A ubiquitous protein is the source of naturally occurring peptides that are recognized by a CD8+ T-cell clone.

Udaka, Keiko; Tsomides, Theodore J.; Walden, Peter; Fukusen, Naomi; Eisen, Herman N.

doi:10.1073/pnas.90.23.11272

Cited by 120 publications

(86 citation statements)

References 28 publications

(24 reference statements)

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“…This foreign MHC/ peptide combination mimics self-MHC plus foreign peptide. Numerous studies with MHC class I-restricted CTL support this hypothesis (5,6,(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27). Although some alloreactive T cells had a strict requirement for the type of peptide that associates with the foreign MHC (24 -27), most alloreactive CTL displayed substantial degeneracy for peptide recognition (19,(21)(22)(23).…”

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confidence: 98%

The Alloreactive and Self-Restricted CD4+ T Cell Response Directed Against a Single MHC Class II/Peptide Combination

Kovalik

Singh

Mendiratta

et al. 2000

The Journal of Immunology

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The cellular basis for allograft rejection derives from the strong T cell response to cells bearing foreign MHC. While it was originally assumed that alloreactive T cells focus their recognition on the polymorphic residues that differ between syngeneic and allogeneic MHC molecules, studies with MHC class I-restricted CTL have shown that MHC-bound peptides play a critical role in allorecognition. It has been suggested that alloreactive T cells depend more strongly on interactions with the MHC molecule than with the associated peptide, but there is little evidence to support this idea. Here we have studied the alloreactive and self-restricted response directed against the class II H2-Ab molecule bound with a single peptide, Ep, derived from the H2-Eα chain. This MHC class II-peptide combination was a poor target and stimulator of alloreactive CD4+ T cell responses, indicating that MHC-bound peptides are as important for alloreactive CD4+ T cells as they are for alloreactive CTL. We also generated alloreactive T cells with exquisite specificity for the Ab/Ep complex, and compared their reactivity with self-restricted T cells specific for the same Ab/Ep complex. Our results showed that peptide-specific alloreactive T cells, as compared with self-restricted T cells, were more sensitive to peptide stimulation, but equally sensitive to amino acid substitutions in the peptide. These findings indicate that alloreactive and self-restricted T cells interact similarly with their MHC/peptide ligand.

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confidence: 98%

The Alloreactive and Self-Restricted CD4+ T Cell Response Directed Against a Single MHC Class II/Peptide Combination

Kovalik

Singh

Mendiratta

et al. 2000

The Journal of Immunology

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“…Interestingly, C terminal to the H4 b epitope is a stretch of three basic arginine-lysine-arginine residues in the Emp-3 sequence (30). Peptides longer than the processed epitopes are known to bind MHC class I molecules (42,43) and be recognized by CTL (44). Therefore, it is conceivable that M11 recognizes a longer peptide precursor in addition to the processed P H4 b epitope.…”

Section: Discussionmentioning

confidence: 99%

The H4b Minor Histocompatibility Antigen Is Caused by a Combination of Genetically Determined and Posttranslational Modifications

Yadav

Yoshimura

Boesteanu

et al. 2003

The Journal of Immunology

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Minor histocompatibility (H) Ag disparities result in graft-vs-host disease and chronic solid allograft rejection in MHC-identical donor-recipient combinations. Minor H Ags are self protein-derived peptides presented by MHC class I molecules. Most arise as a consequence of allelic variation in the bound peptide (p) that results in TCR recognizing the p/MHC as foreign. We used a combinational peptide screening approach to identify the immune dominant H2Kb-restricted epitope defining the mouse H4b minor H Ag. H4b is a consequence of a P3 threonine to isoleucine change in the MHC-bound peptide derived from epithelial membrane protein-3. This allelic variation also leads to phosphorylation of the H4b but not the H4a epitope. Further, ex vivo CD8+ T lymphocytes bind phosphorylated Ag tetramers with high efficiency. Although we document the above process in the minor H Ag system, posttranslational modifications made possible by subtle amino acid changes could also contribute to immunogenicity and immune dominance in tumor immunotherapeutic settings.

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“…The data were analysed by using Lysis II version1. [33] were prepared on a Milli Gen 9050 Pep Synthesizer (Burlington, MA) and purified by HPLC to >95% homogeneity. These peptides were tested by using the method of Lie et al [34] …”

Section: Methodsmentioning

confidence: 99%

Interaction of 2C T Cells with a Hybrid L^d Molecule Bearing an α3 Domain Derived from the Class Ib Molecule, Qa‐2

Ungchusri

Forman

1997

Scand J Immunol

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Thus, either CD8 is not required for negative selection of this TCR or a weak interaction of CD8 with L Q3 is sufficient. TSA-1, a developmentally regulated marker, was used to follow the process of negative selection. The results show that deletion of 2C T cells does not occur until thymocytes reach the double positive (DP) stage. Furthermore, the authors noted a small population of DP TSA-1 hi cells remains, while DP TSA-1 int and TSA-1 lo cells are absent. These data support the notion that thymocytes either reach a particular stage of development or locate in an appropriate intrathymic compartment before they undergo negative selection.

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A ubiquitous protein is the source of naturally occurring peptides that are recognized by a CD8+ T-cell clone.

Abstract: A ubiquitous protein is the source of naturally occurring peptides that are recognized by a CD8+ T-cell clone (T-ceil ABSTRACTWe previously isolated from mouse spleen an octapeptide (LSPFPFDL) that in association with the class I major histocompatibiity complex protein Ld is recognized by

Cited by 120 publications

References 28 publications

The Alloreactive and Self-Restricted CD4+ T Cell Response Directed Against a Single MHC Class II/Peptide Combination

The Alloreactive and Self-Restricted CD4+ T Cell Response Directed Against a Single MHC Class II/Peptide Combination

The H4b Minor Histocompatibility Antigen Is Caused by a Combination of Genetically Determined and Posttranslational Modifications

Interaction of 2C T Cells with a Hybrid L^d Molecule Bearing an α3 Domain Derived from the Class Ib Molecule, Qa‐2

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A ubiquitous protein is the source of naturally occurring peptides that are recognized by a CD8+ T-cell clone.

Abstract: A ubiquitous protein is the source of naturally occurring peptides that are recognized by a CD8+ T-cell clone (T-ceil ABSTRACTWe previously isolated from mouse spleen an octapeptide (LSPFPFDL) that in association with the class I major histocompatibiity complex protein Ld is recognized by

Cited by 120 publications

References 28 publications

The Alloreactive and Self-Restricted CD4+ T Cell Response Directed Against a Single MHC Class II/Peptide Combination

The Alloreactive and Self-Restricted CD4+ T Cell Response Directed Against a Single MHC Class II/Peptide Combination

The H4b Minor Histocompatibility Antigen Is Caused by a Combination of Genetically Determined and Posttranslational Modifications

Interaction of 2C T Cells with a Hybrid Ld Molecule Bearing an α3 Domain Derived from the Class Ib Molecule, Qa‐2

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Interaction of 2C T Cells with a Hybrid L^d Molecule Bearing an α3 Domain Derived from the Class Ib Molecule, Qa‐2