A Total Content of Phenolic Compounds in the Ethanol Extracts of Galega Officinalis L. and G. Orientalis Lam.

Shymanska, Oksana

doi:10.15414/agrobiodiversity.2018.2585-8246.140-145

Cited by 8 publications

(9 citation statements)

References 5 publications

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“…For the assessment of the total phenolic and flavonoid content, as well as for the antioxidant activity evaluation, 0.2 g of the berries were freeze-dried, milled, and extracted with 20 mL 80% ethanol (Centralchem, Bratislava, Slovakia) for 24 h. After centrifugation (4000 rpm, 20 min), the supernatant was collected and used for further experiments [ 15 ]. In case of the quantification of total carotenoids, 0.5 g of dry Omija fruit was homogenized in the mortar with sea sand and repeatedly extracted with 10 mL acetone (Centralchem) until the sample became colourless.…”

Section: Methodsmentioning

confidence: 99%

“…The total phenolic content of the Omija extract was measured using the Folin–Ciocalteu method [ 15 , 16 ]. One hundred mL of the extract was mixed with 0.1 mL of the Folin–Ciocalteu reagent (Sigma-Aldrich), 1 mL 20% (w/v) sodium carbonate (Centralchem), and 8.8 mL distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…After 30 min in darkness, the absorbance was measured in triplicates at 700 nm using the Jenway 6405 UV/Vis spectrophotometer (Cole-Pharmer Ltd.). Gallic acid (25–300 mg/L; Sigma-Aldrich) was used as the standard, and the results were expressed in mg gallic acid equivalents (GAE)/g d.w. [ 15 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

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Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage

Tvrdá

Michalko

Árvay

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Schisandra chinensis is a woody vine native to China, Korea, and Russia, which has been used as a traditional herbal remedy to treat male infertility. As very little information is available concerning its effects on ejaculated spermatozoa, the aim of this study was to investigate the chemical, antioxidant, and antibacterial properties of the S. chinensis berry (Omija) extract followed by an assessment of its in vitro effects on bovine sperm function and oxidative balance. Phytochemical components of the Omija extract were determined by high performance liquid chromatography. The content of polyphenols, flavonoids, and carotenoids was assessed by spectrophotometric protocols. Antioxidant characteristics of the Omija extract were determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and molybdenum-reducing antioxidant power (MRAP) assays. The disc diffusion method and determination of the minimal inhibitory concentration were applied to study the antibacterial properties of Schisandra. Thirty semen samples were exposed to different concentrations of Omija (1, 5, 10, 25, 50, and 75 µg/mL) for 0, 2, and 24 h. Sperm motility, mitochondrial activity, and superoxide and reactive oxygen species production, as well as total antioxidant capacity and oxidative damage to proteins and lipids were determined. Our data reveal that the Omija extract, particularly at a concentration range within 5–50 µg/mL, exhibited dose-dependent motion-promoting and metabolism-enhancing properties, accompanied by significant antioxidant effects. We may conclude that the biomolecules present in the Omija extract such as schisandrins and phenolic molecules offer protection to critical sperm structures against oxidative insults and/or possible bacterial contamination, leading to a higher preservation of mammalian sperm viability and functional activity.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage

Tvrdá

Michalko

Árvay

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Medicinal herbs are used due to their health benefits, special aroma, taste and are considered one of the richest sources of bioactive compounds (Shanayda and Korablova, 2015;Shymanska et al, 2018;Mňahončáková et al, 2019;Ivanišová et al, 2017Ivanišová et al, , 2020. The commercial development of plants as sources of antioxidants to enhance health and food Effect of alginite in the form of ALGEX r 6 preparation on the biomass formation and antioxidant activity of some medicinal plants preservation is of current interest (Rice-Evans et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Effect of Alginite in the Form of ALGEXr 6 Preparation on the Biomass Formation and Antioxidant Activity of Some Medicinal Plants

