A TPR scaffold couples signal detection to OdhI phosphorylation in metabolic control by the protein kinase PknG

Abstract

Signal transduction is essential for bacteria to adapt to changing environmental conditions. Among many forms of post-translational modifications, reversible protein phosphorylation has evolved as a ubiquitous molecular mechanism of protein regulation in response to specific stimuli. The Ser/Thr protein kinase PknG modulates the fate of intracellular glutamate by controlling the phosphorylation status of the 2-oxoglutarate dehydrogenase regulator OdhI, a function that is conserved among diverse actinobacteria. PknG has a modular organization characterized by the presence of regulatory domains surrounding the catalytic domain. Here we present an investigation through in vivo experiments as well as biochemical and structural methods of the molecular bases of the regulation of PknG from C. glutamicum (CgPknG), in the light of previous knowledge available for the kinase from M. tuberculosis (MtbPknG). We found that OdhI phosphorylation by CgPknG is regulated by a conserved mechanism that depends on a C-terminal domain composed of tetratricopeptide repeats (TPR) essential for metabolic homeostasis. Furthermore, we identified a conserved structural motif that physically connects the TPR domain and a flexible N-terminal extension of the kinase that is involved in docking interactions with OdhI. Based on our results and previous reports, we propose a model in which the TPR domain of PknG couples signal detection to the specific phosphorylation of OdhI. Overall, the available data indicate that conserved PknG domains in distant actinobacteria retain their roles in kinase regulation in response to nutrient availability.IMPORTANCEBacteria control the metabolic processes by which they obtain nutrients and energy in order to adapt to the environment. In this way, the metabolic characteristics of a microorganism determine its ecological role and its usefulness in industrial processes. Here, we use genetic, biochemical, and structural approaches to study a key component in a system that regulates glutamate production in C. glutamicum, a species that is used for the industrial production of amino acids. We elucidated molecular mechanisms involved in metabolic control in C. glutamicum, which are conserved in related pathogenic bacteria. The findings have broader significance for diverse actinobacteria, including microorganisms that cause disease as well as environmental species used to produce billions of dollars of amino acids and antibiotics every year.

Cosolvent Sites-Based Discovery ofMycobacterium TuberculosisProtein Kinase G Inhibitors

“…The PknG domain architecture is unique: besides the kinase domain, it has a rubredoxin domain (which may be involved in redox sensing), a tetratricopeptide domain (involved in protein–protein interactions), and a non-structured region of ∼70 residues with four auto-phosphorylatable sites. The three-dimensional structure of the PknG kinase domain has been elucidated, thus turning it into an ideal target for drug screening projects. , In fact, several successful attempts have been made to discover PknG ligands; however, none of the identified compounds have advanced into clinical trials.…”

“…6−8 PknG is one of the 11 serine− threonine protein kinases identified in Mtb, 9 and it regulates the tricarboxylic acid cycle by phosphorylation of protein GarA, regulates response to hypoxia, and is essential for pathogenicity. 10−14 The PknG domain architecture is unique: besides the kinase domain, it has a rubredoxin domain (which may be involved in redox sensing), 15 a tetratricopeptide domain (involved in protein−protein interactions), 16 and a non-structured region of ∼70 residues with four auto-phosphorylatable sites. The three-dimensional structure of the PknG kinase domain has been elucidated, thus turning it into an ideal target for drug screening projects.…”