A trypsin‐like protease in rice green semi‐looper, <i>Naranga aenescens</i> moore (lepidoptera: Noctuidae): purification and characterization

Zibaee, Arash; Bandani, Ali Reza; Fazeli‐Dinan, Mahmoud; Zibaee, Idin; Sendi, Jalal Jalali; Maleki, Feizollah A.

doi:10.1002/arch.20435

Cited by 18 publications

(20 citation statements)

References 25 publications

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“…(2008) found pH 8.0–10.5 as optimal pH for proteolytic activity in the larval midgut of Achaea janata L. (Lepidoptera: Noctuidae). Zibaee et al . (2011) demonstrated pH 10.0 for trypsin‐like and pH 9.0–10.0 for chymotrypsin‐like proteinases of Naranga aenescens Moore (Lepidoptera: Noctuidae).…”

Section: Resultsmentioning

confidence: 99%

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Proteolytic profile in the larval midgut of Chilo suppressalis Walker (Lepidoptera: Crambidae)

Zibaee

2012

Entomological Research

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Chilo suppressalis is a key constraint on production of rice. The current research was conducted to study the types of digestive proteases in the larval midgut of C. suppressalis. It was found that activity of total digestive proteases increased from the first to the fifth larval instars, which showed different nutritional requirements. Four types of proteinases and two types of exopeptidase were identified so that their activities from the highest to the lowest activities is trypsin‐like, chymotrypsin‐like and elastase for proteinases, and amino and carboxypeptidases for exopeptidases. Meanwhile, just one type of cysteine protease, cathepsin D, was determined in the fourth and fifth instar larvae. The optimal pH for activity of total protease was found to be pH 9–10 and optimal temperature was observed to be 35–40°C, where there was the highest proteolytic activity. Some specific inhibitors of proteases including PMSF, TLCK, TPCK, DTT, E‐64, cystatin, phenanthroline and EDTA were used to confirm the types of proteases in the midgut of C. suppressalis.

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Section: Resultsmentioning

confidence: 99%

“…Homogenates were separately transferred to 1.5‐mL centrifuge tubes and centrifuged at 20 000 g for 20 min at 4°C. The supernatants were pooled and stored at −20°C for subsequent analyses (Zibaee et al . 2011).…”

Section: Methodsmentioning

confidence: 99%

Proteolytic profile in the larval midgut of Chilo suppressalis Walker (Lepidoptera: Crambidae)

Zibaee

2012

Entomological Research

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“…For example, Mg 2ϩ increases trypsin-like protease activity in the midgut of the rice green semilooper, Naranga aenescens Moore (Zibaee et al 2011). Additionally, the population size of the root weevil, Diaprepes abbreviatus (L), is positively related to the Mg concentration in the soil (Li et al 2007).…”

Section: Discussionmentioning

confidence: 96%

Effects of Nitrogen Fertilizer and Magnesium Manipulation on the <I>Cnaphalocrocis medinalis</I> (Lepidoptera: Pyralidae)

Ge¹,

Wan²,

Jie³

et al. 2013

jnl. econ. entom.

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The rice leaffolder, Cnaphalocrocis medinalis Guenee (Lepidoptera: Pyralidae), is a rice pest that is prone to outbreaks when high levels of nitrogen (N) fertilizer are applied to rice. The larvae feed by scraping the mesophyll cells of leaves, which are mainly composed of chloroplasts containing significant levels of elemental magnesium (Mg). To determine the cause of N fertilizer-induced rice leaffolder outbreaks and the effects of Mg and Mg uptake on the rice leaffolder, the changes in the life history traits and biochemistry of C. medinalis larvae feeding on rice leaves treated with different Mg and N concentrations were investigated in this study. The results showed that foliar treatment using different Mg concentrations under hydroponic culture conditions significantly decreased the developmental duration (days) (DD) of the larvae and pupae but did not influence the pupation rate or adult emergence rate. The number of eggs laid by adult females and the protein content and total sugar content in the larvae and pupae significantly increased with increasing Mg concentrations. Under soil culture conditions, the DD for the larvae and number of eggs laid by adult females significantly increased with increasing nitrogen fertilizer levels, but the applied fertilizer did not influence the DD in the pupae, pupation rate, or adult emergence rate. In contrast, the DD for adults decreased with increased fertilizer levels. In hydroponic culture, Mg in the culture solution and Mg foliar sprays significantly increased the Mg content in rice leaves. In soil cultures, the use of N fertilizer and Mg foliar spray did not significantly increase the leaf Mg content, except at a concentration of 12.5 g/L, indicating that the leaf Mg content may be related to the root uptake of Mg.

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“…For example, the salivary gland proteinases involved in extraoral digestion in Heteroptera exhibit a low temperature optimum (Oliveira et al., ; Zibaee et al., ). Similarly, among‐ and within‐species differences in the efficiency of substrate cleavage and sensitivity to TLCK (Novillo et al., ; Lam et al., ; Oliveira et al., ; Zibaee et al., ) might reflect adaptation to a specific food. For instance, trypsins in some hematophagous insects do not hydrolize B‐R‐pNA and require longer peptide chains (Dametto et al., ; Lu et al., ).…”

Section: Structural Diversity Of Insect Digestive Trypsinsmentioning

confidence: 99%

Dietary and phylogenetic correlates of digestive trypsin activity in insect pests

Lazarević

Janković-Tomanić

2015

Entomologia Exp Applicata

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Because food proteins are crucial for insect survival, growth, and fecundity, enzymes involved in their digestion have attracted the attention of fundamental entomologists studying the mechanisms and patterns of dietary specialization, and applied entomologists searching for more efficient modes of pest control. Most insects digest proteins using trypsin, an endopeptidase that cleaves polypeptide chains on the carboxyl side of arginine and lysine, two basic amino acids. As the most ancestral proteinase, trypsin is wide-spread in the digestive tract of insects from various orders and with various feeding habits. The present review focuses on biochemical and molecular characteristics, mechanisms of regulation, and adaptive/non-adaptive changes of trypsin activity in response to heterogeneous food environments in a phylogenetic context. Within-and among-species variations in trypsin structure and regulation that contribute to better matching with specific food types are emphasized. We also discuss the relevance of these data for choosing an appropriate strategy for control of insect pests. Pest control strategies to reduce trypsin activity by interfering with substrate binding or trypsin synthesis and secretion have been suggested. Successful application of these procedures requires a multidisciplinary approach and knowledge about digestive physiology of the pest, as well as the structural characteristics of trypsins that determine their interaction with proteinaceous inhibitors and other food compounds, about patterns of developmental changes in trypsin gene expression, adaptive responses of insects to food composition, and various environmental effects on trypsin activity.

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A trypsin‐like protease in rice green semi‐looper, Naranga aenescens moore (lepidoptera: Noctuidae): purification and characterization

Cited by 18 publications

References 25 publications

Proteolytic profile in the larval midgut of Chilo suppressalis Walker (Lepidoptera: Crambidae)

Proteolytic profile in the larval midgut of Chilo suppressalis Walker (Lepidoptera: Crambidae)

Effects of Nitrogen Fertilizer and Magnesium Manipulation on the <I>Cnaphalocrocis medinalis</I> (Lepidoptera: Pyralidae)

Dietary and phylogenetic correlates of digestive trypsin activity in insect pests

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