Sedláčková

Šimková²,

Mňahončáková³

et al. 2021

Agrobiodivers Improv Nutr health life Qual

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The aim of the research is to determine the effect of application of the developed product (extract) called ALGEX r 6 from natural mineral rock alginite in two different watering periods on the formation of root biomass and aboveground plant biomass of a selected group of medicinal plants (Melissa officinalis L., Malva verticillata L. and Ocimum × citriodorum Vis.) and determining the antioxidant activity in dried leaves and whole plants in aqueous and methyl alcohol extracts by DPPH method. The experiment was established in the Botanical Garden at the Slovak University of Agriculture in Nitra throughout 2020. ALGEX r 6 was prepared by a research team at the Slovak University of Agriculture in Nitra in the form of an extract from the natural mineral rock alginite with an application of thermal and chemical treatment. In the experiment, ALGEX r 6 was applied in the form of a watering in two variants with the same concentration of 3 % solution in 2 decilitres of water, but various application in terms of days in the pre-harvest stage of the above-ground plant biomass of 30 individual plants from each species. There are two diametrically opposite trends of ALGEX r 6 application that are manifesting themselves in M. officinalis and M. verticillata by reducing the root and above-ground part biomass compared to the control variant. The percentage proportionality of root/ above-ground part biomass in M. officinalis decreased from 62.48/30.31 % (control), to 45.57/18.85 % (variant 1) and to 36.07/17.27 % (variant 2), as well as in M. verticillata the root/above-ground part biomass decreased from 16.03/13.93 % (control), to 14.97/9.42 % (variant 1) and to 11.61/10.14 % (variant 2). In the species Ocimum × citriodorum Vis. the opposite trend manifested. The application of ALGEX r 6 watering resulted in increasing the antioxidant activity on the tested plant parts, especially in aqueous extracts in M. officinalis (from 19.30/control to 33.61 %/variant 1, 2), also in O. citriodorum (from 26.56/control to 44.16 %/variant 1), while in methyl alcohol extracts, the antioxidant activity showed a slight increase in all tested species.

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“…Legumes are a good source of nutrients such as proteins, carbohydrates, dietary fibers, micronutrients, vitamins, fatty acids (Guttiérez-Uribe et al, 2016). Some representatives from Fabaceae well-known forage plants with a high content of polyphenol compounds and antioxidant activity (Shymanska et al, 2018;. The screening of some legumes plants determined the antimicrobial activity against gram-positive bacteria, Candida albicans, Aspergillus niger and Pseudomonas aeruginosa (Rosado-Vallado et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

DPPH Free Radical Scavenging Activity of Some Fabaceae Lindl. Species

Vergun¹

2020

Agrobiodiversity for Improving Nutrition, Health and Life Quality

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Skif-1 at the flowering stage. Raw material was collected from the experimental collections of M.M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG) in the stage of flowering. Determination of the antioxidant activity of investigated plants conducted by DPPH free radical scavenging activity according to Brand-Williams et al. (1995). The total antioxidant activity of plant whole above-ground part of methanol extracts was from 62.78 (Coronilla varia) to 89.99 (Glycyrrhiza glabra) % and water extracts from 51.28 (Onobrychis grandis) to 90.35 (Lespedeza bicolor) %. Leaf extracts exhibited DPPH scavenging activity in methanol extracts from 63.17 (Trifolium ambiguum) to 93.08 (Onobrychis arenaria) % and water extracts from 57.62 (Coronilla varia) to 95.36 (Lathyrus grandiflorus) %. Stem extracts of investigated plants showed inhibition in methanol extracts from 35.04 (Coronilla varia) to 90.51 (Glycyrrhiza glabra) % and water extracts from 20.35 (Coronilla varia) to 77.52 (Lathyrus grandiflorus) %. DPPH scavenging activity of these plants depended on species and part of plants. Thus, plants from Fabaceae family are a potential source of antioxidant activity that can be used for deep pharmacological investigations for food science.

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A Total Content of Phenolic Compounds in the Ethanol Extracts of Galega Officinalis L. and G. Orientalis Lam.

Abstract: AGROBIODIVERSITY f o r i m p r o v i n g n u t r i t i o n , h e a l t h a n d l i f e q u a l i t y 2018

Cited by 8 publications

References 5 publications

Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage

Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage

Effect of Alginite in the Form of ALGEXr 6 Preparation on the Biomass Formation and Antioxidant Activity of Some Medicinal Plants

DPPH Free Radical Scavenging Activity of Some Fabaceae Lindl. Species

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