A tracheal scaffold of gelatin-chondroitin sulfate-hyaluronan-polyvinyl alcohol with orientated porous structure

Yu, Xueping; Qian, Guowen; Chen, Si; Xu, Dong; Zhao, Xiujuan; Du, Chang

doi:10.1016/j.carbpol.2016.12.017

Cited by 31 publications

(34 citation statements)

References 52 publications

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“…For the swelling ratio and water absorption, rectangular block hydrogels of different internal structures, in size of 10 mm × 10 mm × 3.2 mm, were measured according to the reported methods by the authors of [15]. The 3D printed hydrogels were prefrozen at −20 °C in a refrigerator and then freeze-dried in a freeze-drying machine (VIRTIS Genesis, Warminster, PA, USA) to obtain freeze-dried hydrogels.…”

Section: Methodsmentioning

confidence: 99%

Preparation and Properties of 3D Printed Alginate–Chitosan Polyion Complex Hydrogels for Tissue Engineering

Liu

et al. 2018

Polymers

153

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Three-dimensional (3D) printing holds great potential for preparing sophisticated scaffolds for tissue engineering. As a result of the shear thinning properties of an alginate solution, it is often used as 3D printing ink. However, it is difficult to prepare scaffolds with complexity structure and high fidelity, because the alginate solution has a low viscosity and alginate hydrogels prepared with Ca 2+ crosslinking are mechanically weak. In this work, chitosan powders were dispersed and swelled in an alginate solution, which could effectively improve the viscosity of an alginate solution by 1.5–4 times. With the increase of chitosan content, the shape fidelity of the 3D printed alginate–chitosan polyion complex (AlCh PIC) hydrogels were improved. Scanning electron microscope (SEM) photographs showed that the lateral pore structure of 3D printed hydrogels was becoming more obvious. As a result of the increased reaction ion pairs in comparison to the alginate hydrogels that were prepared with Ca 2+ crosslinking, AlCh PIC hydrogels were mechanically strong, and the compression stress of hydrogels at a 90% strain could achieve 1.4 MPa without breaking. In addition, human adipose derived stem cells (hASCs) adhered to the 3D printed AlCh PIC hydrogels and proliferated with time, which indicated that the obtained hydrogels were biocompatible and could potentially be used as scaffolds for tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Preparation and Properties of 3D Printed Alginate–Chitosan Polyion Complex Hydrogels for Tissue Engineering

Liu

et al. 2018

Polymers

153

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“…Figure 6 A shows the viscoelastic properties of the biocomposites when they were subjected to different frequencies in the range 0.1–10 Hz. Specific frequencies were chosen to simulate in vivo stress conditions, evaluating the biocomposites’ behavior in term of storage (E’) and loss (E’’) modulus [ 48 , 49 ]. E’’ are not reported because the values observed were very low in comparison to E’, indicating a predominantly elastic behavior [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Blending Gelatin and Cellulose Nanofibrils: Biocomposites with Tunable Degradability and Mechanical Behavior

et al. 2020

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Many studies show how biomaterial properties like stiffness, mechanical stimulation and surface topography can influence cellular functions and direct stem cell differentiation. In this work, two different natural materials, gelatin (Gel) and cellulose nanofibrils (CNFs), were combined to design suitable 3D porous biocomposites for soft-tissue engineering. Gel was selected for its well-assessed high biomimicry that it shares with collagen, from which it derives, while the CNFs were chosen as structural reinforcement because of their exceptional mechanical properties and biocompatibility. Three different compositions of Gel and CNFs, i.e., with weight ratios of 75:25, 50:50 and 25:75, were studied. The biocomposites were morphologically characterized and their total- and macro- porosity assessed, proving their suitability for cell colonization. In general, the pores were larger and more isotropic in the biocomposites compared to the pure materials. The influence of freeze-casting and dehydrothermal treatment (DHT) on mechanical properties, the absorption ability and the shape retention were evaluated. Higher content of CNFs gave higher swelling, and this was attributed to the pore structure. Cross-linking between CNFs and Gel using DHT was confirmed. The Young’s modulus increased significantly by adding the CNFs to Gel with a linear relationship with respect to the CNF amounts. Finally, the biocomposites were characterized in vitro by testing cell colonization and growth through a quantitative cell viability analysis performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Additionally, the cell viability analysis was performed by the means of a Live/Dead test with Human mesenchymal stem cells (hMSCs). All the biocomposites had higher cytocompatibility compared to the pure materials, Gel and CNFs.

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“…To overcome this problem, we used another polymer (PVA) to stabilize GT . Therefore, by combining GT and PVA in this study, we enjoy both GT and PVA characteristics, and results showed that the GT/PVA scaffold had improved cell behavior. GAGs are linear polysaccharides that have many functions in different cellular mechanisms such as the repair and regeneration process and can be found in cartilage ECM .…”

Section: Discussionmentioning

confidence: 99%

Chondro‐inductive nanofibrous scaffold based gelatin/polyvinyl alcohol/chondroitin sulfate for cartilage tissue engineering

Irani

Honarpardaz

Choubini

et al. 2020

Polymers for Advanced Techs

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Tissue engineering is a technique that can be used for repairing damaged cartilage.The aim of this study was chondro-differentiation of mesenchymal stem cells (MSCs) on polyvinyl alcohol/gelatin/chondroitin sulfate (PVA/GT/CS) blend nanofibers. Nanofibers were fabricated by electrospinning method with different concentrations of CS (10%, 15%, and 20%). MSCs were seeded on nanofibers for biocompatibility and cell viability test by MTT assay. Alcian blue staining assay was done to show that MSCs were differentiated to chondrocyte by staining the glycosaminoglycans (GAGs) that was produced by cells. Reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry (ICC) assay were done to confirm type II collagen expression. Scaffolds with different concentrations of CS were tested for biocompatibility, and the nanofiber with 15% of GAG showed better results (P = 0.02) compared with other nanofibers; therefore, it was chosen as the main sample for chondrogenesis tests. On the basis of scanning electron microscopy (SEM), hMSCs had a proper attachment to the nanofiber with 15% CS. Alcian blue staining showed the different rates of blue coloration, which means cells produced different types of GAGs from the GAG we used in the nanofiber. RT-PCR and ICC showed that COL2A1 was expressed in mRNA and protein levels. This study indicates that the PVA/GT/CS nanofiber containing 15% CS can be a good candidate for cartilage tissue engineering. K E Y W O R D S cartilage, chondroitin sulfate, electrospun, mesenchymal stem cells, tissue engineering

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A tracheal scaffold of gelatin-chondroitin sulfate-hyaluronan-polyvinyl alcohol with orientated porous structure

Cited by 31 publications

References 52 publications

Preparation and Properties of 3D Printed Alginate–Chitosan Polyion Complex Hydrogels for Tissue Engineering

Preparation and Properties of 3D Printed Alginate–Chitosan Polyion Complex Hydrogels for Tissue Engineering

Blending Gelatin and Cellulose Nanofibrils: Biocomposites with Tunable Degradability and Mechanical Behavior

Chondro‐inductive nanofibrous scaffold based gelatin/polyvinyl alcohol/chondroitin sulfate for cartilage tissue engineering

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