties. Models of the blood-brain barrier (BBB) especially profited from this advanced technological approach. The BBB represents the tightest endothelial barrier within the vasculature with high electric resistance and low passive permeability, providing a controlled interface between the circulation and the brain. The multi-cell type dynamic BBB-on-chip models are in demand in several fields as alternatives to expensive animal studies or static culture inserts methods. Their combination with integrated biosensors provides real-time and noninvasive monitoring of the integrity of the BBB and of the presence and concentration of agents contributing to the physiological and metabolic functions and pathologies. In this review, we describe built-in sensors to characterize BBB models via quasi-direct current and electrical impedance measurements, as well as the different types of biosensors for the detection of metabolites, drugs, or toxic agents. We also give an outlook on the future of the field, with potential combinations of existing methods and possible improvements of current techniques.

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Leveraging Organ‐on‐Chip Models to Investigate Host–Microbiota Dynamics and Targeted Therapies for Inflammatory Bowel Disease

Kaden,

Alonso‐Román,

Stallhofer

et al. 2024

Adv Healthcare Materials

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Inflammatory bowel disease (IBD) is an idiopathic gastrointestinal disease with drastically increasing incidence rates. Due to its multifactorial etiology, a precise investigation of the pathogenesis is extremely difficult. Although reductionist cell culture models and more complex disease models in animals have clarified the understanding of individual disease mechanisms and contributing factors of IBD in the past, it remains challenging to bridge research and clinical practice. Conventional 2D cell culture models cannot replicate complex host–microbiota interactions and stable long‐term microbial culture. Further, extrapolating data from animal models to patients remains challenging due to genetic and environmental diversity leading to differences in immune responses. Human intestine organ‐on‐chip (OoC) models have emerged as an alternative in vitro model approach to investigate IBD. OoC models not only recapitulate the human intestinal microenvironment more accurately than 2D cultures yet may also be advantageous for the identification of important disease‐driving factors and pharmacological interventions targets due to the possibility of emulating different complexities. The predispositions and biological hallmarks of IBD focusing on host–microbiota interactions at the intestinal mucosal barrier are elucidated here. Additionally, the potential of OoCs to explore microbiota‐related therapies and personalized medicine for IBD treatment is discussed.

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A tissue chip with integrated digital immunosensors: In situ brain endothelial barrier cytokine secretion monitoring

Cited by 17 publications

References 40 publications

Lab-on-a-chip models of the blood–brain barrier: evolution, problems, perspectives

Lab-on-a-chip models of the blood–brain barrier: evolution, problems, perspectives

The Use of Sensors in Blood-Brain Barrier-on-a-Chip Devices: Current Practice and Future Directions

Leveraging Organ‐on‐Chip Models to Investigate Host–Microbiota Dynamics and Targeted Therapies for Inflammatory Bowel Disease

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