A two-component gene (NTHK1) encoding a putative ethylene-receptor homolog is both developmentally and stress regulated in tobacco

Zhang, J.-S.; Xie, Can; Shen, Yuntian; Chen, S.-Y.

doi:10.1007/s001220000469

Cited by 47 publications

(78 citation statements)

References 38 publications

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“…Previously, we have cloned tobacco ethylene receptor genes NTHK1 and NTHK2, and studied their expression in response to different abiotic stresses. We found that both genes were induced by wounding and dehydration (Zhang et al, 1999a(Zhang et al, , 2001a(Zhang et al, , 2001b. In situ mRNA hybridization and immunohistochemistry analysis of the NTHK1 protein revealed that both mRNA and protein appeared first in the palisade cell layer upon cutting and then gradually spread to other sponge cells of the leaf (Zhang et al, 2001a;Xie et al, 2002).…”

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confidence: 88%

“…We found that both genes were induced by wounding and dehydration (Zhang et al, 1999a(Zhang et al, , 2001a(Zhang et al, , 2001b. In situ mRNA hybridization and immunohistochemistry analysis of the NTHK1 protein revealed that both mRNA and protein appeared first in the palisade cell layer upon cutting and then gradually spread to other sponge cells of the leaf (Zhang et al, 2001a;Xie et al, 2002). When exposed to salt stress, the NTHK1 gene expression was induced, whereas the NTHK2 expression was not significantly affected (Zhang et al, 2001a(Zhang et al, , 2001b.…”

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confidence: 89%

“…Because the NTHK1 mRNA accumulation was observed in response to salt stress in tobacco seedlings (Zhang et al, 2001a), this gene may participate in saltstress responses in plants. We tested the responses of the transgenic Arabidopsis under salt-stress conditions.…”

Section: Nthk1 Increases Salt Sensitivity Of the Transgenic Plants Anmentioning

confidence: 99%

“…In tomato, six receptor genes have been cloned, and their functions in fruit ripening and defense response have been investigated (Ciardi et al, , 2001Tieman et al, 2000;Klee, 2002Klee, , 2004. In tobacco, four receptor genes have been isolated, and two of them, NTHK1 (N. tabacum histidine kinase 1) and NTHK2, represent subfamily II members (Knoester et al, 1997;Zhang et al, 1999aZhang et al, , 2001aZhang et al, , 2001bTerajima et al, 2001). Both NTHK1 and NTHK2 have four hydrophobic regions, a GAF domain, a diverged His kinase domain, and a receiver domain.…”

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confidence: 99%

“…In situ mRNA hybridization and immunohistochemistry analysis of the NTHK1 protein revealed that both mRNA and protein appeared first in the palisade cell layer upon cutting and then gradually spread to other sponge cells of the leaf (Zhang et al, 2001a;Xie et al, 2002). When exposed to salt stress, the NTHK1 gene expression was induced, whereas the NTHK2 expression was not significantly affected (Zhang et al, 2001a(Zhang et al, , 2001b. This differential expression pattern suggests different roles of various receptors in multiple stress responses and also implies a possible specific function for NTHK1 in plant saltstress responses.…”

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confidence: 99%

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Modulation of Ethylene Responses Affects Plant Salt-Stress Responses

et al. 2006

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Ethylene signaling plays important roles in multiple aspects of plant growth and development. Its functions in abiotic stress responses remain largely unknown. Here, we report that alteration of ethylene signaling affected plant salt-stress responses. A type II ethylene receptor homolog gene NTHK1 (Nicotiana tabacum histidine kinase 1) from tobacco (N. tabacum) conferred salt sensitivity in NTHK1-transgenic Arabidopsis (Arabidopsis thaliana) plants as judged from the phenotypic change, the relative electrolyte leakage, and the relative root growth under salt stress. Ethylene precursor 1-aminocyclopropane-1-carboxylic acid suppressed the salt-sensitive phenotype. Analysis of Arabidopsis ethylene receptor gain-of-function mutants further suggests that receptor function may lead to salt-sensitive responses. Mutation of EIN2, a central component in ethylene signaling, also results in salt sensitivity, suggesting that EIN2-mediated signaling is beneficial for plant salt tolerance. Overexpression of the NTHK1 gene or the receptor gain-of-function activated expression of salt-responsive genes AtERF4 and Cor6.6. In addition, the transgene NTHK1 mRNA was accumulated under salt stress, suggesting a posttranscriptional regulatory mechanism. These findings imply that ethylene signaling may be required for plant salt tolerance.

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confidence: 88%