A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production

Wang, Meng; Yang, Chen; François, Jean; Wan, Xia; Deng, Qianchun; Feng, Danyang; Deng, Shiyu; Chen, Shouwen; Huang, Fenghong; Chen, Wenchao; Gong, Yiqin

doi:10.3389/fbioe.2021.735714

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…Compared with the antibacterial activity of surfactin, iturin A is mainly fungicidal, and the amino acid composition of iturin A is complex. Meanwhile, iturin A yield produced by Bacillus is relatively low, although several pieces of research on fermentation process optimization have been conducted to improve its yield (Jin et al, 2015;Wang et al, 2021). Previously, by replacing the promoter of the iturin A synthetase cluster and deleting the negative regulator gene abrB in B. amyloliquefaciens, iturin A yield was increased by 1.81-fold (Xu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The low synthesis capability of iturin A limits its application promotion, although several approaches of genetic engineering breeding and fermentation process optimization have been developed to improve iturin A production Jin et al, 2015;Zhang et al, 2017;Shi et al, 2018;Dang et al, 2019;Xu et al, 2020;Wang et al, 2021). To improve iturin A synthesis, fatty acid biosynthetic pathways were systematically strengthened in Bacillus amyloliquefaciens HZ-12, a wide-type strain with the synthesis capability of iturin A (Xu et al, 2020), and the synthesis modules of malonyl-CoA, malonyl-ACP, free fatty acid, and fatty acid-CoA were strengthened for iturin A production (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

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Enhanced production of iturin A by strengthening fatty acid synthesis modules in Bacillus amyloliquefaciens

Gao

She

Shi

et al. 2022

Front. Bioeng. Biotechnol.

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Iturin A is a biosurfactant with various applications, and its low synthesis capability limits its production and application development. Fatty acids play a critical role in cellular metabolism and target product syntheses, and the relationship between fatty acid supplies and iturin A synthesis is unclear. In this study, we attempted to increase iturin A production via strengthening fatty acid synthesis pathways in Bacillus amyloliquefaciens. First, acetyl-CoA carboxylase AccAD and ACP S-malonyltransferase fabD were overexpressed via promoter replacement, and iturin A yield was increased to 1.36 g/L by 2.78-fold in the resultant strain HZ-ADF1. Then, soluble acyl-ACP thioesterase derived from Escherichia coli showed the best performance for iturin A synthesis, as compared to those derived from B. amyloliquefaciens and Corynebacterium glutamicum, the introduction of which in HZ-ADF1 further led to a 57.35% increase of iturin A yield, reaching 2.14 g/L. Finally, long-chain fatty acid-CoA ligase LcfA was overexpressed in HZ-ADFT to attain the final strain HZ-ADFTL2, and iturin A yield reached 2.96 g/L, increasing by 6.59-fold, and the contents of fatty acids were enhanced significantly in HZ-ADFTL2, as compared to the original strain HZ-12. Taken together, our results implied that strengthening fatty acid supplies was an efficient approach for iturin A production, and this research provided a promising strain for industrial production of iturin A.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced production of iturin A by strengthening fatty acid synthesis modules in Bacillus amyloliquefaciens

Gao

She

Shi

et al. 2022

Front. Bioeng. Biotechnol.

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“…These seed meals are known either for their elevated polyphenolic concentrations, such as GSM, SBSM, and RSM or for their content in polyphenolic compounds with a strong antimicrobial and anti-inflammatory effect. Thus, they are promising agro-industrial byproducts to manage the piglet's weaning crisis (Tabasco et al 2011;Bouarab-Chibane et al 2019;Grosu et al 2019;Wang et al 2021;Czech et al 2022;Taranu et al 2022;Wang et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial potential of polyphenols obtained from agro-industrial by-products

