A “turn-on” fluorescent probe for the detection of Cu²⁺ in living cells based on a signaling mechanism of NN isomerization

Abstract

A “turn-on” probe A based on coumarin with a mechanism of NN isomerization was developed for detecting Cu2+ in living cells.

Azo‐Based Fluorogenic Probes for Biosensing and Bioimaging: Recent Advances and Upcoming Challenges

“…a) Structures of azoanilinedyes recently developedwith the dual aim of widening the working pH range and improving the sensitivity of this unusual class of copper(II)-sensitive probes (DL = detection limit). [92][93][94] b) Structures of bifunctional azoaniline-based chromo-/fluorogenicp robes for the dualdetection of fluoride and copper(II)i ons (left) or nervea gent mimic diethyl chlorophosphate (DCP)and copper(II) ions (right). [95] small-molecule fluorescent probes, the activation mechanism of which was based on chemical/enzymatic deactivation of the (di)aryl azo unit, were discussed.…”

“…This wasa chieved with coumarin-azo dye C,w hich exhibited excellent sensitivity within awiderange of pH (3-10). [92] An unprecedented detection limit of 20 nm at physiological pH was obtained (in addition, al ower value of 14.4 nm wasr eported very recently with azoaniline TPI-AD). [93] Conversely,t here has been no significant improvementi nt he sensitivity with azoaniline dye D (detection limit = 10 mm), despite ab road working pH range (5)(6)(7)(8)(9)(10)(11) for fluorescenceturn ON detection of copper(-II) analyte.…”

“…More recently, the same fluorescence turn ON signaling mechanism was implemented to more sophisticated anilines with the dual aim of widening the working pH range and improving the sensitivity of this unusual class of copper(II)‐sensitive probes (Figure a). This was achieved with coumarin–azo dye C , which exhibited excellent sensitivity within a wide range of pH (3–10) . An unprecedented detection limit of 20 n m at physiological pH was obtained (in addition, a lower value of 14.4 n m was reported very recently with azoaniline TPI‐AD) .…”

Novel edaravone-based azo dyes: efficient synthesis, characterization, antibacterial activity, DFT calculations and comprehensive investigation of the solvent effect on the absorption spectra

“…3 These important discoveries led to the development of azo dyes, which are now being considered as the most important group of the organic dyes. [4][5][6][7][8] These dyes contain one azo group (-N]N-) in their structures but can be in the form of diazo, triazo, tetraazo or more (polyazo). In addition to using these compounds as color, the azo dyes are implemented in many industrial processes such as printing, textile, photography, color additives, medicine and molecular diagnosis.…”

A Highly Selective Fluorescent Probe for Zn2+ Based on a Rhodamine-6G dye Derivative Modified by a Furan Unit

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In recent years, the design and exploration of selective and sensitive fluorescent probes for the detection of biological and environmental transition metal ions have received profound attention. [1][2][3][4][5][6] Among transition metal ions, Zn 2+ plays a critical role in biological, environmental and chemical systems. [7][8][9][10] Nevertheless, disruption of the Zn 2+ balance is dangerous to human health.

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