A triple amplification strategy using GR-5 DNAzyme as a signal medium for ultrasensitive detection of trace Pb2+ based on CRISPR/Cas12a empowered electrochemical biosensor

Yue, Yuanyuan; Wang, Songtao; Jin, Qiang; An, Nan; Wu, Liangpeng; Huang, He

doi:10.1016/j.aca.2023.341241

Cited by 10 publications

(1 citation statement)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The exceptional sensitivity achieved in this method can be attributed to a triple-cycle amplification process, including the dual cycle formed by SDA itself and the inherent signal-enhancing properties of CRISPR/Cas12a. As a result, the SM-E-CRISPR detection platform achieves a sensitivity level in the picomolar (pM) range [74]. This provides a straightforward, highly sensitive, and real-time method for detecting various other heavy metal ions.…”

Section: Crispr/cas Amplification Strategymentioning

confidence: 99%

Engineered Electrochemiluminescence Biosensors for Monitoring Heavy Metal Ions: Current Status and Prospects

Chen,

Jiang,

Liu

et al. 2023

Biosensors

View full text Add to dashboard Cite

Metal ion contamination has serious impacts on environmental and biological health, so it is crucial to effectively monitor the levels of these metal ions. With the continuous progression of optoelectronic nanotechnology and biometrics, the emerging electrochemiluminescence (ECL) biosensing technology has not only proven its simplicity, but also showcased its utility and remarkable sensitivity in engineered monitoring of residual heavy metal contaminants. This comprehensive review begins by introducing the composition, advantages, and detection principles of ECL biosensors, and delving into the engineered aspects. Furthermore, it explores two signal amplification methods: biometric element-based strategies (e.g., HCR, RCA, EDC, and CRISPR/Cas) and nanomaterial (NM)-based amplification, including quantum dots, metal nanoclusters, carbon-based nanomaterials, and porous nanomaterials. Ultimately, this review envisions future research trends and engineered technological enhancements of ECL biosensors to meet the surging demand for metal ion monitoring.

show abstract

Section: Crispr/cas Amplification Strategymentioning

confidence: 99%