A trypsin inhibitor cDNA from a novel source, snail medic (Medicago scutellata L.): cloning and functional expression in response to wounding, herbivore, jasmonic and salicylic acid

Balestrazzi, Alma; Confalonieri, Massimo; Odoardi, M.; Ressegotti, Valentina; Allegro, Gianni; Tava, Aldo; Carbonera, Daniela

doi:10.1016/j.plantsci.2004.04.002

Cited by 10 publications

(6 citation statements)

References 45 publications

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“…The amino acid sequence of AATI showed high similarity with the BBIs from Glycine max (72%), Medicago sativa (72%), Medicago scutellata (68%), Pisum sativum (72%), and Phaseolus vulgaris (68%). [31][32][33][34] The positions of 14 cysteine residues are conserved, and two reactive sites of AATI were deduced to be Lys62 and Arg88. These data suggest that AATI belongs to the BBI family of inhibitors.…”

Section: Cloning Of Aati Cdna and Sequence Analysismentioning

confidence: 99%

Purification, Characterization, and cDNA Cloning of a Bowman-Birk Type Trypsin Inhibitor fromApios americanaMedikus Tubers

Zhang

Kouzuma

Miyaji

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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An Apios americana trypsin inhibitor, AATI, was purified from Apios tubers by chromatography on DEAE Cellulofine A-500 and Sephadex G-50. The molecular mass of AATI was determined to be 6,437 Da by matrix-assisted laser desorption and ionization time-of-flight mass spectrometer (MALDI-TOF-MS). It showed strong inhibitory activity toward serine proteases, and the inhibition constants toward trypsin and chymotrypsin were 3.0 x 10(-9) M and 1.0 x 10(-6) M respectively. The inhibitory activity was not affected by heating at 80 degrees C for 2 h or by incubation at a wide range of pH values, suggesting that AATI has remarkable heat-stability and pH-stability. AATI cDNA consists of 552 nucleotides, and includes an open reading frame encoding a protein of 116 amino acids. The results of N-terminal amino acid sequencing of AATI and MALDI-TOF-MS analysis suggested that the deduced amino acid sequence had 50 and seven extra amino acids at the N- and C-termini respectively. Thus the mature AATI protein consists of 59 amino acid residues. Comparison of the amino acid sequence with those of the trypsin inhibitors from plants suggests that AATI belongs to the Bowman-Birk family and that it contains two possible reactive sites toward trypsin at Lys62 and Arg88.

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Section: Cloning Of Aati Cdna and Sequence Analysismentioning

confidence: 99%

Purification, Characterization, and cDNA Cloning of a Bowman-Birk Type Trypsin Inhibitor fromApios americanaMedikus Tubers

Zhang

Kouzuma

Miyaji

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…The inhibitor was isolated and sequenced (Ceciliani et al, 1997) and its threedimensional structure in solution determined (Catalano et al, 2003). At the same time it was shown that it possesses anticarcinogenic activity against human breast and cervical cancer cells (Lanza et al, 2004) and the cDNA coding for it was isolated and its functional expression in response to biotic and abiotic stress studied (Balestrazzi et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Crystal structure of the anticarcinogenic Bowman–Birk inhibitor from snail medic (Medicago scutellata) seeds complexed with bovine trypsin

Capaldi

Perduca

Faggion

et al. 2007

Journal of Structural Biology

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“…In addition, congeneric competition resulted in down‐regulation of a Kunitz‐type trypsin inhibitor, a family involved in herbivore defence in the relative Medicago scutellata (Balestrazzi et al . ) and in poplar (Major & Constabel ), and edgeR detected three additional trypsin inhibitors responsive to competition. Interestingly, indirect interactions through shared enemies such as herbivores can play important roles in maintaining community diversity (Chesson & Kuang ), and the intensity of native herbivore damage is known to differ among an 18‐species panel of co‐occurring Trifolium (15 species) and Medicago (three species) at Bodega Bay (Parker & Gilbert ).…”

Section: Discussionmentioning

confidence: 94%

Transcriptomic responses to conspecific and congeneric competition in co‐occurring Trifolium

et al. 2017

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1. Species coexistence requires differential response to inter-and intraspecific competition, typically conceptualized as niche differentiation. Coexistence of close relatives therefore poses an interesting scenario with regards to niche differentiation since these species generally have many traits in common due to shared ancestry. Native Californian Trifolium assemblages are locally diverse and represent a unique system for understanding competitive interactions among close relatives.2. We conducted two similar greenhouse studies in which Trifolium fucatum was grown alone, with a conspecific competitor, and with a congeneric competitor (Trifolium macraei). In the first study, we assessed biomass production in T. fucatum, and in the second study we conducted an RNAseq analysis of T. fucatum roots to test for differentially expressed genes that may mediate competitive interactions and potentially coexistence.3. Compared to plants grown alone, competition (i.e. growth in the same pot) with a conspecific resulted in a greater reduction in biomass than competition with a congener, as predicted by theory. However, competition with a congener resulted in twice as many differentially expressed genes as competition with a conspecific. 4.Annotations of identified genes differentiating congeneric from conspecific competition suggest several functions attributed to interactions with third-party organisms, including nodulation with rhizobial mutualists, and defence responses against pathogens and herbivores. In addition, salt-responsive genes and an iron transporter were differentially expressed in congeneric competition, and comparisons of sodium and iron concentrations in field soils where these species are found showed that T. fucatum occurs in higher sodium and iron microsites than T. macraei. Thus, the transcriptome highlighted abiotic niche axes worth pursuing in future ecological experiments as potential mediators of coexistence. 5.We also found a subset of genes that responded similarly to both congeneric and conspecific competition in both direction and magnitude, indicating some conserved responses to competition, regardless of neighbour identity. 6. Synthesis. Transcriptomic analyses represent novel tools for identifying the molecular mechanisms underlying interactions among species. Working iteratively with ecological experimentation and observation, transcriptomes may allow us to identify novel dimensions of the n-dimensional niche that determine species' distributions and their ability to coexist.

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A trypsin inhibitor cDNA from a novel source, snail medic (Medicago scutellata L.): cloning and functional expression in response to wounding, herbivore, jasmonic and salicylic acid

Cited by 10 publications

References 45 publications

Purification, Characterization, and cDNA Cloning of a Bowman-Birk Type Trypsin Inhibitor fromApios americanaMedikus Tubers

Purification, Characterization, and cDNA Cloning of a Bowman-Birk Type Trypsin Inhibitor fromApios americanaMedikus Tubers

Crystal structure of the anticarcinogenic Bowman–Birk inhibitor from snail medic (Medicago scutellata) seeds complexed with bovine trypsin

Transcriptomic responses to conspecific and congeneric competition in co‐occurring Trifolium

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