A two-jet plasmatron for the spectrochemical analysis of geological samples

Yudelevich, I.G.; Cherevko, A. S.; Engelsht, V. S.; Pikalov, V. V.; Tagil'tsev, A. P.; Zheenbajev, Zh.Zh.

doi:10.1016/0584-8547(84)80086-5

Cited by 32 publications

(26 citation statements)

References 4 publications

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“…All powders (5-7 mg per one analysis) were used for metal composition analysis by two-jet arc plasmatron atomic emission according to [28,29]. The mass percentage of metals (% of powder containing all dried components of eluate) was determined.…”

Section: Methodsmentioning

confidence: 99%

“…FPLC gel filtration of IgGs under the "acid shock" conditions was carried out using Superdex 200 HR 10/30 (Pharmacia) as described previously [20][21][22][23][24][25][26][27][28][29][30][31][32]. Fractions were collected, neutralized, dialyzed against 20 mM Tris-HCl (pH 7.5), and used for the activity assays.…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of proteolytic activity of IgGs after SDS-PAGE was performed as for other Abz activities [15,16,[20][21][22][23][24][25][26][27][28][29][30][31][32]. To restore the MBP-hydrolyzing activity of IgGs after SDS-PAGE, SDS was removed by incubating the gel for 1 h at 30 • C with 4 M urea and washing 10 times with H 2 O.…”

Section: Methodsmentioning

confidence: 99%

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Metal-dependent hydrolysis of myelin basic protein by IgGs from the sera of patients with multiple sclerosis

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“…The plasma samples were frozen in liquid nitrogen and lyophilized. All powders (7-10 mg per analysis) were analysed for metal composition by two-jet arc plasmatron atomic emission according to (Yudelevich et al, 1984;Shelpakova et al, 2002). Powders of lyophilized sodium citrate solutions (0.8%) were used as controls.…”

Section: Preparation Of the Rat Blood Plasma And Metal Content Analysismentioning

confidence: 99%

Oxidoreductase activities of polyclonal IgGs from the sera of Wistar rats are better activated by combinations of different metal ions

Tolmacheva

Zaksas

Buneva

et al. 2008

J of Molecular Recognition

106

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It was shown that IgGs purified from the sera of healthy Wistar rats contain several different bound Me2+ ions and oxidize 3,3'-diaminobenzidine through a H2O2-dependent peroxidase and H2O2-independent oxidoreductase activity. IgGs have lost these activities after removing the internal metal ions by dialysis against EDTA. External Cu2+ or Fe2+ activated significantly both activities of non-dialysed IgGs containing different internal metals (Fe > or = Pb > or = Zn > or = Cu > or = Al > or = Ca > or = Ni > or = Mn > Co > or = Mg) showing pronounced biphasic dependencies corresponding to approximately 0.1-2 and approximately 2-5 mM of Me2+, while the curves for Mn2+ were nearly linear. Cu2+ alone significantly stimulated both the peroxidase and oxidoreductase activities of dialysed IgGs only at high concentration (> or = 2 mM), while Mn2+ weakly activated peroxidase activity at concentration >3 mM but was active in the oxidoreductase oxidation at a low concentration (<1 mM). Fe2+-dependent peroxidase activity of dialysed IgGs was observed at 0.1-5 mM, but Fe2+ was completely inactive in the oxidoreductase reaction. Mg2+, Ca2+, Zn2+, Al2+ and especially Co2+ and Ni2+ were not able to activate dialysed IgGs, but slightly activated non-dialysed IgGs. The use of the combinations of Cu2+ + Mn2+, Cu2+ + Zn2+, Fe2+ + Mn2+, Fe2+ + Zn2+ led to a conversion of the biphasic curves to hyperbolic ones and in parallel to a significant increase in the activity as compared with Cu2+, Fe2+ or Mn2+ ions taken separately; the rates of the oxidation reactions, catalysed by non-dialysed and dialysed IgGs, became comparable. Mg2+, Co2+ and Ni2+ markedly activated the Cu2+-dependent oxidation reactions catalysed by dialysed IgGs, while Ca2+ inhibited these reactions. A possible role of the second metal in the oxidation reactions is discussed.

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“…The same amount of a control lyophilized powder was prepared from 25 ml of buffer B passed through the column free of Abs. All powders (5-7 mg per analysis) were used for metal composition analysis by two-jet arc plasmatron atomic emission according to Yudelevich et al (1984) and Shelpakova et al (2002). The mass percentage of metals (% of the powder containing all dried components of eluate) was determined.…”

Section: Purification Of Catalytic Iggmentioning

confidence: 99%

Metal ions-dependent peroxidase and oxidoreductase activities of polyclonal IgGs from the sera of Wistar rats

et al. 2006

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We present evidence showing that a small fraction of electrophoretically homogeneous IgGs from the sera of healthy Wistar rats is bound with several different Me2+ ions and oxidizes 3,3'-diaminobenzidine through a peroxidase activity in the presence of H2O2 and through an oxidoreductase activity in the absence of H2O2. During purification on Protein A-Sepharose and gel filtration, the polyclonal IgGs partially lose the Me2+ ions. Therefore, in the absence of external metal ions, the specific peroxidase activity of IgGs from the sera of different rats varied in the range 1.6-26% and increased up to 13-198% after addition of Fe2+ or Cu2+ ions as compared with horseradish peroxidase (HRP, taken for 100%). The oxidoreductase activity of HRP is 24-fold lower than its peroxidase activity, while oxidoreductase and peroxidase activities of IgGs are comparable. Oxidoreductase activities of different IgGs in the absence of external metal ions varied from 22 to 800%, and in the presence of Fe2+ or Cu2+ ions, from 37 to 1100% in comparison with the HRP oxidoreductase activity (100%). Chromatography of the IgGs on Chelex-100 leads to the adsorption of a small IgG fraction bound with metal ions and to its separation to many different subfractions demonstrating various affinities to the chelating resin and increased levels of the specific oxidoreductase and peroxidase activities. Antioxidant enzymes such as superoxide dismutases, catalases, and glutathione peroxidases are known to represent critical defense mechanisms for preventing oxidative modifications of DNA, proteins, and lipids. Peroxidase and oxidoreductase activity of antibodies may play an important role in the protection of organisms from oxidative stress and toxic compounds.

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A two-jet plasmatron for the spectrochemical analysis of geological samples

Cited by 32 publications

References 4 publications

Metal-dependent hydrolysis of myelin basic protein by IgGs from the sera of patients with multiple sclerosis

Metal-dependent hydrolysis of myelin basic protein by IgGs from the sera of patients with multiple sclerosis

Oxidoreductase activities of polyclonal IgGs from the sera of Wistar rats are better activated by combinations of different metal ions

Metal ions-dependent peroxidase and oxidoreductase activities of polyclonal IgGs from the sera of Wistar rats

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