A TRP homolog in
            <i>Saccharomyces cerevisiae</i>
            forms an intracellular Ca
            <sup>2+</sup>
            -permeable channel in the yeast vacuolar membrane

Palmer, Chris P.; Zhou, Xian; Lin, J. G.; Loukin, Stephen H.; Kung, Ching; Saimi, Yoshiro

doi:10.1073/pnas.141036198

Cited by 216 publications

(238 citation statements)

References 42 publications

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“…YVC1 (wild-type parental strain): BY4742 (MAT␣ his3⌬1 leu2⌬ lys2⌬ ura3⌬), and yvc1⌬ (YVC1 chromosomalknockout strain; YVC1:km): BY4742 containing a complete chromosomal replacement of the entire YVC1 with a kanamycin resistance gene (26).…”

Section: Methodsmentioning

confidence: 99%

“…pYVC1-His was generated by PCR from YVC1-HA (26), attaching an XmaI site to the 5Ј end of the YVC1 coding sequence and a 10-His tag, the stop codon, and an XhoI site to the 3Ј end. The 5Ј primer was as follows: 5Ј-TCC CCC GGG ATG GTA TCA GCC AAC GGC GAC TTG CAC TTG-3Ј.…”

Section: Methodsmentioning

confidence: 99%

“…5) became a single spherical vacuole. The spheroplasts with their individual vacuoles were then swollen by hypoosmolarity to shed their plasma membranes so as to expose the vacuoles for patch-clamping as reported (26). The pipet solution contained 180 mM KCl and 0.1 mM EGTA and the bath solution contained 100 mM potassium citrate.…”

Section: Methodsmentioning

confidence: 99%

“…An Ϸ400-pS cation conductance has repeatedly been observed (27,28,31) that is activated by Ca 2ϩ presented from the cytoplasmic side. We cloned the TRP homolog YVC1 of yeast and showed that it is required for this conductance (26). It had been tacitly assumed that the isolated vacuoles were too fragile for direct mechanical examination.…”

Section: Yvc1p Is Msmentioning

confidence: 99%

“…At the same time, Ca 2ϩ is released from the vacuole into the cytoplasm (25). We cloned and examined Yvc1p, an Ϸ400-pS Trp-like Ca 2ϩ -permeable channel (26), which was found to be the conduit for this upshock-induced Ca 2ϩ release (25). Despite the fragility of isolated vacuoles, we have examined the effect of mechanical and osmotic pressure on them and discovered that Yvc1p is in fact MS.…”

mentioning

confidence: 99%

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The transient receptor potential channel on the yeast vacuole is mechanosensitive

Zhou

Batiza²,

Loukin³

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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147

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Ca 2؉ is released from the vacuole into the yeast cytoplasm on an osmotic upshock, but how this upshock is perceived was unknown. We found the vacuolar channel, Yvc1p, to be mechanosensitive, showing that the Ca 2؉ conduit is also the sensing molecule. Although fragile, the yeast vacuole allows limited direct mechanical examination. Pressures at tens of millimeters of Hg (1 mmHg ‫؍‬ 133 Pa) activate the 400-pS Yvc1p conductance in whole-vacuole recording mode as well as in the excised cytoplasmic-side-out mode. Raising the bath osmolarity activates this channel and causes vacuolar shrinkage and deformation. It appears that, on upshock, a transient osmotic force activates Yvc1p to release Ca 2؉ from the vacuole. Mechanical activation of Yvc1p occurs regardless of Ca 2؉ concentration and is apparently independent of its known Ca 2؉ activation, which we now propose to be an amplification mechanism (Ca 2؉ -induced Ca 2؉ release). Yvc1p is a member of the transient receptor potential-family channels, several of which have been associated with mechanosensation in animals. The possible use of Yvc1p as a molecular model to study mechanosensation in general is discussed.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Yvc1p Is Msmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The transient receptor potential channel on the yeast vacuole is mechanosensitive

