A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism

Abstract

BackgroundExpression of the CNDP2 gene is frequently up- or down-regulated in different types of human cancers. However, how the product of this gene is involved in cell growth and proliferation is poorly understood. Moreover, our knowledge of the functions of the CNDP2 orthologs in well-established model organisms is scarce. In particular, the function of the D. melanogaster ortholog of CNDP2, encoded by the CG17337 gene (hereafter referred to as dCNDP2), is still unknown.ResultsThis study was aimed at developing a set of genetic and molecular tools to study the roles of dCNDP2. We generated a dCNDP2 null mutation (hereafter ∆dCNDP2) using CRISPR/Cas9-mediated homologous recombination (HR) and found that the ∆dCNDP2 mutants are homozygous viable, morphologically normal and fertile. We also generated transgenic fly lines expressing eGFP-tagged and non-tagged dCNDP2 protein, all under the control of the UAS promoter, as well as polyclonal antibodies specific to dCNDP2. Using these tools, we demonstrate that only one of the two predicted dCNDP2 isoforms is expressed throughout the different tissues tested. dCNDP2 was detected in both the cytoplasm and the nucleus, and was found to be associated with multiple sites in the salivary gland polytene chromosomes.ConclusionsThe dCNDP2 gene is not essential for fly viability under standard laboratory conditions. The subcellular localization pattern of dCNDP2 suggests that this protein might have roles in both the cytoplasm and the nucleus. The genetic and molecular tools developed in this study will allow further functional characterization of the conserved CNDP2 protein using D. melanogaster as a model system.Electronic supplementary materialThe online version of this article (10.1186/s12863-019-0726-z) contains supplementary material, which is available to authorized users.

Proteomic analysis of extracellular vesicles identified PI3K pathway as a potential therapeutic target for cabazitaxel‐resistant prostate cancer

“…Interestingly, increased expression of CD151 on EVs was reported as a driver of metastasis in PC 28 . CNDP2 gene expression is up‐ or downregulated in several types of cancer, however, its pathological role is still unclear 29‐31 . For MYL9, it has been reported that PC progression was promoted by its decreased expression in prostate stromal tissues, 32 although inconsistent with our finding.…”

“…28 CNDP2 gene expression is up-or downregulated in several types of cancer, however, its pathological role is still unclear. [29][30][31] For MYL9, it has been reported that PC progression was promoted by its decreased expression in prostate stromal tissues, 32 although inconsistent with our finding. Increased expression of PRDX6 was shown to correlate with progression and chemotherapy-resistance in several cancers such as cervical cancer and lung cancer.…”

Zinc oxide nanoparticles induce oxidative stress, genotoxicity, and apoptosis in the hemocytes of Bombyx mori larvae

“…Up-regulation of the CNDP 2 gene was detected in colon cancer cell lines 31 . However, research on Drosophila melanogaster revealed that the CNDP-2 gene is located in the nucleus, where it can be associated with chromatin, implying that it plays a role in DNA replication, transcription, or repair 32 . In this work, CNDP2 was significantly up-regulated after the exposure of larvae to ZnO-NPs, suggesting an alteration in the cell cycle growth of the worm.…”

Disruption of a C69-Family Cysteine Dipeptidase Gene Enhances Heat Shock and UV-B Tolerances in Metarhizium acridum

“…At present, the research of dipeptidase mainly focuses on animal (O'Dwyer et al, 2009;Andreyeva et al, 2019;Choudhury et al, 2019), human pathogenic bacteria (Chang et al, 2010;Toyotome et al, 2015) or plant pathogenic bacteria (Kumar et al, 2014). Function reports of dipeptidases are mostly limited to virulence and growth in microorganisms, while there is little information regarding the effect of dipeptidases on stress tolerance in microorganisms.…”