A Trehalose Quantitative Sensor Based on Ni Foam Material Modified with Graphene Oxidated and Non-linear Analysis Model

Fang, Xudong; Mei, Zhenghao; Chen, Jiaqi; Xiong, Siyi; Ning, Jingyuan; Jiang, Chenhao; Gao, Yuanyuan; Yi, Xiaomei; Hui, Guohua

doi:10.1007/s12161-021-02028-x

Cited by 8 publications

(2 citation statements)

References 18 publications

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“…Then, qPCR was performed with Bestar®SybrGreen qPCR mastermix (DBI). Relative gene expressions were determined by 2 −ΔΔCt method, with circPUM1, JUNB and miR-30a-5p expression respectively normalized to GAPDH or U6 [15][16][17][18]. The primer sequences applied were as listed below: circPUM1 Forward: 5′-GATTATTCAGGCACGCAGGT-3′, Reverse: 5′-CCCTCCTCCTTCAAATCTCC-3′; JUNB Forward: 5′-GACACAGGCGCATCTCTGAAG -3ʹ,Reverse: 5′-GATCACGCCGTTGCTGTTG-3ʹ; GAPDH Forward: 5′-GCACCGTCAAGGCTGAGAAC-3′, Reverse: 5′-ATGGTGGTGAAGACGCCAGT-3ʹ; miR-30a-5p Forward: CGCGATGTTGAAACATCCTCGAC-3ʹ, Reverse: 5ʹ-ATCCAGTGCAGGGTCCGAGG -3ʹ; U6 Forward: 5′-CCTGCGCAAGGATGAC -3′, Reverse: 5′--GCTTCGGCAGCACATATACTAAAAT-3′.…”

Section: Rt-qpcrmentioning

confidence: 99%

Circular RNA PUM1 (CircPUM1) attenuates trophoblast cell dysfunction and inflammation in recurrent spontaneous abortion via the MicroRNA-30a-5p (miR-30a-5p)/JUNB axis

Zhu

Shi

et al. 2021

Bioengineered

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Recurrent spontaneous abortion (RSA) is a threat to human reproductive health worldwide. CircPUM1 has been reported to participate in the pathogenesis of various diseases. However, there has been no report on its association with RSA yet. In this study, gene expressions were examined by RT-qPCR. Protein levels of JUNB and cleaved caspases-3 were detected by Western blotting. ELISA was used to detect TNF-α, IL-6, and IL-8 levels. Cell viability, migration, invasion, and apoapsis were analyzed using CCK-8, transwell, and flow cytometry assays. The association between miR-30a-5p and circPUM1 or JUNB was identified by bioinformatics analysis, dualluciferase reporter assay, and RIP assay. Herein, we found circPUM1 was significantly downregulated in RSA placental samples. CircPUM1 knockdown induced decreased proliferation, migration, and invasion, but increased apoptosis, pro-apoptotic protein (cleaved caspases-3) level, and proinflammatory factor (TNF-α, IL-6, and IL-8) secretion in trophoblast cells. Furthermore, we confirmed that circPUM1 was a sponge for miR-30a-5p, and JUNB was directly targeted by miR-30a-5p. It was demonstrated that miR-30a-5p inhibition could reverse trophoblast cell dysfunction and inflammation induced by circPUM1 knockdown. In addition, we found that JUNB expression was negatively modulated by miR-30a-5p and positively regulated by circPUM1. Moreover, circPUM1 inhibition exacerbated dysfunction and inflammation in trophoblast cells via targeting JUNB. To sum up, our study indicated that circPUM1 could impair RSA occurrence and development by facilitating trophoblast cellular processes and protecting against inflammation via the miR-30a-5p/JUNB axis, providing a new target for the improvement of RSA diagnosis and treatment.

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Section: Rt-qpcrmentioning

confidence: 99%

Circular RNA PUM1 (CircPUM1) attenuates trophoblast cell dysfunction and inflammation in recurrent spontaneous abortion via the MicroRNA-30a-5p (miR-30a-5p)/JUNB axis

Zhu

Shi

et al. 2021

Bioengineered

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“…where A is signal intensity, and f is signal frequency.D is external noise intensity. SR is qualified by SNR index and SNR can be approximately described as [19][20][21] SNR ¼…”

Section: Nonlinear Stochastic Resonancementioning

confidence: 99%

Rapid quantitative detection of glucose using biological sensor system as combined with electrochemical data treatment

Wan

Zhao

Huang

et al. 2021

International Journal of Food Properties

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In this paper, glucose quantitative detection by biological sensor and electrochemical capacitance data treatment was studied. The taste cells were fixed on carbon screen-printed electrode. Electrochemical capacitance responses of cell-based sensor to sucrose in different concentrations and related mixtures were measured. Glucose quantitative analysis results demonstrated that the proposed method had quantitative determination abilities toward glucose and related mixtures. The signal-to-noise ratio index presented qualitative discrimination abilities to different glucose mixtures. Negative control results indicated that the receptor-free cells showed no perception ability for glucose. The proposed method is promising in sugar rapid detection.

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Effects of electrocatalytic treatment on the physicochemical properties of rice bran protein

Wang

et al. 2021

Food Measure

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A Trehalose Quantitative Sensor Based on Ni Foam Material Modified with Graphene Oxidated and Non-linear Analysis Model

Cited by 8 publications

References 18 publications

Circular RNA PUM1 (CircPUM1) attenuates trophoblast cell dysfunction and inflammation in recurrent spontaneous abortion via the MicroRNA-30a-5p (miR-30a-5p)/JUNB axis

Circular RNA PUM1 (CircPUM1) attenuates trophoblast cell dysfunction and inflammation in recurrent spontaneous abortion via the MicroRNA-30a-5p (miR-30a-5p)/JUNB axis

Rapid quantitative detection of glucose using biological sensor system as combined with electrochemical data treatment

Effects of electrocatalytic treatment on the physicochemical properties of rice bran protein

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