2019
DOI: 10.1101/808329
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A toolbox of Stable Integration Vectors (SIV) in the fission yeastSchizosaccharomyces pombe

Abstract: Schizosaccharomyces pombe is a widely used model organism that resembles higher eukaryotes in many aspects of cell physiology. Its popularity as an experimental system partially stems from the ease of genetic manipulations, where the innate homology-targeted repair is exploited to precisely edit the genome. While vectors to incorporate exogenous sequences into the chromosomes are available, most are poorly characterized. Here we show that commonly used fission yeast vectors, which upon integration produce repe… Show more

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Cited by 6 publications
(1 citation statement)
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“…Gene tagging was performed at endogenous genomic locus at the 3′ end, yielding C-terminally tagged proteins, as described [ 35 ]. Pak1 gene tagging was performed by transforming a WT strain with AfeI linearized (pBSII(KS + ))-based single integration vector (pAV72-3′UTR pak1 -Pak1-sfGFP-kanMX-5′UTR pak1 ) targeting the endogenous locus [ 36 ]. The functional mCherry-tagged and sfGFP-tagged cdc42 alleles cdc42-mCherry sw and cdc42-sfGFP sw were used as described in [ 4 ].…”
Section: Methodsmentioning
confidence: 99%
“…Gene tagging was performed at endogenous genomic locus at the 3′ end, yielding C-terminally tagged proteins, as described [ 35 ]. Pak1 gene tagging was performed by transforming a WT strain with AfeI linearized (pBSII(KS + ))-based single integration vector (pAV72-3′UTR pak1 -Pak1-sfGFP-kanMX-5′UTR pak1 ) targeting the endogenous locus [ 36 ]. The functional mCherry-tagged and sfGFP-tagged cdc42 alleles cdc42-mCherry sw and cdc42-sfGFP sw were used as described in [ 4 ].…”
Section: Methodsmentioning
confidence: 99%