A tunable nanopore sensor for the detection of metal ions using translocation velocity and biphasic pulses

Mayne, Laura J.; Christie, S.; Platt, Mark

doi:10.1039/c6nr07224k

Cited by 41 publications

(43 citation statements)

References 59 publications

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“…In the absence of convection, the velocity of the carrier can be proportional to the surface charge or zeta potential (Blundell et al, ; Kozak et al, ). Here, the velocity is reported as 1/ T 0.5 values (Mayne et al, ), which we have previously shown capable of monitoring the length and packing density of DNA around SPBs (Blundell et al, ). As the density of the DNA and/ or its length increases, the velocity of the SPB through the pore also increases.…”

Section: Resultsmentioning

confidence: 99%

“…For example, they have been applied to the detection of metabolites (Alsager et al, ), proteins (Billinge, Broom, & Platt, ; Billinge & Platt, ; Siwy et al, ), DNA sequencing (Branton et al, ; Majd et al, ; Yeh, Zhang, Qian, & Hsu, ), epigenetics (Healey, Rowe, Siati, Sivakumaran, & Platt, ; Rand et al, ) and cellular vesicles (Kamińska et al, ; Nizamudeen, Markus, Lodge, Parmenter, & Platt, ). An increasingly common strategy in RPS utilizes nanomaterials as carriers of recognition elements to selectively bind to the target (Ali, Nasir, & Ensinger, ; Billinge et al, ; Billinge & Platt, , ; Lin, Ivanov, & Edel, ; Mayne, Christie, & Platt, ; Rotem, Jayasinghe, Salichou, & Bayley, ; Sze, Ivanov, Cass, & Edel, ). The carrier acts as a support for multiple ligands enhancing the signal and facilitating multiplexed assays (Blundell, Mayne, Billinge, & Platt, ; Platt, Willmott, & Lee, ).…”

Section: Introductionmentioning

confidence: 99%

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Incorporating peptide aptamers into resistive pulse sensing

Maugi

Salkenova²,

Platt

2020

Med Devices & Sens

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The use of nanocarriers within resistive pulse sensing, RPS, aids the detection and quantification of analytes. In the absence of convection, the signal strength and frequency can dependent upon the electrophoretic mobility of the nanocarrier/analyte. Here, we have developed a simple strategy to incorporate peptide aptamers onto RPS assays with enhanced electrophoretic signals. Using a hybrid DNA–Peptide nanocarrier, an existing peptide was incorporated into a rapid assay without having to engineer or modify the peptide sequence. The surface of a nanocarrier is coated with a mixture of peptide aptamers and a non‐binding DNA. The binding of the target to the peptide creates an “analyte corona” which shields the phosphate groups of the underlying DNA. This results in a change in electrophoretic mobility of the nanocarrier. The signal is concentration‐dependent and is illustrated using a peptide to a key biomarker of infection, C‐reactive protein, CRP. As a comparison, we also show the binding of the CRP to a DNA aptamer. This universal approach can be easily adapted to other peptides without the peptide itself to undergo any chemical modifications opening new opportunities and applications in RPS strategies.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Incorporating peptide aptamers into resistive pulse sensing

Maugi

Salkenova²,

Platt

2020

Med Devices & Sens

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“…Second, measuring the translocation rates and velocities of the aptamer sequences through the pores, or finally the use of nanomaterials as carriers for multiple aptamers. 15,[21][22][23][24] The latter is appealing when the nanomaterials are Superparamagnetic Beads, SPB's, as the particles can perform both the purification and sensing roles. By adopting a tertiary structure in the presence of the target, the charge density around the SPB is altered.…”

Section: Introductionmentioning

confidence: 99%

“…[29][30][31][32] In cases where the charge double layers around the particles and pore walls interact, conductive pulses and ion current rectification can also be observed. 21,33,34 To help understand how the charge density effects the sensitivity of Aptamer RPS assays here we choose two known aptamers to heavy metal ions. The field of DNA-Metal ion sensors is growing and comprehensive review of the topic can be found elsewhere.…”

Section: Introductionmentioning

confidence: 99%

The Design and Characterization of Multifunctional Aptamer Nanopore Sensors

et al. 2018

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Aptamer-modified nanomaterials provide a simple, yet powerful sensing platform when combined with resistive pulse sensing technologies. Aptamers adopt a more stable tertiary structure in the presence of a target analyte, which results in a change in charge density and velocity of the carrier particle. In practice the tertiary structure is specific for each aptamer and target, and the strength of the signal varies with different applications and experimental conditions. Resistive pulse sensors (RPS) have single particle resolution, allowing for the detailed characterization of the sample. Measuring the velocity of aptamer-modified nanomaterials as they traverse the RPS provides information on their charge state and densities. To help understand how the aptamer structure and charge density effects the sensitivity of aptamer-RPS assays, here we study two metal binding aptamers. This creates a sensor for mercury and lead ions that is capable of being run in a range of electrolyte concentrations, equivalent to river to seawater conditions. The observed results are in excellent agreement with our proposed model. Building on this we combine two aptamers together in an attempt to form a dual sensing strand of DNA for the simultaneous detection of two metal ions. We show experimental and theoretical responses for the aptamer which creates layers of differing charge densities around the nanomaterial. The density and diameter of these zones effects both the viability and sensitivity of the assay. While this approach allows the interrogation of the DNA structure, the data also highlight the limitations and considerations for future assays.

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“…In this sense, great effort has been made by the scientific community in order to develop devices and systems for metal ions detection. Electrochemical devices were initially proposed and in the last few years these kind of devices achieved high sensitivity and, in some special cases, also high selectivity by using nanomaterials as active electrodes [2][3][4]. However, these types of devices normally suffer the interferences from the electromagnetic noise sources.…”

Section: Introductionmentioning

confidence: 99%

Portable Multispectral Colorimeter for Metallic Ions Detection and Classification

Braga¹,

Jaimes²,

Borysow³

et al. 2017

Preprint

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In the present work, a portable device system applied in the detection and classification of different metallic ions was proposed and developed, aiming at its application in the monitoring of hydrological systems like rivers, lakes and groundwater. Considering the system features, a portable colorimetric system was developed by using a multispectral optoelectronic sensor. All the technology of quantification and classification of metallic ions using Optoelectronic Multispectral sensors was fully integrated in the embedded hardware FPGA technology and software based on virtual Instrumentation (NI LabView ®). The system was used as a colorimeter by using the chromogen reagent of 1-(2-pyridylazo)-2-naphthol (PAN) as an indicator and the results obtained together with the signal processing and pattern analysis using the method of the linear discriminant analysis, allowed to obtain excellent results in detection and classification of Pb(II), Cd(II), Zn(II), Cu(II), Fe(III) and Ni(II) ions, with almost the same level of performance in relation to those obtained from the UV-VIS spectrometers of high spectral resolution.

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A tunable nanopore sensor for the detection of metal ions using translocation velocity and biphasic pulses

Cited by 41 publications

References 59 publications

Incorporating peptide aptamers into resistive pulse sensing

Incorporating peptide aptamers into resistive pulse sensing

The Design and Characterization of Multifunctional Aptamer Nanopore Sensors

Portable Multispectral Colorimeter for Metallic Ions Detection and Classification

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