A two-step serum-free culture system supports development of human oocytes from primordial follicles in the presence of activin

Telfer, Evelyn E.; McLaughlin, Marie; Ding, Christina; Thong, K.J.

doi:10.1093/humrep/den070

Cited by 417 publications

(513 citation statements)

References 41 publications

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“…For these patients, who run the potential risk of having malignant cells in their cryopreserved ovarian tissue, other options could be of interest, such as follicle culture with in vitro maturation [41,42] or grafting of isolated follicles enzymatically purified from frozen-thawed ovarian tissue [12,13,45].…”

Section: Resultsmentioning

confidence: 99%

Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study

Luyckx

Durant

Camboni

et al. 2013

J Assist Reprod Genet

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Section: Resultsmentioning

confidence: 99%

Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study

Luyckx

Durant

Camboni

et al. 2013

J Assist Reprod Genet

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“…However, based on the nature of the human ovarian tissue (more than 90% of the follicles are at primordial but not at pre-antral stage) and the prolonged follicle developmental period (Gougeon 1996), further experiments to improve the In vitro culture of pre-antral follicles efficiency of in vitro follicle culture and oocyte maturation are still needed, especially the establishment of a successful primordial follicle isolation and in vitro culture system to achieve higher success rates for future clinical application. The two-stage in vitro culture strategies recently described in the mouse (Jin et al 2010b) using a three-dimensional hydrogel matrix for secondary follicles, which produced a high rate of MII oocytes with over 50% of fertilized oocytes progressing to two-cell embryos, and in the human (Telfer et al 2008), in which exposure to activin resulted in 30% of primordial/primary follicles showing normal morphology, intact oocytes, and antral formation at the end of a 10-day culture period, are promising directions for future experimental and clinical approaches.…”

Section: Discussionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. In this study, we examined the viability and function of oocytes derived in vitro from pre-antral follicles as an alternative method for restoring fertility. Pre-antral follicles (specified as secondary follicle with a diameter around 100-130 mm) were mechanically isolated from vitrified-warmed and fresh adult mouse ovarian tissues and cultured for 12 days followed by an ovulation induction protocol at the end of this period to initiate oocyte maturation. Oocytes were then released from these follicles, fertilized in vitro, and cultured to the blastocyst stage and vitrified. After storage in liquid nitrogen for 2 weeks, groups of vitrified blastocysts were warmed and transferred into pseudo-pregnant recipient females. Although most of the isolated mouse pre-antral follicles from fresh (79.4%) and vitrified (75.0%) ovarian tissues survived the 12-day in vitro culture period, significantly fewer mature oocytes developed from vitrifiedwarmed pre-antral follicles than from the fresh controls (62.2 vs 86.4%, P!0.05). No difference was observed in embryo cleavage rates between these two groups, but the proportion of embryos that developed into blastocysts in the vitrification group was only half that of the controls (24.2 vs 47.2%, P!0.05). Nevertheless, live births of healthy normal pups were achieved after transfer of vitrified blastocysts derived from both experimental groups. This study shows that successful production of healthy offspring using an in vitro follicle culture system is feasible, and suggests that this procedure could be used in cancer patients who wish to preserve their fertility using ovarian tissue cryopreservation.

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“…In this case, isolation of primordial follicles could be carried out, as described by Dolmans et al [12], with the ultimate goal of recreating an artificial ovary [2]. Another option to avoid the risk of malignant cell transmission is to perform in vitro maturation from primordial follicles to antral follicles, as investigated by Telfer et al [60]. These options are always explained to leukemia patients at high risk of malignant involvement of the ovary [13].…”

Section: Risk Of Malignant Cells In Cryopreserved Ovarian Tissuementioning

confidence: 99%

A review of 15 years of ovarian tissue bank activities

Dolmans

Jadoul

Gilliaux

et al. 2013

J Assist Reprod Genet

109

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Purpose To review 15 years of activities in ovarian tissue cryobanking from medical database files, including patient indications, histological evaluation and clinical characteristics. Methods Retrospective longitudinal analysis of data from an ovarian tissue bank in an academic hospital. Five hundred and eighty-two patients had their ovarian tissue cryobanked between April 1997 and January 2012. Analysis of cryobanking database: precryopreservation patient characteristics, indications and safety issues, laboratory files and postcryopreservation clinical data. Results Of the 582 patients who had their ovarian tissue cryopreserved, 106 patients donated for research purposes and 476 patients for fertility preservation and long-term cryopreservation. Clinical data analysis of the 476 patients revealed a mean age at the time of cryopreservation of 23± 8.5 years (range: 9 months -39 years), with 96.2 % of subjects aged ≤35 years (n=458). Among 391 cases of malignant disease, hematological malignancies (39.9 %, n=156) and breast cancer (21.7 %, n=85) were the two main indications.At histology, malignant cells were found in ovarian tissue from leukemia patients (n = 3) and non-Hodgkin's lymphoma patients (n=2). Eleven patients underwent autotransplantation, resulting in 5 live births and 1 ongoing pregnancy. Conclusion This is the largest and most comprehensive study to describe and analyze indications and clinical patient characteristics before and after ovarian tissue cryopreservation. The procedure is safe, easy and promising. The database concept is a useful tool in patient selection for autotransplantation.

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A two-step serum-free culture system supports development of human oocytes from primordial follicles in the presence of activin

Abstract: The results reported here demonstrate that under certain conditions, it is possible to achieve accelerated oocyte/follicle development from human primordial/primary follicles. This provides the first encouraging step towards achieving full in vitro growth of human oocytes.

Cited by 417 publications

References 41 publications

Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study

Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

A review of 15 years of ovarian tissue bank activities

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