A tumor microenvironment responsive nanoplatform with oxidative stress amplification for effective MRI-based visual tumor ferroptosis

Luo, Sanzhong; Ma, Di; Wei, Rui-Li; Wang, Yao; Pang, Xinrui; Wang, Ye; Xu, Xiangdong; Wei, Xinhua; Guo, Yuan; Jiang, Xinqing; Yuan, Youyong; Yang, Ruimeng

doi:10.1016/j.actbio.2021.11.007

Cited by 44 publications

(32 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relaxation time of wild-type 4T1 cells after their incubation with FAS was not measured. It is known that, in cells dying by ferroptosis, the intensity of the MR signal may increase [ 14 , 15 ], and this experiment was deemed inappropriate in our case (cf. FAS toxicity data presented above).…”

Section: Resultsmentioning

confidence: 99%

New Approach to Non-Invasive Tumor Model Monitoring via Self-Assemble Iron Containing Protein Nanocompartments

et al. 2022

View full text Add to dashboard Cite

According to the World Health Organization, breast cancer is the most common oncological disease worldwide. There are multiple animal models for different types of breast carcinoma, allowing the research of tumor growth, metastasis, and angiogenesis. When studying these processes, it is crucial to visualize cancer cells for a prolonged time via a non-invasive method, for example, magnetic resonance imaging (MRI). In this study, we establish a new genetically encoded material based on Quasibacillus thermotolerans (Q.thermotolerans, Qt) encapsulin, stably expressed in mouse 4T1 breast carcinoma cells. The label consists of a protein shell containing an enzyme called ferroxidase. When adding Fe2+, a ferroxidase oxidizes Fe2+ to Fe3+, followed by iron oxide nanoparticles formation. Additionally, genes encoding mZip14 metal transporter, enhancing the iron transport, were inserted into the cells via lentiviral transduction. The expression of transgenic sequences does not affect cell viability, and the presence of magnetic nanoparticles formed inside encapsulins results in an increase in T2 relaxivity.

show abstract

Section: Resultsmentioning

confidence: 99%

New Approach to Non-Invasive Tumor Model Monitoring via Self-Assemble Iron Containing Protein Nanocompartments

et al. 2022

View full text Add to dashboard Cite

show abstract

“…A combination of amphiphilic block copolymers with SNCs could efficiently enhance their stability, biocompatibility, and environmental sensitivity. 20 , 21 Nevertheless, the drug release rate could also be accelerated by functionalizing SNCs with block copolymers. Therefore, to obtain controlled drug delivery systems, it is critical to functionalize SNCs with amphiphilic block copolymers with regularly tailored chemical structures and physicochemical properties.…”

Section: Introductionmentioning

confidence: 99%

“…Amphiphilic block copolymers are polymer chains with hydrophilic and hydrophobic blocks, which exhibit the advantage of good controllability, biocompatibility, and structural versatility. − They were able to possess convertible chain conformation, controlled interfacial properties, and a stimuli-responsive chemical structure. A combination of amphiphilic block copolymers with SNCs could efficiently enhance their stability, biocompatibility, and environmental sensitivity. , Nevertheless, the drug release rate could also be accelerated by functionalizing SNCs with block copolymers. Therefore, to obtain controlled drug delivery systems, it is critical to functionalize SNCs with amphiphilic block copolymers with regularly tailored chemical structures and physicochemical properties. , The LBL deposition allows for the buildup of the functional nanocomposites with environmental responsive properties for the controlled release of functional reagents from the defined-structure matrices. , However, few studies were conducted on the buildup of LBL films based on responsive block copolymer-functionalized SNCs for drug delivery.…”

Section: Introductionmentioning

confidence: 99%

Steric Effects in the Deposition Mode and Drug-Delivering Efficiency of Nanocapsule-Based Multilayer Films

