A unique miR775-GALT9 module regulates leaf senescence in Arabidopsis during post-submergence recovery by modulating ethylene and the abscisic acid pathway

Abstract

Submergence-induced hypoxic condition negatively affects the plant growth and development, and causes early onset of senescence. Hypoxia alters the expression of a number of microRNAs (miRNAs). However, the molecular function of submergence stress-induced miRNAs in physiological or developmental changes and recovery remains poorly understood. Here we show that miR775 is an Arabidopsis thaliana-specific young and unique miRNA that possibly evolved non-canonically. miR775 post-transcriptionally regulates Galactosyltransferase (GALT9) and their expression is inversely affected at 24 hours of complete submergence stress. The overexpression of miR775 (miR775-Oe) confers enhanced recovery from submergence stress and reduced accumulation of RBOHD and ROS, in contrast to wild type and MIM775 Arabidopsis shoot. A similar recovery phenotype of galt9 mutant indicates the role of miR775-GALT9 module in post-submergence recovery. We predicted Golgi-localized GALT9 to be potentially involved in protein glycosylation. The altered expression of senescence-associated genes (SAG12, SAG29, and ORE1), ethylene signalling (EIN2 and EIN3) and ABA biosynthesis (NCED3) pathway genes in miR775-Oe, galt9 and MIM775 plants. Thus, our results indicate the role of miR775-GALT9 module in post-submergence recovery through a crosstalk with ethylene and ABA pathway.

Identification and Expression Analysis of the Nucleotidyl Transferase Protein (NTP) Family in Soybean (Glycine max) under Various Abiotic Stresses

“…Moreover, research has revealed that gma-miR172c influences the tolerance of soybean roots to stress by affecting the target gene NNC1 (Nodule Number Control 1) [44]. Many small RNAs have been experimentally confirmed to play a role in plant responses to abiotic stresses [5,[45][46][47][48]. NTP, as an enzyme that has the function of terminal modification, plays an important role in the process of sRNA biogenesis and degradation [8,23].…”

Reactive Oxygen Species: A Crosslink between Plant and Human Eukaryotic Cell Systems

“…Melatonin MT inhibits age-dependent and stress-induced leaf senescence in cucumber by activating the antioxidant system and the IAA synthesis and signaling pathway, while simultaneously inhibiting the ABA synthesis and signaling pathways, thereby reducing leaf senescence [99]. The miR775-GALT9 module regulates leaf senescence in Arabidopsis through crosstalk between the ethylene signaling and ABA biosynthetic pathways during post-submergence recovery [100].…”

Serotonin: A frontline player in plant growth and stress responses

“…Previously, miRNAs have been implicated in various aspects of plant growth and development, including several abiotic and biotic stresses (Gautam et al, 2017, 2021; Mishra et al, 2022, 2023; Mishra & Maurya, 2023; Panda et al, 2022; Singh et al, 2018, 2020, 2021; Vivek & Kumar, 2021; Yadav et al, 2020). In animal systems, it is well known that miRNAs regulate the accumulation of serotonin by modulating various genes (Arai et al, 2013; Kassan et al, 2022; McKibben & Dwivedi, 2021; Zhao et al, 2019).…”