A tonic gamma-aminobutyric acid-mediated inhibition of cholinergic amacrine cells in rabbit retina

Massey, Steve; Redburn, Dianna A.

doi:10.1523/jneurosci.02-11-01633.1982

Cited by 95 publications

(90 citation statements)

References 89 publications

(45 reference statements)

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“…The concept of tonic GABA release and modulation of GABAergic activity as a mechanism for regulating transmitter release in the retina has been proposed earlier (Massey and Redbum, 1982;Miller et al, 1981a;Neal and Massey, 1980). In the rabbit retina, bicuculline and picrotoxin enhance basal efflux as well as light-evoked release of 3H-ACh, whereas GABA and muscimol depress release of 3H-ACh (Massey and Redbum, 1982;Neal and Massey, 1980).…”

Section: Discussionmentioning

confidence: 99%

Factors affecting release of 3H-dopamine from perfused carp retina

OʼConnor¹,

Dorison²,

Watling³

et al. 1986

J. Neurosci.

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The effects of putative retinal neurotransmitters and several neuropeptides on 3H-dopamine release from isolated perfused carp (Cypriuus curpio) retinas were studied. Of the transmitter candidates tested, only serotonin (S-HT) and the 5-HT agonist tryptamine released "H-dopamine. However, the release evoked by these agents was calcium (Ca*+) independent and not blockable by the 5-HT antagonist methysergide. We also investigated the antagonism of inhibitory inputs as a potential regulatory mechanism for dopamine release and found that the GABA antagonists, bicuculline and picrotoxin, stimulated a dose-dependent release of 3Hdopamine. The effects of the GABA antagonists were dependent on extracellular Ca*+ and could be inhibited by perfusion of the retina with GABA. Bicuculline and picrotoxin also stimulated an increase in CAMP accumulation, which was inhibited by the dopamine antagonist haloperidol. Our results support the hypothesis that the dopaminergic interplexiform cells of the teleost retina are under GABAergic inhibitory control.

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Section: Discussionmentioning

confidence: 99%

Factors affecting release of 3H-dopamine from perfused carp retina

OʼConnor¹,

Dorison²,

Watling³

et al. 1986

J. Neurosci.

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“…The procedure for continuous superfusion of the rabbit retina, in vivo, has been described in detail previously (Massey & Redburn, 1982). Briefly, a support ring was sutured to the eye, and the cornea, iris, lens, and vitreous humor were removed.…”

Section: Measurement Of Dopamine Releasementioning

confidence: 99%

Dopaminergic modulation of tracer coupling in a ganglion-amacrine cell network

et al. 2007

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Many retinal ganglion cells are coupled via gap junctions with neighboring amacrine cells and ganglion cells. We investigated the extent and dynamics of coupling in one such network, the OFF α ganglion cell of rabbit retina and its associated amacrine cells. We also observed the relative spread of Neurobiotin injected into a ganglion cell in the presence of modulators of gap junctional permeability. We found that gap junctions between amacrine cells were closed via stimulation of a D 1 dopamine receptor, while the gap junctions between ganglion cells were closed via stimulation of a D 2 dopamine receptor. The pairs of hemichannels making up the heterologous gap junctions between the ganglion and amacrine cells were modulated independently, so that elevations of cAMP in the ganglion cell open the ganglion cell hemichannels, while elevations of cAMP in the amacrine cell close its hemichannels. We also measured endogenous dopamine release from an eyecup preparation and found a basal release from the dark-adapted retina of approximately 2 pmol/min during the day. Maximal stimulation with light increased the rate of dopamine release from rabbit retina by 66%. The results suggest that coupling between members of the OFF α ganglion cell/ amacrine cell network is differentially modulated with changing levels of dopamine.