Zhou

Batiza²,

Loukin³

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

147

123

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Calcium‐dependent antimicrobials: Nature‐inspired materials and designs

Wang,

Zeng,

Ahmed

et al. 2024

Exploration

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Bacterial infection remains a major complication answering for the failures of various implantable medical devices. Tremendous extraordinary advances have been published in the design and synthesis of antimicrobial materials addressing this issue; however, the clinical translation has largely been blocked due to the challenge of balancing the efficacy and safety of these materials. Here, calcium's biochemical features, natural roles in pathogens and the immune systems, and advanced uses in infection medications are illuminated, showing calcium is a promising target for developing implantable devices with less infection tendency. The paper gives a historical overview of biomedical uses of calcium and summarizes calcium's merits in coordination, hydration, ionization, and stereochemistry for acting as a structural former or trigger in biological systems. It focuses on the involvement of calcium in pathogens’ integrity, motility, and metabolism maintenance, outlining the potential antimicrobial targets for calcium. It addresses calcium's uses in the immune systems that the authors can learn from for antimicrobial synthesis. Additionally, the advances in calcium's uses in infection medications are highlighted to sketch the future directions for developing implantable antimicrobial materials. In conclusion, calcium is at the nexus of antimicrobial defense, and future works on taking advantage of calcium in antimicrobial developments are promising in clinical translation.

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Comparative transcriptome analysis of the calcium signaling and expression analysis of sodium/calcium exchanger in Aspergillus cristatus

et al. 2017

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Aspergillus cristatus develops into various stages under different Na concentrations:the sexual stage in 0.5 M NaCl and asexual development stage in 3 M NaCl. In order to explore whether the Ca 2+ signaling pathway in A. cristatus responded to the changes in the salt stress, we analyzed the gene expression levels in A. cristatus respectively cultured in 0.5 M NaCl and 3 M NaCl. According to the BLAST analysis results, we identified 25 Ca 2+ -signaling proteins in A. cristatus. The expression levels of most genes involved in the Ca 2+ -signaling pathway in A. cristatus cultured in different salt concentrations showed significant differences, indicating that the Ca 2+ signaling pathway was involved in the response to the changes in the salt stress. In yeasts, only calcium ion influx proteins were reported to be involved in the response to the changes in the salt stress. So far, the protein for the exchanger of calcium/sodium ions has not been reported. Therefore, we obtained the sodium/calcium exchanger (termed NCX) proteins from the KEGG Database. The ncx gene of A. cristatus was cloned and characterized. The full length of ncx gene is 3055 bp, including a 2994-bp open reading frame encoding 994 amino acids. The expression levels of ncx in the sexual development stage and asexual development stage were respectively ∼8.94 times and ∼2.57 times of that in the hyphal formation stage. Therefore, we suggested that ncx gene was up-regulated to resist the sodium stress. The study results provide the basis for further exploring the Ca 2+ -signaling mechanism and ion exchanger mechanism.

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A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca ²⁺ -permeable channel in the yeast vacuolar membrane

Cited by 216 publications

References 42 publications

The transient receptor potential channel on the yeast vacuole is mechanosensitive

The transient receptor potential channel on the yeast vacuole is mechanosensitive

Calcium‐dependent antimicrobials: Nature‐inspired materials and designs

Comparative transcriptome analysis of the calcium signaling and expression analysis of sodium/calcium exchanger in Aspergillus cristatus

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A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca 2+ -permeable channel in the yeast vacuolar membrane

Cited by 216 publications

References 42 publications

The transient receptor potential channel on the yeast vacuole is mechanosensitive

The transient receptor potential channel on the yeast vacuole is mechanosensitive

Calcium‐dependent antimicrobials: Nature‐inspired materials and designs

Comparative transcriptome analysis of the calcium signaling and expression analysis of sodium/calcium exchanger in Aspergillus cristatus

Contact Info

Product

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A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca ²⁺ -permeable channel in the yeast vacuolar membrane