A Transcriptional Network Promotes Anthocyanin Biosynthesis in Tomato Flesh

Sun, Chuanlong; Deng, Lisheng; Du, Minmin; Zhao, Jiuhai; Chen, Qian; Huang, Tingting; Jiang, Hongling; Li, Changbao; Li, Chuanyou

doi:10.1016/j.molp.2019.10.010

Cited by 175 publications

(159 citation statements)

References 51 publications

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“…Dietary anthocyanins, which are important health-promoting antioxidants, make major contribution to the quality of fruits or leaves. Furthermore, dietary anthocyanin accumulation is also recognized as a visible biomarker of plants that have suffered from environmental stresses in many studies (Xie Y. et al, 2016;Zheng et al, 2019;Sun et al, 2020). Anthocyanin biosynthesis and regulation pathway has been well studied in Cruciferae plants including Arabidopsis, Brassica rapa, Brassica oleracea, Brassica napus, etc.…”

Section: Introductionmentioning

confidence: 99%

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

et al. 2020

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Brassica juncea is an important dietary vegetable cultivated and consumed in China for its edible stalks and leaves. The purple leaf mustard, which is rich in anthocyanins, is eye-catching and delivers valuable nutrition. However, the molecular mechanism involved in anthocyanin biosynthesis has not been well studied in B. juncea. Here, histological and transcriptome analyses were used to characterize the purple leaf color and gene expression profiles. Free-hand section analysis showed that the anthocyanin was mainly accumulated in the adaxial epidermal leaf cells. The anthocyanin content in the purple leaves was significantly higher than that in the green leaves. To investigate the critical genes and pathways involved in anthocyanin biosynthesis and accumulation, the transcriptome analysis was used to identify the differentially expressed genes (DEGs) between the purple and green leaves from the backcrossed BC3 segregation population in B. juncea. A total of 2,286 different expressed genes were identified between the purple and green leaves. Among them, 1,593 DEGs were up-regulated and 693 DEGs were down-regulated. There were 213 differently expressed transcription factors among them. The MYB and bHLH transcription factors, which may regulate anthocyanin biosynthesis, were up-regulated in the purple leaves. Interestingly, most of the genes involved in plant-pathogen interaction pathway were also up-regulated in the purple leaves. The late biosynthetic genes involved in anthocyanin biosynthesis were highly upregulated in the purple leaves of B. juncea. The up regulation of BjTT8 and BjMYC2 and anthocyanin biosynthetic genes (BjC4H, BjDFR, and BjANS) may activate the purple leaf formation in B. juncea. This study may help to understand the transcriptional regulation of anthocyanin biosynthesis in B. juncea.

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Section: Introductionmentioning

confidence: 99%

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

et al. 2020

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“…Chilling treatment as an effective method to promote dormancy release had been veri ed in many species including tree peony [3][4][5][41][42]. Transcriptomics, proteomics and microRNAs analysis had been performed to investigated the complex mechanism of chilling induced dormancy release in tree peony, which revealed the roles of GA and carbohydrate metabolism [7,13,27].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics analysis reveals Embden Meyerhof Parnas pathway activation and flavonoids accumulation during dormancy transition in tree peony

Zhang

Yuan

Zhan

et al. 2020

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Background: Bud dormancy is a sophisticated strategy which plants evolve to survive in tough environments. Endodormancy is a key obstacle for anti-season culture of tree peony, and sufficient chilling exposure is an effective method to promote dormancy release in perennial plants including tree peony. However, the mechanism of dormancy release is still poorly understood, and there are few systematic studies on the metabolomics during chilling induced dormancy transition.Results: The tree peony buds were treated with artificial chilling, and the metabolmics analysis was employed at five time points after 0-4ºC treatment for 0, 7, 14, 21 and 28 d, respectively. A total of 535 metabolites were obtained and devided into 11 groups including flavonoids, amino acid and its derivatives, lipids, organic acids and its derivates, nucleotide and its derivates, alkaloids, hydroxycinnamoyl derivatives, carbohydrates and alcohols, phytohormones, coumarins and vitamins. Totally, 118 differential metabolites (VIP≥1, P<0.05) during chilling treatment process were detected, and their KEGG pathways involved in several metabolic pathways related to dormancy. Sucrose was the most abundant carbohydrate in peony bud. Starch was degradation and Embden Meyerhof Parnas (EMP) activity were increased during the dormancy release process, according to the variations of sugar contents, related enzyme activities and key genes expression. Flavonoids synthesis and accumulation were also promoted by prolonged chilling. Moreover, the variations of phytohormones (salicylic acid, jasmonic acid, abscisic acid, and indole-3-acetic acid) indicated they played different roles in dormancy transition.Conclusion: Our study suggested that starch degradation, EMP activation, and flavonoids accumulation were crucial and associated with bud dormancy transition in tree peony.

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“…The pathway of avonoid biosynthesis was affected by biotic and abiotic factors (e.g., pathogen infections, temperature, drought, plant hormones) [30]. Moreover, recent reports had shown that avonoids were involved in plant stress response [31], pollen development [32], color formation [33], etc. However, it remained unknown whether avonoids participate in the process of dormancy release.…”

Section: Introductionmentioning

confidence: 99%

Metabonomic analysis reveals EMP pathway activation and flavonoid accumulation during dormancy transition in tree peony

Zhang

Yuan

Zhan

et al. 2020

Preprint

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Background Bud dormancy is a sophisticated strategy in which plants evolved to survive in tough environments. Chilling exposure is an effective method to promote dormancy release in perennial plants including tree peony, and endodormancy is a key obstacle for forcing culture of tree peony. However, the mechanism of dormancy release is still poorly understood, and there are few systematic studies on the metabolomics during chilling induced dormancy transition. Results A total of 535 small molecules were obtained in tree peony buds treated with chilling, belonging to flavonoids, amino acid and its derivatives, lipids, organic acids and its derivates, nucleotide and its derivates, alkaloids, hydroxycinnamoyl derivatives, carbohydrates and alcohols, phytohormones, coumarins, and vitamins, respectively. 118 differential metabolites were detected, and they involved in serveral metabolic pathways related to dormancy. Sucrose was the most abundant carbohydrate in peony bud. Starch degradation and EMP activity were enhanced during the dormancy release, according to the variations of sugar contents, related enzyme activities and key genes expression. Flavonoids synthesis and accunmulation were also promoted by prolonged chilling, which might attribute to the activated expression of PsDFR and PsANS. Moreover, the variations of phytohormones (SA, JA, ABA, and IAA) indicated they played different roles in dormancy transition. Conclusion Our study suggested that starch degradation, EMP activation, and flavonoid accumulation were crucial and associated with bud dormancy transition in tree peony.

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A Transcriptional Network Promotes Anthocyanin Biosynthesis in Tomato Flesh

Cited by 175 publications

References 51 publications

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

Metabolomics analysis reveals Embden Meyerhof Parnas pathway activation and flavonoids accumulation during dormancy transition in tree peony

Metabonomic analysis reveals EMP pathway activation and flavonoid accumulation during dormancy transition in tree peony

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