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“…Previous studies indicate that greater than 90% of the radioactivity released in response to glutamate agonists is [ 3 H]-ACh and that virtually all the radioactivity released in response to light is [ 3 H]-ACh (Masland and Livingstone, 1976;Massey and Redburn, 1982). When comparisons are made to previous studies, peak to base ratios are calculated as the peak CPM value during or after the stimuli divided by the average of the CPM values 4 minutes prior to the stimulus.…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

“…The preparation for the continuous superfusion of the rabbit retina in vivo has been described in detail previously (Massey and Redburn, 1982). Briefly, a support ring was attached to the eye so that the cornea, lens, and vitreous could be removed, leaving the intact retina lining the eyecup.…”

Section: Physiologymentioning

confidence: 99%

AMPA receptors mediate acetylcholine release from starburst amacrine cells in the rabbit retina

Firth

Massey

et al. 2003

J of Comparative Neurology

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The light response of starburst amacrine cells is initiated by glutamate released from bipolar cells. To identify the receptors that mediate this response, we used a combination of anatomical and physiological techniques. An in vivo, rabbit eyecup was preloaded with [ 3 H]-choline, and the [ 3 H]-acetylcholine (ACh) released into the superfusate was monitored. A photopic, 3 Hz flashing light increased ACh release, and the selective AMPA receptor antagonist, GYKI 53655, blocked this light-evoked response. Nonselective AMPA/kainate agonists increased the release of ACh, but the specific kainate receptor agonist, SYM 2081, did not increase ACh release. Selective AMPA receptor antagonists, GYKI 53655 or GYKI 52466, also blocked the responses to agonists. We conclude that the predominant excitatory input to starburst amacrine cells is mediated by AMPA receptors. We also labeled lightly fixed rabbit retinas with antisera to choline acetyltransferase (ChAT), AMPA receptor subunits GluR1, GluR2/3, or GluR4, and kainate receptor subunits GluR6/7 and KA2. Labeled puncta were observed in the inner plexiform layer with each of these antisera to glutamate receptors, but only GluR2/3-IR puncta and GluR4-IR puncta were found on the ChAT-IR processes. The same was true of starburst cells injected intracellularly with Neurobiotin, and these AMPA receptor subunits were localized to two populations of puncta. The AMPA receptors are expected to desensitize rapidly, enhancing the sensitivity of starburst amacrine cells to moving or other rapidly changing stimuli. Indexing termsglutamate; ionotropic receptors; localization; cholinergic; interneuron; confocal microscopy Starburst amacrine cells are one of the most common types of amacrine cells in rabbit retina (MacNeil et al., 1999), and there are two subtypes. Type a cells have perikarya in the inner nuclear layer, processes ramifying in the outer half of the inner plexiform layer (IPL) in stratum 2 (S2) and OFF responses to light. Type b cells have perikarya displaced to the ganglion cell layer, processes ramifying in stratum 4 (S4) of the IPL and ON responses to light (Famiglietti, 1983;Tauchi and Masland, 1984;Vaney, 1984;Bloomfield and Miller, 1986). In rabbits, starburst amacrine cells are known to receive synaptic inputs from cone (Brandon, 1987;Millar and Morgan, 1987;Famiglietti, 1991).Starburst amacrine cells synthesize and release both γ-aminobutyric acid (GABA) and acetylcholine (ACh) when depolarized (Masland and Livingstone, 1976;Massey and Neal, 1979;O'Malley and Masland, 1989). ACh release from the rabbit retina increases at both light onset and light offset and is maximally stimulated by large stimuli at temporal frequencies of 1-4 Hz (Massey and Neal, 1979;Massey and Redburn, 1985;O'Malley and Masland, 1993). The majority of the synaptic output from the starburst cells is onto ganglion cell dendrites (Brandon, 1987;Millar and Morgan, 1987;Famiglietti, 1991 . The agonist α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) is more potent than kainate or...

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A tonic gamma-aminobutyric acid-mediated inhibition of cholinergic amacrine cells in rabbit retina

Cited by 95 publications

References 89 publications

Factors affecting release of 3H-dopamine from perfused carp retina

Factors affecting release of 3H-dopamine from perfused carp retina

Dopaminergic modulation of tracer coupling in a ganglion-amacrine cell network

AMPA receptors mediate acetylcholine release from starburst amacrine cells in the rabbit retina

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