Chu

Zhang

et al. 2022

ACS Omega

View full text Add to dashboard Cite

Using surface-initiated atom transfer radical polymerization (ATRP), block polymers with a series of quaternization degrees were coated on the surface of silica nanocapsules (SNCs) by the “grafting-from” technique. Molnupiravir, an antiviral medicine urgently approved for the treatment of SARS-CoV-2, was encapsulated in polymer-coated SNCs and further incorporated into well-defined films with polystyrene sulfonate (PSS) homopolymers by layer-by-layer (LBL) self-assembly via electrostatic interactions. We investigated the impact of the quaternization degree of the polymers and steric hindrance of functional groups on the growth mode, swelling/deswelling transition, and drug-delivering efficiency of the obtained LBL films. The SNCs were derived from coronas of parent block polymers of matched molecular weights—poly( N -isopropylacrylamide)- block -poly( N , N -dimethylaminoethyl methacrylate) (PNIPAM- b -PDMAEMA)—by quaternization with methyl sulfate. As revealed by the data results, SNCs with coronas with higher quaternization degrees resulted in a larger layering distance of the film structure because of weaker ionic pairing (due to the presence of a bulky methyl spacer) between SNCs and PSS. Interestingly, when comparing the drug release profile of the encapsulated drugs from SNC-based films, the release rate was slower in the case of capsule coronas with higher quaternization degrees because of the larger diffusion distance of the encapsulated drugs and stronger hydrophobic–hydrophobic interactions between SNCs and drug molecules.

show abstract

“…Magnetic resonance imaging (MRI) is characterized by high resolution, no ionizing radiation, and unlimited signal penetration depth [3a, 10] . Especially, high‐field MRI (≥7T) enables the improvement of the signal‐to‐noise ratio and the exertion of higher spatial resolution to visualize microscopic objects (100 μm in size), compared with low‐field MRI (1.5T or 3T) currently used in clinics (1 mm in size) [3b, 5c, 11] . Nevertheless, there is still a lack of proper high‐field MRI contrast agents for monitoring ferroptosis, because the conventional agents generally induced strong negative contrast so as to limit the imaging accuracy in high‐field MRI [11b, 12] .…”

Section: Introductionmentioning

confidence: 99%

Ternary Alloy PtWMn as a Mn Nanoreservoir for High‐Field MRI Monitoring and Highly Selective Ferroptosis Therapy

et al. 2022

View full text Add to dashboard Cite

Ferroptosis exhibits potential to damage drugresistant cancer cells. However, it is still restricted with the "off-target" toxicity from the undesirable leakage of metal ions from ferroptosis agents, and the lack of reliable imaging for monitoring the ferroptosis process in living systems. Herein, we develop a novel ternary alloy PtWMn nanocube as a Mn reservoir, and further design a microenvironment-triggered nanoplatform that can accurately release Mn ions within the tumor to increase reactive oxygen species (ROS) generation, produce O 2 and consume excess glutathione for synergistically enhancing nonferrous ferroptosis. Moreover, this nanoplatform exerts a responsive signal in high-field magnetic resonance imaging (MRI), which enables the real-time report of Mn release and the monitoring of ferroptosis initiation through the signal changes of T 1 -/ T 2 -MRI. Thus, our nanoplatform provides a novel strategy to store, deliver and precisely release Mn ions for MRI-guided high-specificity ferroptosis therapy.

show abstract

A tumor microenvironment responsive nanoplatform with oxidative stress amplification for effective MRI-based visual tumor ferroptosis

Cited by 44 publications

References 29 publications

New Approach to Non-Invasive Tumor Model Monitoring via Self-Assemble Iron Containing Protein Nanocompartments

New Approach to Non-Invasive Tumor Model Monitoring via Self-Assemble Iron Containing Protein Nanocompartments

Steric Effects in the Deposition Mode and Drug-Delivering Efficiency of Nanocapsule-Based Multilayer Films

Ternary Alloy PtWMn as a Mn Nanoreservoir for High‐Field MRI Monitoring and Highly Selective Ferroptosis Therapy

Contact Info

Product

Resources

About