A transcriptomic analysis of skeletal muscle tissues reveals promising candidate genes and pathways accountable for different daily weight gain in Hanwoo cattle

Sheet, Sunirmal; Jang, Sun Sik; Kim, Jae Hwan; Park, Woncheoul; Kim, Dahye

doi:10.1038/s41598-023-51037-9

Cited by 4 publications

(1 citation statement)

References 66 publications

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“…Skeletal muscle performs numerous biological functions, including voluntary movement, controlling body temperature and regulating metabolism [ 1 , 2 ]. Meanwhile, skeletal muscle, as the main source of animal protein, seriously affects the meat yield of farm animals at harvest [ 3 , 4 ]. During myogenesis, myoblasts derived from the somatic mesoderm undergo a highly coordinated process of myogenic differentiation, culminating in the formation of mature skeletal muscle [ 5 ] and this event requires the concerted action of numerous transcription factors and signaling pathways [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Song,

Zhao,

Zhang

et al. 2024

BMC Genomics

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Background Vitamin A and retinoic acid (RA, a metabolite of vitamin A), are inextricably involved to the development of skeletal muscle in animals. However, the mechanisms regulating skeletal muscle development by vitamin A remain poorly reported. The current study designed to investigate the underlying mechanism of vitamin A affecting myogenic differentiation of lamb myoblasts through transcriptome sequencing (RNA-Seq) and gene function validation experiments. It provides a theoretical basis for elucidating the regulation of vitamin A on skeletal muscle development as well as for improving the economic benefits of the mutton sheep industry. Results Newborn lambs were injected with 7,500 IU vitamin A, and longissimus dorsi (LD) muscle tissue was surgically sampled for RNA-Seq analysis and primary myoblasts isolation at 3 weeks of age. The results showed that a total of 14 down-regulated and 3 up-regulated genes, were identified between control and vitamin A groups. Among them, BHLHE40 expression was upregulated in vitamin A group lambs. Furthermore, BHLHE40 expression is significantly increased after initiation of differentiation in myoblasts, and RA addition during differentiation greatly promoted BHLHE40 mRNA expression. In vitro, RA inhibited myoblasts proliferation and promoted myoblasts myogenic differentiation through BHLHE40. Moreover, BHLHE40 was proved to inhibit the expression of the DNA binding inhibitor 3 (ID3), and meanwhile, ID3 could effectively promote myoblasts proliferation and inhibit myoblasts myogenic differentiation. Conclusions Taken together, our results suggested that vitamin A inhibited myoblasts proliferation and promoted myoblasts myogenic differentiation by inhibiting ID3 expression through BHLHE40.

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Section: Introductionmentioning

confidence: 99%

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Song,

Zhao,

Zhang

et al. 2024

BMC Genomics

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Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle

Sheet,

Jang,

Lim

et al. 2024

Animal Biotechnology

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Identification of Heilongjiang crossbred beef cattle pedigrees and reveals functional genes related to economic traits based on whole-genome SNP data

Li,

Liu,

Ahmed

et al. 2024

Front. Genet.

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Introduction: To enhance the beef cattle industry, Heilongjiang Province has developed a new Crossbred beef cattle variety through crossbreeding with exotic commercial breeds. This new variety exhibits relatively excellent meat quality, and efficient reproductive performance, catering to market demands.Method: This study employed whole genome resequencing technology to analyze the genetic pedigree and diversity of 19 Heilongjiang Crossbred beef cattle, alongside 59 published genomes from East Asian, Eurasian, and European taurine cattle as controls. In addition, genes related to production traits were also searched by identifying Runs of Homozygosity (ROH) islands and important fragments from ancestors.Results: A total of 14,427,729 biallelic SNPs were discovered, with the majority located in intergenic and intron regions and a small percentage in exon regions, impacting protein function. Population genetic analyses including Principal Component Analysis (PCA), Neighbor-Joining (NJ) tree, and ADMIXTURE identified Angus, Holstein, and Mishima as the main ancestors of Crossbred beef cattle. In genetic diversity analysis, nucleotide diversity, linkage disequilibrium, and inbreeding coefficient analysis reveal that the genetic diversity of Crossbred beef cattle is at a moderate level, and a higher inbreeding coefficient indicates the need for careful breeding management. In addition, some genes related to economic traits are identified through the identification of Runs of Homozygosity (ROH) islands and important fragments from ancestors.Conclusion: This comprehensive genomic characterization supports the targeted improvement of economically important traits in Crossbred beef cattle, facilitating advanced breeding strategies.

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A transcriptomic analysis of skeletal muscle tissues reveals promising candidate genes and pathways accountable for different daily weight gain in Hanwoo cattle

Cited by 4 publications

References 66 publications

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle

Identification of Heilongjiang crossbred beef cattle pedigrees and reveals functional genes related to economic traits based on whole-genome SNP data

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