A Transgenic Model for Listeriosis: Role of Internalin in Crossing the Intestinal Barrier

Lecuit, Marc; Vandormael-Pournin, Sandrine; Lefort, Jean; Huerre, Michel; Gounon, Pierre; Dupuy, Catherine; Babinet, Charles; Cossart, Pascale

doi:10.1126/science.1059852

Cited by 566 publications

(567 citation statements)

References 19 publications

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“…Interestingly, L. monocytogenes internalin does not bind mouse E-cadherin, a result that may explain why orally infected mice are only marginally susceptible to L. monocytogenes infection. In agreement with this hypothesis, transgenic mice in which human E-cadherin was selectively expressed in enterocytes exhibited both an increased susceptibility to systemic bacterial infection and differences in the type of cells that were infected as compared with wild-type mice [22]. Because of this species-specific difference between humans and mice, most investigators who have studied the immune response against L. monocytogenes in mice have used the intravenous route of infection.…”

Section: The Importance Of the Route And Dose Of Infectionmentioning

confidence: 77%

Mini‐review: Presentation of pathogen‐derived antigens in vivo

Lauvau

Glaichenhaus

2004

Eur J Immunol

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Most intracellular pathogens induce robust T cell responses upon infection of mammalian hosts. In most cases, these T cell responses are protective and result in pathogen clearance. It is therefore important to determine how T cells are primed and how they differentiate into cytokine-secreting and/or cytotoxic effector cells. In contrast to B cells, which recognize soluble Ag, CD8 + and CD4 + T cells react to Ag-derived peptides bound to MHC I or MHC II molecules, respectively. Therefore, elucidating the mechanisms by which pathogen-derived Ag become available for presentation is necessary to understand how pathogens trigger T cell responses in vivo. Although many excellent reviews have focused on the mechanisms involved in Ag processing, very few have pointed to the specificity of host-pathogen interactions. In this respect, it should be noticed that these interactions are very different from one pathogen to another, and may result in the involvement of different cells and molecules. Because of space limitations, we have decided to focus this review on two intracellular pathogens -vaccinia virus and Listeria monocytogenes. We have chosen these two pathogens because they both induce a strong CD8 + T cell response and because they have been extensively studied by both microbiologists and immunologists.

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Section: The Importance Of the Route And Dose Of Infectionmentioning

confidence: 77%

Mini‐review: Presentation of pathogen‐derived antigens in vivo

Lauvau

Glaichenhaus

2004

Eur J Immunol

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“…Humans are mainly infected with L. monocytogenes via the gastrointestinal tract (foodborne disease) while in mice, enteral infection is not efficient due to a single amino acid substitution in murine E-cadherin (11,12). Healthy humans usually clear L. monocytogenes infection with little or no clinical symptoms.…”

Section: Introductionmentioning

confidence: 99%

Indoleamine 2,3-dioxygenase–expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection

Попов¹,

Abdullah²,

et al. 2006

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Control of pathogens by formation of abscesses and granulomas is a major strategy of the innate immune system, especially when effector mechanisms of adaptive immunity are insufficient. We show in human listeriosis that DCs expressing indoleamine 2,3-dioxygenase (IDO), together with macrophages, are major cellular components of suppurative granulomas in vivo. Induction of IDO by DCs is a cell-autonomous response to Listeria monocytogenes infection and was also observed in other granulomatous infections with intracellular bacteria, such as Bartonella henselae. Reporting on our use of the clinically applied anti-TNF-α antibody infliximab, we further demonstrate in vitro that IDO induction is TNF-α dependent. Repression of IDO therefore might result in exacerbation of granulomatous diseases observed during anti-TNF-α therapy. These findings place IDO + DCs not only at the intersection of innate and adaptive immunity but also at the forefront of bacterial containment in granulomatous infections.

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“…Importantly, Lmdd-gag was attenuated at least 5 logs in neonatal mice [35]. However, mice are not optimal for testing the safety and efficacy of Lmdd-gag as they are neither susceptible to lentivirus infection nor encode the proper E-cadherin receptor on intestinal epithelial cells to allow Lm to enter enterocytes [36].…”

Section: Introductionmentioning

confidence: 99%

Live attenuated Listeria monocytogenes expressing HIV Gag: Immunogenicity in rhesus monkeys

Jiang

Rasmussen

Nolan

et al. 2007

Vaccine

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Induction of strong cellular immunity will be important for AIDS vaccine candidates. Natural infection with wild-type Listeria monocytogenes (Lm), an orally transmitted organism, is known to generate strong cellular immunity, thus raising the possibility that live attenuated Lm could serve as a vaccine vector. We sought to examine the potential of live attenuated Lm to induce cellular immune responses to HIV Gag. Rhesus macaques were immunized with Lmdd-gag that expresses HIV gag and lacks two genes in the D-alanine (D-ala) synthesis pathway. Without this key component of the bacterial cell wall, vaccine vector replication critically depends on exogenous D-ala. Lmdd-gag was given to animals either solely orally or by oral priming followed by intramuscular (i.m.) boosting; D-ala was co-administered with all vaccinations. Lmdd-gag and D-ala were well tolerated. Oral priming/oral boosting induced Gag-specific cellular immune responses, whereas oral priming/i.m. boosting induced systemic as well as mucosal anti-Gag antibodies. These results suggest that the route of vaccination may bias anti-Gag immune responses either towards T-helper type 1 (Th1) or Th2 responses; overall, our data show that live attenuated, recombinant Lmdd-gag was safe and immunogenic in primates.

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A Transgenic Model for Listeriosis: Role of Internalin in Crossing the Intestinal Barrier

Cited by 566 publications

References 19 publications

Mini‐review: Presentation of pathogen‐derived antigens in vivo

Mini‐review: Presentation of pathogen‐derived antigens in vivo

Indoleamine 2,3-dioxygenase–expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection

Live attenuated Listeria monocytogenes expressing HIV Gag: Immunogenicity in rhesus monkeys

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