A two-stage modification method using 1,4-α-glucan branching enzyme lowers the in vitro digestibility of corn starch

Antidiabetic bio-peptides of soft and hard wheat glutens

“…Pino (2020) mentioned that trypsin can cleave the Tenebrio Molitor protein to the smaller peptides (0.8–2 kDa) with α-glucosidase inhibitory capability . Ren et al (2020) also stated that alcalase can release the hemp protein hydrolysates (≤1 kDa) with the highest degree of α-glucosidase inhibition compared to the other enzymes (i.e., trypsin, fluorescein, neutralase, and papain). OsegueraToledo, GonzalezMejia, Sivaguru, and Amaya-Llano (2016) , who isolated the peptides from peeled beans, also suggested that the fine fractions (<1 kDa) expressed a good inhibitory (76.4 ± 0.5%) effect on α-glucosidase.…”

“…Food products that contain glycosidic bonds, such as carbohydrates and starches, are hydrolyzed more rapidly by α-amylase (1 , 4, D-glucan glucanohydrolase). α-amylase inhibitors usually slow down the hydrolysis of (α-1,4) glycosidic bonds, delay the digestion of carbohydrates, and lower the blood glucose level after a meal, by slowing down the digestion of carbohydrates ( Ren et al, 2020 ). In this study, the inhibitory activity of α-amylase was evaluated as a potential indicator of the antidiabetic response.…”

Novel Short-Clustered Maltodextrin as a Dietary Starch Substitute Attenuates Metabolic Dysregulation and Restructures Gut Microbiota in db/db Mice

“…8,9 In comparison with normal starch molecules, SCMD molecules display a higher branching density and are rich in short branch chains (polymerized by 2−6 glucose units). This short-clustered structure equips SCMD with reduced digestibility, 10 antiretrogradation property, 8 and enhanced solution stability. 11 These properties allow SCMD to serve as a potential starch substitute.…”

“…Recombinant GBE from G. thermoglucosidans STB02 was produced as previously described. 12 synthesized as previously detailed, 10 while pregelatinized starch (PGS) was produced using the same procedure without GBE treatment.…”

“…Therefore, the GBE-catalyzed reassembly of α-1,4 and α-1,6 glycosidic bonds in starch molecules is responsible for the dramatic reduction in digestibility. 10 Here, our results strongly supported the potential benefits of this molecular reassembly in regulating hyperglycemia of db/db mice. Many previous studies have demonstrated the beneficial effects of poorly digestible starches, which are primarily referred to as resistant starch (RS), on diabetes management.…”