A Toxicity Index of Skin and Wound Cleansers Used on In Vitro Fibroblasts and Keratinocytes

Wilson, Judy R.; Mills, John W.; Prather, Irvine D.; Dimitrijevich, Slobodan D.

doi:10.1097/00129334-200509000-00011

Cited by 132 publications

(98 citation statements)

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“…These findings are in opposition to an in vitro study that showed various degrees of cytotoxicity in fibroblasts with the use of soaps (19) . The absence of randomized clinical trials on the use of antiseptics in the topical therapy of chronic wounds reflects the lack of definition with regard to the tendency to use them, so that this is a controversial practice (20)(21)(22).…”

Section: Discussioncontrasting

confidence: 99%

Manejo clínico de úlceras venosas na atenção primária à saúde

et al. 2012

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Objective: To discuss the clinical management of venous ulcers in primary health care, based on the vision of users who live with this affliction. Methods: This was a qualitative, exploratory, descriptive study, conducted with 25 adult users in treatment in the Family Health Units. Data were collected in the second semester of 2008, using a structured instrument with questions of sociodemographic characteristics, type of cleaning, coverings, use of compression therapy, medication and prescribed guidelines. Results: Cleaning products are used that aggravate the granulation tissue, such as coverings with various substances, including sunflower oil and antibiotic ointments; the majority of users do not use measures to control edema. Conclusion: It is necessary to elaborate and adopt clinical protocols for the care of venous ulcers, as well as to provide ongoing education for health professionals. Keywords: Varicose ulcer; Wound healing; Primary health care; Bandages RESUMOObjetivo: Discutir o manejo clínico de úlceras venosas realizado na atenção primária à saúde, com base na visão dos usuários que convivem com esta afecção. Métodos: Estudo de natureza qualitativa exploratório, descritivo, realizado com 25 usuários adultos em tratamento nas Unidades de Saúde da Família. Os dados foram coletados no segundo semestre de 2008, utilizando-se um formulário estruturado com questões de caracterização sociodemográfica, tipo de limpeza, coberturas, uso de terapia compressiva, medicamentos e orientações prescritas. Resultados: São usados para a limpeza produtos que agridem o tecido de granulação, como coberturas com várias substâncias, dentre elas o óleo de girassol e pomadas antibióticas; a maioria dos usuários não utiliza medidas para controle do edema. Conclusão: fazem-se necessárias a elaboração e a adoção de protocolos clínicos para o cuidado com úlceras venosas, bem como a capacitação permanente dos profissionais de saúde. Descritores: Úlcera varicosa; Cicatrização; Atenção primária à saúde; Bandagens RESUMENObjetivo: Discutir el manejo clínico de úlceras venosas realizado en la atención primaria a la salud, con base en la visión de los usuarios que conviven con esta afección. Métodos: Estudio de naturaleza cualitativa exploratorio, descriptivo, realizado con 25 usuarios adultos en tratamiento en las Unidades de Salud de la Familia. Los datos fueron recolectados en el segundo semestre del 2008, utilizándose un formulario estructurado con preguntas de caracterización sociodemográfica, tipo de limpieza, coberturas, uso de terapia compresiva, medicamentos y orientaciones prescriptas. Resultados: Son usados para la limpieza productos que agreden el tejido de granulación, como coberturas con varias sustancias, entre ellas el aceite de girasol y pomadas antibióticas; la mayoría de los usuarios no utiliza medidas para control del edema. Conclusión: Se hace necesaria la elaboración y la adopción de protocolos clínicos para el cuidado con úlceras venosas, así como la capacitación permanente de los profesionales de salud....

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Section: Discussioncontrasting

confidence: 99%

Manejo clínico de úlceras venosas na atenção primária à saúde

et al. 2012

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“…In the report by Wilson et al 2005 that examined a toxicity index of hydrogen peroxide, sodium hypochlorite and povidone iodine, they reported that some of the antiseptics were more toxic in phosphate buffered saline PBS than in medium containing FBS. In another report, Nagamune et al 2000 evaluated the antibacterial activity and acute cytotoxicity of bis-QACs on several types of human cells including fibroblasts, keratinocytes, erythrocyte and lymphoma.…”

Section: Discussionmentioning

confidence: 99%

<i>In Vitro</i> Evaluation of the Biocompatibility of Newly Synthesized Bis-Quaternary Ammonium Compounds with Spacer Structures Derived from Pentaerythritol or Hydroquinone

et al. 2016

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With the object of developing new biocides milder for human use than the current antiseptics, we synthesized a series of bis-quaternary ammonium compounds bis-QACs . The antimicrobial activity of the newly synthesized bis-QACs and common biocides used as antiseptics was compared by examining minimum inhibitory concentrations and minimum bactericidal concentrations MBCs . Moreover, the cytotoxicity of these compounds against human cells was determined to calculate the biocompatibility index BI of these compounds. BI was the ratio of the concentration of a biocide giving a 50% lethal effect on normal human epidermal keratinocytes to its MBC against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The commonly used antiseptics tested were benzalkonium chloride BAC , octenidine dihydrochloride OCT , chlorhexidine digluconate CHG and polyhexamethylene biguanide PHMB . In comparison with these antiseptics, it was shown that some of new bis-QACs exhibited a wider and more potent antimicrobial spectrum than OCT. The cytotoxicity of these bisQACs was equal or lower compared to that of the quaternary ammonium compounds BAC and OCT , although these bis-QACs showed higher toxicity than the biguanide-based compounds CHG and PHMB . Finally, the comparison of BIs revealed that new bis-QACs such as N-dodecyl 4,4 -2,4,8,10-tetraoxaspiro 5.5 undecan-3,9-diyl dipyridinium dibromide 4TOSU-12 , 3,3'-1,4-Phenylenebis oxy bis 1-dodecylpyridinium dibromide 3PHBO-12 and 3-3-Hydroxy-2-hydroxymethyl -2-1-dodecylpyridinium-3-yl oxy methyl propoxy -1-dodecylpyridinium dibromide 3HHDMP-12 had equal or greater biocompatibility than the commonly used biocides tested. Thus, these results strongly suggested that 4TOSU-12, 3PHBO-12 and 3HHDMP-12 could be useful as antiseptics for topical application to the skin.

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“…Chemical products, especially for living tissue, should be evaluated on not only their effectivity but also their toxicity from a practical point of view. For antiseptics which are anti-infective substances especially used for preparations applied to living tissue, several indexes for the evalu- Natural Synergism of Sophorolipids ation of their preparations have been suggested 29,30) . Muller et al have proposed "biocompatibility index" to evaluate the ideal antiseptics which have an effective microbicidal activity combined with a relatively low cytotoxicity 30) .…”

Section: Discussionmentioning

confidence: 99%