In this study, nitrogen and sulfur codoped carbon quantum
dot (N,S-CQD)
fluorescent nanoprobes were synthesized through a one-step hydrothermal
method using l-cysteine and α-methacrylic acid as raw
materials, and an “on–off–on” fluorescence
mechanism was designed using N,S-CQDs for sequential determination
of Fe3+ and H2S in human serum. The quenching
process (on–off) and mechanism of N,S-CQDs by Fe3+ were studied via fluorescence spectroscopy, zeta potential, cyclic
voltammetry, and orbital energy level simulation. Meanwhile, an “off–on”
strategy for the N,S-CQD/Fe3+ fluorescence sensing platform
was constructed for the determination of the H2S-based
“on–off” mechanism. This study not only achieved
highly selective detection of Fe3+ and H2S over
a wide concentration range of 0–250, 250–500, and 2.5–900
μM, respectively, but also improved our understanding on the
specific identifications between N,S-CQDs, Fe3+, and H2S. What is more, it was found that N,S-CQDs also possessed
pH and temperature sensing performance. Finally, N,S-CQD fluorescent
nanoprobes were successfully applied for the determination of Fe3+ and H2S in spiked serum samples, which also proved
their potential application in medical diagnosis and biosensors.