This research studies the traces of an ancestral textile produced in karkhanehs (workspaces) located in the historical city of Yazd, Iran. The case study undertaken here demonstrates how an intangible heritage of textile weaving through generations of families in Yazd, Iran, interrelates with tangible vernacular architecture and tourism at three different scales: (i) in everyday life in karkhanehs at home, (ii) in the neighborhoods, and (iii) the UNESCO World Heritage city of Yazd. The three scales related to the enactment of this vernacular handicraft are examined using architectural methods to examine structures ranging from 90 to 600 years in age. This was complemented by discussions with local weavers and residents, as well as direct observation in domiciles, neighborhoods, and the city. Actor-Network Theory helped to trace the networks of actors and relationships between the tangible built architectural heritage and intangible cultural practices of weaving, showing how different genders, ages, worldviews (beliefs) and practices came together to produce this heritage textile. Actor-Network Theory also helped to study the relationships between economy, culture, society and tourism, with respect to the evolution and transformations of the historic urban dwellings, vernacular architecture and vernacular weaving handicrafts through the three scales examined. Implications for sustaining and conserving this ancestral tradition of textile weaving and managing tourism’s positive as well as disruptive influences on cultural heritage conservation are discussed.