“…In practice, the actual constant value of I * does not necessarily equal 0.5, depending instead on the measured pixel intensity of a premixed solution. To incorporate this into the calculation of mixing index, the following equation has been proposed for HSI images: where HSI ,mix is the normalized premixed pixel intensity, HSI ,unmix is the normalized pixel intensity prior to mixing, and HSI ,i is where HSI ,i is the normalized pixel intensity inside the image, whereas I HSI, min and I gray, max are the minimum and maximum local pixel intensity, respectively, and I HSI, i is …”