A translational approach to assess the metabolomic impact of stabilized gold nanoparticles by NMR spectroscopy

Abstract

The metabolomic impact of gold nanoparticles on a cell model can be modulated by a stabilizer.

A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes

“…A representative spectrum of RBC extracts is shown in Figure 1 , where several metabolites could be identified and quantified, based on previous data [ 56 , 57 ] and 2D-NMR spectra ( Supplementary Table S1 ).…”

A Metabolomic Approach for the In Vivo Study of Gold Nanospheres and Nanostars after a Single-Dose Intravenous Administration to Wistar Rats

“…In fact, the concentration of AuNPs used in our study was lower than in many vivo studies [11,12,38], so no significant differences are expected in the conventional toxicological assays. As metabolomics showed the ability to detect modifications in various biological pathways even at low subtoxic concentration of AuNPs [39], further discussion will mainly focus on this approach.…”

Alteration of the Mitochondrial Effects of Ceria Nanoparticles by Gold: An Approach for the Mitochondrial Modulation of Cells Based on Nanomedicine

“…Once the NPs had been successfully obtained, their biocompatibility was evaluated using HeLa cells, in which cell viability and proliferation were assessed. These studies were performed using CeO 2 , AuCeO 2 and TPP-AuCeO 2 at two usual concentrations employed in biomedicine (i.e., 10 and 20 µg/mL) [16]. The cytotoxicity of these NPs in HeLa cells was determined by MTT assay at 24, 48 and 72 h of incubation.…”

“…CeO 2 is a well-established antioxidant agent in biomedicine [10,11]. The antioxidant properties of CeO 2 can be enhanced when it is combined with certain metals such as gold, silver or platinum [12][13][14][15][16], which have been proposed as biocompatible materials acting as radical scavengers and catalase-like enzymes to treat oxidative stress (OS) [14,[17][18][19][20]. Furthermore, it has been suggested that CeO 2 NPs, alone or in combination with metals, protect against radiation and have beneficial effects on different pathologies such as dementia and infection [21][22][23][24][25].…”

“…Diseases with OS needed to be treated with specific antioxidants, taking into account their cellular action mechanism, mainly by determining the influence on the mitochondria function as the main ROS source. Although widespread, the use of antioxidants to prevent oxidative stress can often yield inconclusive or contradictory results due to insufficient understanding of their mode of action and their effect on mitochondrial function [16,34,35]. This is the case with several NPs that have been proposed as antioxidants in preclinical studies, for which a lack of knowledge regarding their mitochondrial action mechanism has inevitably hampered their clinical translation [36].…”