A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions

Li, Pengfei; Wang, Shuangchao; Zhang, Lihang; Zhou, Xueping; Guo, Lihua

doi:10.1126/sciadv.aay9634

Cited by 79 publications

(66 citation statements)

References 56 publications

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“…Nevertheless, many mycoviruses remain unclassified [ 13 ]. Besides RNA viruses, a few ssDNA viruses have been reported, such as Sclerotinia sclerotiorum [ 14 ] and Fusarium graminearum [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Multiple mycoviruses identified in Pestalotiopsis spp. from Chinese bayberry

Chen

Wang

et al. 2021

Virol J

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Background Chinese bayberry (Myrica rubra) is a subtropical fruit crop widely grown in southern China. Twig dieback is a disease of Chinese bayberry caused by Pestalotiopsis spp. and results in great economic losses to Chinese bayberry production. A virus survey was conducted in the population of Pestalotiopsis spp. infecting M. rubra in China. We explored the viral diversity in Pestalotiopsis spp., which may provide resources for further development as biocontrol agents of twig dieback. Methods Strains of Pestalotiopsis spp. were isolated from diseased twigs of M. rubra, and cultured on potato dextrose agar for RNA extraction. The total RNA of each strain was extracted, mixed, and used for RNA sequencing. The resulting sequences were deduplicated, annotated, and then used for phylogenetic analysis. Results Seven novel viruses were characterized from 59 isolates of M. rubra collected from 14 localities in China. Based on the phylogenetic analysis, these viruses were classified into five viral families/orders, Botourmiaviridae, Mitoviridae, Partitiviridae, Tymovirales and Bunyavirales, and one virus, Pestalotiopsis negative-stranded RNA virus 1, which likely belongs to a new viral family. Conclusions Metatranscriptomics analysis showed the presence of various mycoviruses in Pestalotiopsis spp. isolated from M. rubra in China. The genomes of eight putative viruses were identified, seven of which were nearly full-length. Some of these viruses of Pestalotiopsis spp. may have the potential for the biological control of twig dieback of M. rubra.

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Section: Introductionmentioning

confidence: 99%

Multiple mycoviruses identified in Pestalotiopsis spp. from Chinese bayberry

Chen

Wang

et al. 2021

Virol J

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“…Mycoviruses have their genomes of double-stranded (ds) RNA [3], circular single-stranded (ss) DNA [14,15], negative-sense single-stranded (-ss) RNA [16], positive-sense single-stranded (+ ss) RNA linear genomes and reverse-transcribing RNA linear genomes [3]. Viruses in the family Partitiviridae comprised of two dsRNA segments, in sizes ranging from 1,300 bp to 2,500 bp, putatively encoding proteins of RNA-dependent RNA polymerase (RdRp) and coat protein (CP), respectively.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of a Novel Partitivirus and a Fusarivirus Co-infected in the Nigrospora Sphaerica Fungus

Zhong

Yang

et al. 2021

Preprint

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Here we reported the molecular characterization of two novel mycoviruses co-infected in a plant pathogenic fungus, Nigrospora sphaerica that were designated as Nigrospora sphaerica fusarivirus 1 (NsFV1) and Nigrospora sphaerica partitivirus 1 (NsPV1), respectively. NsFV1 has an undivided genome of 6,147 bp, excluding the polyA tail, and was predicted to contain two nonoverlapping open reading frames (ORF1 and 2). The larger ORF1 encoded a polyprotein containing a conserved RNA-dependent RNA polymerase (RdRp) and a helicase domain that have functions for RNA replication, and the smaller ORF2 encoded a putative protein with an unknown function. The NsPV1 was consists of two genome segments, which were in lengths of 1,796 bp and 1,455 bp, respectively. Each of the two dsRNAs had a single ORF and were deduced to encode proteins with homology to viral RdRp and coat protein (CP), respectively, in the family Partitiviridae. Phylogenetic analysis showed that NsFV1 was placed within the newly proposed family Fusariviridae, while NsPV1 was belonging to the genus Gammapartitivirus in the family Partitiviridae. This was the first description of mycovirses infected the fungus N. sphaerica.

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“…2014 ; Donaire, Pagán, and Ayllón 2016 ; Varsani and Krupovic 2017 ; Wang et al. 2018 ; Li et al. 2020 ).…”

Section: Introductionunclassified

Divergent RNA viruses in Macrophomina phaseolina exhibit potential as virocontrol agents

wang

Liu

et al. 2020

Virus Evolution

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Macrophomina phaseolina is an important necrotrophic phytopathogenic fungus and cause extensive damage in many oilseed crops. Twelve M.phaseolina isolates with diverse biological phenotypes were selected for a high-throughput sequencing-based metatranscriptomic and bioinformatics analysis to identify viruses infecting M.phaseolina . The analysis identified 40 partial or nearly complete viral genome segments, 31 of which were novel viruses. Among these viral sequences, 43% of the viral genomes were double-stranded RNA (dsRNA), 47% were positive single-stranded RNA (ssRNA+), and the remaining 10% were negative sense-stranded RNA (ssRNA−). The 40 viruses showed affinity to 13 distinct viral lineages, including Bunyavirales (four viruses), Totiviridae (three viruses), Chrysoviridae (five viruses), Partitiviridae (four viruses), Hypoviridae (one virus), Endornaviridae (two viruses), Tombusviridae (three viruses), Narnaviridae (one virus), Potyviridae (one virus), Bromoviridae (one virus), Virgaviridae (six viruses), ‘Fusagraviridae’ (five viruses), and Ourmiavirus (four viruses). Two viruses are closely related to two families, Potyviridae and Bromoviridae , which previously contained no mycovirus species. Moreover, nine novel viruses associated with M.phaseolina were identified in the family Totiviridae , Endornaviridae , and Partitiviridae . Coinfection with multiple viruses is prevalent in M.phaseolina , with each isolate harboring different numbers of viruses, ranging from three to eighteen. Furthermore, the effects of the viruses on the fungal host were analyzed according to the biological characteristics of each isolate. The results suggested that M.phaseolina hypovirus 2, M.phaseolina fusagravirus virus 1-5 (MpFV1-5), M.phaseolina endornavirus 1-2 (MpEV1-2), M.phaseolina ourmia-like virus 1-3 (MpOLV1-3), M.phaseolina mitovirus 4 (MpMV4), and M.phaseolina mycobunyavirus 1-4 (MpMBV1-4) were only detected in hypovirulent isolates. Those viruses associated with hypovirulence might be used as biological control agents as an environmentally friendly alternative to chemical fungicides. These findings considerably expand our understanding of mycoviruses in M.phaseolina and unvailed the presence of a huge difference among viruses in isolates from different hosts in distant geographical regions. Together, the present study provides new knowledge about...

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A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions

Cited by 79 publications

References 56 publications

Multiple mycoviruses identified in Pestalotiopsis spp. from Chinese bayberry

Multiple mycoviruses identified in Pestalotiopsis spp. from Chinese bayberry

Molecular Characterization of a Novel Partitivirus and a Fusarivirus Co-infected in the Nigrospora Sphaerica Fungus

Divergent RNA viruses in Macrophomina phaseolina exhibit potential as virocontrol agents

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