A Transition Metal Carbonyl Probe for Use in a Highly Specific and Sensitive SERS-Based Assay for Glucose

Abstract

A triosmium carbonyl cluster-boronic acid conjugate is used as a secondary carbohydrate probe in a SERS-based assay. The assay does not require conjugation of the metal carbonyl probe to a SERS-active species, and it utilizes the CO stretching vibrations of the metal carbonyl, which lies in a silent region of the SERS spectrum (1800-2200 cm(-1)), for quantification. High selectivity for glucose over fructose and galactose is obtained, and a human urine sample doped with glucose is detected accurately.

Highly selective and sensitive surface enhanced Raman scattering nanosensors for detection of hydrogen peroxide in living cells

“…1B), meaning that 4-CA molecules were successfully functionalized on Au NPs by amide bonds since SERS is a surface selective technique and Au NPs have been washed with ultrapure water. In the SERS spectrum, the strong Raman bands at 821, 1011, 1031, 1178, 1270, 1562 cm À 1 (Table S1) (Piergies et al, 2013;Alver and Parlak, 2010;Kong et al, 2013). It also can be seen from Fig.…”

Glucose Sensing in Supramolecular Chemistry

“…The boronic acid served as capturing agent, and the triosmium carbonylboronic acid was employed as a secondary label (in a sandwich assay). 174 A recent multicolorimetric sensor array was developed that used boronic acid-based thin films combined with three anionic dyes for colorimetric saccharide detection. 175 Hindsgaul and co-workers 91 have developed a simple nakedeye method to determine terminal glycosylation of a glycoprotein (Scheme 27).…”

Clustering Small Dendrimers into Nanoaggregates for Efficient DNA and siRNA Delivery with Minimal Toxicity

“…[ 29,31 ] The specifi c bindings between PBA and diols are widely used to construct micelles, hydrogels, and biosensors. [32][33][34] In addition, PBA is capable of forming reversible covalent esters with the ribose ring of DNA or siRNA, thus cationic polymers modifi ed with PBA can bind with nucleic acid via the boronic ester linkage except ionic interactions. [ 35 ] PBA containing polymers are also used as gene vectors based on these promising properties.…”