Anghel,

Țăranu

2023

Archiva Zootechnica

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Antibiotics and zinc oxide are therapeutic agents widely used in the livestock industries, reducing significantly the negative effects of weaning by improving the growth rate and decreasing mortality. Although, due to the potential public and environmental risks associated with antibiotic resistance and soil pollution their use in the E.U. has been drastically reduced or banned, thus, alternative solutions been necessary to be develop. The addition of polyphenols to animal feed has shown promising results, as they have a broad antimicrobial activity including against E. coli F4 (K88). Moreover, it has been observed to hold a wide range of beneficial properties such as antidiarrhoeic, anti-inflammatory, and antioxidant activity. In the present study in vitro experiments were performed in order to investigate the antimicrobial activity of several polyphenolic extracts obtained from mustard seed meals of two origins (Poland and Moldavia), grape seed meal, sea buckthorn seed meal, rapeseed meal, and rapeseed meal fermented with S. cerevisiae, against E. coli F4 (K88) proliferation measured at different intervals for 24 hours. The results revealed that polyphenols have a considerable antimicrobial effect in high concentrations. The inhibition was dependent also on polyphenol composition and even the origin place. Withal, it was observed that the polyphenols extracted from rapeseed meal and fermented rapeseed meal presented noticeable differences in inhibition, in similar concentrations, this suggesting that the fermentation process considerably affects the profile of rapeseed meal polyphenols. Likewise for the Moldavian mustard seed extract which significantly inhibited bacterial growth from very low concentrations although it had a low concentration of total polyphenols. Thus, the polyphenolic content of fermented rape seed meal extract presented an enhanced antimicrobial activity against E. coli F4 (K88) compared with the non-fermented rape seed meal extract. Although the total polyphenol concentration was lower in FRSM than in RSM. Polyphenols extracted from Moldavia mustard seed meal exhibited remarkable efficacy in inhibiting bacterial growth. Notably, even at a concentration as low as 3.71 µg/ml, the polyphenols demonstrated significant antimicrobial activity, effectively combating bacterial development. Overall, our study demonstrated that polyphenols represent a promising substitute for antibiotics and zinc oxide and might represent an encouraging long-term solution in overcoming the weaning crisis in pigs. In vivo experimentations are needed to validate these results.

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“…Phenolic compounds of L. barbarum remain unused after the extraction of oil by pressing or solution extraction. Therefore, the extraction, identification, and quantification of active components in meals are important for the comprehensive exploitation of meals and the improvement of value-added by-products (Malešević et al, 2014;Wang et al, 2021). The embedded phenolic compounds hold promising nutritional and health benefits which can be exploited through suitable extraction methods.…”

Section: Introductionmentioning

confidence: 99%

HPLC-MS/MS targeting analysis of phenolics metabolism and antioxidant activity of extractions from Lycium barbarum and its meal using different methods

et al. 2022

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The phenolic compounds in dried Lycium barbarum (LB) and L. barbarum meal (LBM) were extracted using ultrasonic method, reflux method and enzymatic method, and the composition, content of polyphenols and antioxidant abilities of the extracts were analyzed. 40 phenolic compounds were identified with HPLC-MS/MS, with 35 compounds detected from LB and 33 from LBM. 40 compounds were classified into phenolic acids and their derivatives, flavonoids, and their derivatives, lignanamides and other phenolic substances. Differences existed in samples extracted from different materials or under different extraction methods. It was explicitly observed that LB was higher than LBM in phenolic content. The phenolics in LB and LBM had significant antioxidant effects, with LBM extracts showing the higher antioxidant effect. This study compared the differences in polyphenols of L. barbarum and its meal, meanwhile explored the effects of different extraction methods, which could contribute to the exploitation of L. barbarum and its meal.

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A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production

Cited by 7 publications

References 38 publications

Enhanced production of iturin A by strengthening fatty acid synthesis modules in Bacillus amyloliquefaciens

Enhanced production of iturin A by strengthening fatty acid synthesis modules in Bacillus amyloliquefaciens

Antimicrobial potential of polyphenols obtained from agro-industrial by-products

HPLC-MS/MS targeting analysis of phenolics metabolism and antioxidant activity of extractions from Lycium barbarum and its meal using different methods

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