A tunable pH probe scaffold based on sulfonamide rhodamine and its application in mitochondrial pH research

Qiu, Jianwen; Zhong, Chunli; Li, Meng; Xiong, Xinyi; Gao, Yong; Zhu, Hu

doi:10.1016/j.snb.2022.132606

Cited by 7 publications

(3 citation statements)

References 42 publications

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“…10). 162 Owing to the electron-withdrawing ability of the sulfonamide group, the probe was a non-fluorescent spirolactone under acidic conditions. With an increase in the pH, the sulfonamide protons dissociate and form sulfonamide anions with electron-donating ability, which resulted in the ring opening of rhodamine spirolactone, thus restoring the strong fluorescence emission of rhodamine.…”

Section: Rhodamine Derivatives For Bioimagingmentioning

confidence: 99%

Fluorescent dyes based on rhodamine derivatives for bioimaging and therapeutics: recent progress, challenges, and prospects

et al. 2023

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Section: Rhodamine Derivatives For Bioimagingmentioning

confidence: 99%

Fluorescent dyes based on rhodamine derivatives for bioimaging and therapeutics: recent progress, challenges, and prospects

et al. 2023

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“…The detection techniques primarily rely on principles such as fluorescence and Surface-enhanced Raman Scattering (SERS) [ 15 , 16 ] . Although fluorescence imaging is widely utilized [ 17 , 18 ], it suffers from issues like easy quenching of fluorescent dyes and the arduous removal of cell autofluorescence [ 19 , 20 ]. These problems result in non-repeatable measurements and background interference.…”

Section: Introductionmentioning

confidence: 99%

SERS-based long-term mitochondrial pH monitoring during differentiation of human induced pluripotent stem cells to neural progenitor cells

Yang,

Bu,

et al. 2024

Biomed. Opt. Express

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As one of the important organelles in the process of cell differentiation, mitochondria regulate the whole process of differentiation by participating in energy supply and information transmission. Mitochondrial pH value is a key indicator of mitochondrial function. Therefore, real-time monitoring of mitochondrial pH value during cell differentiation is of great significance for understanding cell biochemical processes and exploring differentiation mechanisms. In this study, Surface-enhanced Raman scattering (SERS) technology was used to achieve the real-time monitoring of mitochondrial pH during induced pluripotent stem cells (iPSCs) differentiation into neural progenitor cells (NPCs). The results showed that the variation trend of mitochondrial pH in normal and abnormal differentiated batches was different. The mitochondrial pH value of normal differentiated cells continued to decline from iPSCs to embryoid bodies (EB) day 4, and continued to rise from EB day 4 to the NPCs stage, and the mitochondrial microenvironment of iPSCs to NPCs differentiation became acidic. In contrast, the mitochondrial pH value of abnormally differentiated cells declined continuously during differentiation. This study improves the information on acid-base balance during cell differentiation and may provide a basis for further understanding of the changes and regulatory mechanisms of mitochondrial metabolism during cell differentiation. This also helps to improve more accurate and useful differentiation protocols based on the microenvironment within the mitochondria, improving the efficiency of cell differentiation.

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“…Normal mitochondrial pH is known to be alkaline, and nevertheless, mitochondrial dysregulation during cuproptosis leads to abnormal ion influx, which may affect the pH of the mitochondrial matrix . Meanwhile, it has been indicated that the pH value of mitochondria shows a significant part in their physiological functions. − The normal cellular mitochondrial pH range is slightly alkalescent (pH ≈ 8.00) . The pH of mitochondria has a direct impact on processes such as signal transmission, apoptosis, and the generation of ROS .…”

Section: Introductionmentioning

confidence: 99%

Polysiloxane-Based Molecular Logic Gate for Dual-Channel Visualizing Mitochondrial pH and Sulphite Changes during Cuproptosis

Wang,

Lan,

Zuo

2023

Anal. Chem.

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Intracellular Cu-induced regulated cell death, characterized by the aggregation of lipidizing mitochondrial enzymes, is called cuproptosis. Mitochondria play a vital role in the metabolic regulation of cell injury and stressful immune responses. The pH levels and sulfur dioxide (SO 2 ) content in mitochondria have important indicative roles in the regulation of cuproptosis. However, fluorescent probes that simultaneously detect changes in pH and SO 2 in mitochondria during cuprotosis have not been reported. To fill this blank, in this study, we dexterously used functional polysiloxane as a fluorescent platform to propose a molecular logic gate probe P 0 -pH-SO 2 for detecting changes in intramitochondrial pH and SO 2 content through a dualchannel mode. In addition, we defined a new function to reflect the cellular state of the elesclomol-induced cuproptosis process based on the input and output of the relevant logic relationship. This new fluorescent molecular logic gate probe P 0 -pH-SO 2 can be rapidly activated by mitochondrial sulfites to induce green fluorescence, while the red fluorescence is quenched with the proton in the mitochondria. Overall, this study developed a novel logic-gated molecular probe that provided a versatile strategy for monitoring the role played by intramitochondrial sulfites and H + in cuproptosis. This work will open the way to broaden the applications of molecular logic gates and fluorescent polysiloxanes.

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A tunable pH probe scaffold based on sulfonamide rhodamine and its application in mitochondrial pH research

Cited by 7 publications

References 42 publications

Fluorescent dyes based on rhodamine derivatives for bioimaging and therapeutics: recent progress, challenges, and prospects

Fluorescent dyes based on rhodamine derivatives for bioimaging and therapeutics: recent progress, challenges, and prospects

SERS-based long-term mitochondrial pH monitoring during differentiation of human induced pluripotent stem cells to neural progenitor cells

Polysiloxane-Based Molecular Logic Gate for Dual-Channel Visualizing Mitochondrial pH and Sulphite Changes during Cuproptosis

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