A Tumor-Specific Ferric-Coordinated Epigallocatechin-3-gallate cascade nanoreactor for glioblastoma therapy

Mu, Mingchun; Chen, Haifeng; Fan, Rangrang; Wang, Yuelong; Tang, Xin; Lan, Mei; Zhao, Na; Zou, Bingwen; Tong, Aiping; Xu, Jianguo; Han, Bo; Guo, Gang

doi:10.1016/j.jare.2021.07.010

Cited by 29 publications

(10 citation statements)

References 52 publications

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“…Our results are consistent with previous studies that show that uptake of EGCG-conjugated nanocomposites is significantly higher than that without EGCG as a ligand [ 13 , 34 , 35 ]. In the current study, 67LR appears to mediate the enhancement effects of EGCG, which do not require immobilization of EGCG on the surface of nanoparticles.…”

Section: Discussionsupporting

confidence: 93%

“…Such effects may facilitate intracellular delivery of nanocarriers with therapeutic agents. Nanoparticles with immobilized EGCG have been shown to exhibit enhanced uptake by tumor cells [ 34 , 35 ] and enhanced therapeutic efficacy in tumor models [ 13 , 34 , 35 , 36 , 37 ]. Limited evidence indicates that the 67LR antibody attenuates the enhancement effects of EGCG on cellular uptake of EGCG nanocomposites [ 34 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

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Laminin Receptor-Mediated Nanoparticle Uptake by Tumor Cells: Interplay of Epigallocatechin Gallate and Magnetic Force at Nano–Bio Interface

Hsu

et al. 2022

Pharmaceutics

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Epigallocatechin gallate (EGCG), a major tea catechin, enhances cellular uptake of magnetic nanoparticles (MNPs), but the mechanism remains unclear. Since EGCG may interact with the 67-kDa laminin receptor (67LR) and epidermal growth factor receptor (EGFR), we investigate whether a receptor and its downstream signaling may mediate EGCG’s enhancement effects on nanoparticle uptake. As measured using a colorimetric iron assay, EGCG induced a concentration-dependent enhancement effect of MNP internalization by LN-229 glioma cells, which was synergistically enhanced by the application of a magnetic field. Transmission electron microscopy demonstrated that EGCG increased the number, but not the size, of internalized vesicles, whereas EGCG and the magnet synergistically increased the size of vesicles. EGCG appears to enhance particle–particle interaction and thus aggregation following a 5-min magnet application. An antibody against 67LR, knockdown of 67LR, and a 67LR peptide (amino acid 161–170 of 67LR) attenuated EGCG-induced MNP uptake by 35%, 100%, and 45%, respectively, suggesting a crucial role of 67LR in the effects of EGCG. Heparin, the 67LR-binding glycosaminoglycan, attenuated EGCG-induced MNP uptake in the absence, but not presence, of the magnet. Such enhancement effects of EGCG were attenuated by LY294002 (a phosphoinositide 3-kinase inhibitor) and Akt inhibitor, but not by agents affecting cGMP levels, suggesting potential involvement of signaling downstream of 67LR. In contrast, the antibody against EGFR exerted no effect on EGCG-enhanced internalization. These results suggest that 67LR may be potentially amenable to tumor-targeted therapeutics.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Laminin Receptor-Mediated Nanoparticle Uptake by Tumor Cells: Interplay of Epigallocatechin Gallate and Magnetic Force at Nano–Bio Interface

Hsu

et al. 2022

Pharmaceutics

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“…In addition, amplified oxidative stress is induced by reducible disulfide bonds, ultimately achieving potentiated CDT/CT efficacy on glioblastoma. 238 The cationic polymer coating can significantly promote the adhesion of NPs in the tumor vascular system and the internalization of tumor endothelial cells. However, if the surface has a high positive charge, it will interact and aggregate with blood components, causing a human immune response, which is accessible to be removed and is toxic.…”

Section: Strategymentioning

confidence: 99%

“…They continuously released EGCG and Fe 3+ can slowly produce Fe 2+ , conduct a sufficient Fenton reaction to produce ROS, and enhance the chemotherapy effect of DOX. In addition, amplified oxidative stress is induced by reducible disulfide bonds, ultimately achieving potentiated CDT/CT efficacy on glioblastoma …”

Section: Combination Membranes Coating Strategymentioning

confidence: 99%

Surface Membrane Coating as a Versatile Platform for Modifying Antitumor Nanoparticles

Wang

et al. 2022

ACS Materials Lett.

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Antitumor nanoparticles now confront several obstacles, including poor biocompatibility and biodegradability, quick removal, and a short cycle time, given the growing interest in surface membrane coating as a versatile platform for altering nanoparticles and overcoming the limitations of current nanodrug delivery methods. This paper reviews the recent progress of cells, synthetic polymers, polyphenols, and polysaccharides membrane coatings with favorable biocompatibility and biodegradability and easy surface modification on the surface of antitumor nanoparticle platforms, which can provide a promising strategy for endowing nanoparticles with specific and expected performance to optimize the efficiency of antitumor. Furthermore, multiple membrane combination strategies and additional biorecognition ligands modification on the membrane surface are supplied in an attempt to tackle the challenges of single membrane coating in a variety of tumor therapeutic applications and ultimately facilitate effective clinical transformation.

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“…Theoretically, such a material could be implanted in the resection cavity of GBM and provide continuous, cell-free biocatalytic functions such as breakdown of immunosuppressive circulating molecules or generation of immunostimulatory agents through designed biocatalytic cascades. Injectable immunotherapies have previously been designed with multiphase functionality to track to and enter tumor cells and dissociate active molecules responsive to intracellular environments [66], but an implantable material with constant biocatalytic potential eliminates the need for dosing and relevant systemic toxicity. Cell-free stabilized enzymes have long been used in other industries with activity lifetimes approaching one year in industrial reactor environments for biomanufacturing [67].…”

Section: Implantable Nanoreactorsmentioning

confidence: 99%

Applying Synthetic Biology with Rational Design to Nature’s Greatest Challenges: Bioengineering Immunotherapeutics for the Treatment of Glioblastoma

Mashouf

Shah

et al. 2021

Immuno

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Improvements in bioengineering methodology and tools have allowed for significant progress in the development of therapeutics and diagnostics in medicine, as well as progress in many other diverse industries, such as materials manufacturing, food and agriculture, and consumer goods. Glioblastomas present significant challenges to adequate treatment, in part due to their immune-evasive and manipulative nature. Rational-design bioengineering using novel scaffolds, biomaterials, and inspiration across disciplines can push the boundaries in treatment development to create effective therapeutics for glioblastomas. In this review, we will discuss bioengineering strategies currently applied across diseases and disciplines to inspire creative development for GBM immunotherapies.

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A Tumor-Specific Ferric-Coordinated Epigallocatechin-3-gallate cascade nanoreactor for glioblastoma therapy

Abstract: Graphical abstract

Cited by 29 publications

References 52 publications

Laminin Receptor-Mediated Nanoparticle Uptake by Tumor Cells: Interplay of Epigallocatechin Gallate and Magnetic Force at Nano–Bio Interface

Laminin Receptor-Mediated Nanoparticle Uptake by Tumor Cells: Interplay of Epigallocatechin Gallate and Magnetic Force at Nano–Bio Interface

Surface Membrane Coating as a Versatile Platform for Modifying Antitumor Nanoparticles

Applying Synthetic Biology with Rational Design to Nature’s Greatest Challenges: Bioengineering Immunotherapeutics for the Treatment of Glioblastoma

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