A transgenic silkworm overexpressing human lysosomal enzyme as a novel resource for producing recombinant glycobiologics and its application to development of enzyme replacement therapy for lysosomal diseases

Itoh, Kohji; Kobayashi, Isao; Nishioka, So-ichiro; Hidaka, Tomo; Tsuji, Daisuke; Sezutsu, Hideki

doi:10.1016/j.ymgme.2016.11.161

Cited by 1 publication

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“…Historically, B. mori gained prominence due to silk production, but now it is one of the most valued model organisms for life science branches. Just to mention a few recent achievements, B. mori has been used as a bioreactor, for recombinant proteins production [7][8][9][10], while its silk is used to produce extraordinary silk-based biomaterials that exhibit great importance for the medical field [11]. The silk proteome contains two major proteins, namely fibroin and sericin.…”

Section: Introductionmentioning

confidence: 99%

Advances in Editing Silkworms (Bombyx mori) Genome by Using the CRISPR-Cas System

Baci

Cucu

Giurgiu

et al. 2021

Insects

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CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) represents a powerful genome editing technology that revolutionized in a short period of time numerous natural sciences branches. Therefore, extraordinary progress was made in various fields, such as entomology or biotechnology. Bombyx mori is one of the most important insects, not only for the sericulture industry, but for numerous scientific areas. The silkworms play a key role as a model organism, but also as a bioreactor for the recombinant protein production. Nowadays, the CRISPR-Cas genome editing system is frequently used in order to perform gene analyses, to increase the resistance against certain pathogens or as an imaging tool in B. mori. Here, we provide an overview of various studies that made use of CRISPR-Cas for B. mori genome editing, with a focus on emphasizing the high applicability of this system in entomology and biological sciences.

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