A UHPLC-Q-TOF-MS-based serum and urine metabolomics approach reveals the mechanism of Gualou-Xiebai herb pair intervention against atherosclerosis process in ApoE-/- mice

Wang, Yuting; Sun, Xingming; Qiu, Jingwen; An, Zhenmei; Xu, Peng-Bo; Liu, Yarong; Wu, Hongfei

doi:10.1016/j.jchromb.2022.123567

Cited by 8 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glycerophospholipids regulate inflammation, immunity, and tumor development [ 18 , 19 ] and were shown to be effective guardians of intestinal epithelial homeostasis [ 20 , 21 ]. Glycerophospholipid metabolism plays a key pathogenic role in the occurrence and progression of atherosclerosis [ 22 ]. This study found that there were significant changes in the levels of phosphatidylcholine (PC) and lysophosphatidylcholine (LysoPC) in the intestines of SR−B1 knockout mice.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Intestinal Metabolites in SR−B1 Knockout Mice via Ultra−Performance Liquid Chromatography Quadrupole Time−of−Flight Mass Spectrometry

et al. 2023

View full text Add to dashboard Cite

Scavenger receptor class B type 1 (SR−B1), a multiligand membrane receptor, is expressed in a gradient along the gastrocolic axis. SR−B1 deficiency enhances lymphocyte proliferation and elevates inflammatory cytokine production in macrophages. However, whether SR−B1 affects intestinal metabolites is unclear. In this study, we detected metabolite changes in the intestinal tissue of SR−B1−/− mice, including amino acids and neurotransmitters, by ultra−performance liquid chromatography quadrupole time−of−flight mass spectrometry (UHPLC−Q−TOF/MS) and HPLC. We found that SR−B1−/− mice exhibited changes in intestinal lipid metabolites and metabolic pathways, including the glycerophospholipid, sphingolipid, linoleic acid, taurine, and hypotaurine metabolic pathways. SR−B1 deficiency influenced the contents of amino acids and neurotransmitters in all parts of the intestine; the contents of leucine (LEU), phenylalanine (PHE), tryptophan (TRP), and tyrosine (TYR) were affected in all parts of the intestine; and the contents of 3,4−dihydroxyphenylacetic acid (DOPAC) and dopamine (DA) were significantly decreased in both the colon and rectum. In summary, SR−B1 deficiency regulated intestinal lipids, amino acids, and neurotransmitter metabolism in mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of Intestinal Metabolites in SR−B1 Knockout Mice via Ultra−Performance Liquid Chromatography Quadrupole Time−of−Flight Mass Spectrometry

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The present study describes the application of an orthogonal projection of MSOP for investigating in vivo metabolism of Oroxylum indicum (L.) Kurz (seed of Oroxylum indicum (L.) Kurz) [ [24] , [25] , [26] ]. The theory and mathematical algorithm underlying MSOP are presented, along with its successful implementation for rapid screening and characterization of flavonoid metabolites in Oroxylum indicum (L.) Kurz.…”

Section: Introductionmentioning

confidence: 99%

The mysteries of pharmacokinetics and in vivo metabolism of Oroxylum indicum (L.) Kurz: A new perspective from MSOP method

Zhang,

Wang

et al. 2024

Heliyon

View full text Add to dashboard Cite

“…The initiation of AS results from the accumulation of plaque in the sub-endothelial space of arteries, leading to insufficient blood flow and oxygen delivery to vital organs [2,3] It is also characterized as a chronic, multifactorial, and progressive disease showing notable variations in metabolites during different pathological stages [4]. At the initial stage of AS, metabolic imbalances such as a decrease in glucose and glycine and an increase in low-density lipoprotein (LDL) cholesterol may be observed, but without any clear clinical symptoms [5]. As the disease progresses, the narrowing of the arteries limits the blood flow, resulting in symptoms such as hypertension, headaches, and other clinical symptoms.…”

Section: Introductionmentioning

confidence: 99%

“…In the meantime, the body may also experience changes in the metabolism of amino acids, phenylalanine, and methionine [4]. In the advanced stage of Metabolites 2023, 13, 1160 2 of 14 AS, the plaque becomes unstable and ruptures, leading to thrombosis formation, along with imbalances in the metabolism of sugars, fatty acids, amino acids, choline, and cholesterol [5]. Despite scientific discoveries and significant progress in the treatment of AS, the disease remains a major killer globally [4].…”

Section: Introductionmentioning

confidence: 99%

Identification of Novel Biomarkers for Early Diagnosis of Atherosclerosis Using High-Resolution Metabolomics

Sardar,

Nam,

Kim

et al. 2023

Metabolites

View full text Add to dashboard Cite

Atherosclerosis (AS) is a metabolic disorder and the pre-stage of several cardiovascular diseases, including myocardial infarction, stroke, and angina pectoris. Early detection of AS can provide the opportunity for effective management and better clinical results, along with the prevention of further progression of the disease. In the current study, an untargeted and targeted metabolomic approach was used to identify possible metabolic signatures that have altered levels in AS patients. A total of 200 serum samples from individuals with AS and normal were analyzed via liquid chromatography–high-resolution mass spectrometry. Univariate and multivariate analysis approaches were used to identify differential metabolites. A group of metabolites associated with bile acids, amino acids, steroid hormones, and purine metabolism were identified that are capable of distinguishing AS-risk sera from normal. Further, the targeted metabolomics approach confirmed that six metabolites, namely taurocholic acid, cholic acid, cortisol, hypoxanthine, trimethylamine N-oxide (TMAO), and isoleucine, were found to be significantly upregulated, while the concentrations of glycoursodeoxycholic acid, glycocholic acid, testosterone, leucine, methionine, phenylalanine, tyrosine, and valine were found to be significantly downregulated in the AS-risk sera. The receiver operating characteristic curves of three metabolites, including cortisol, hypoxanthine, and isoleucine, showed high sensitivity and specificity. Taken together, these findings suggest cortisol, hypoxanthine, and isoleucine as novel biomarkers for the early and non-invasive detection of AS. Thus, this study provides new insights for further investigations into the prevention and management of AS.

show abstract

A UHPLC-Q-TOF-MS-based serum and urine metabolomics approach reveals the mechanism of Gualou-Xiebai herb pair intervention against atherosclerosis process in ApoE-/- mice

Cited by 8 publications

References 47 publications

Analysis of Intestinal Metabolites in SR−B1 Knockout Mice via Ultra−Performance Liquid Chromatography Quadrupole Time−of−Flight Mass Spectrometry

Analysis of Intestinal Metabolites in SR−B1 Knockout Mice via Ultra−Performance Liquid Chromatography Quadrupole Time−of−Flight Mass Spectrometry

The mysteries of pharmacokinetics and in vivo metabolism of Oroxylum indicum (L.) Kurz: A new perspective from MSOP method

Identification of Novel Biomarkers for Early Diagnosis of Atherosclerosis Using High-Resolution Metabolomics

Contact Info

Product

Resources

About