A Unique Preliminary Study on Placental Apoptosis in Mice with Passive Immunization of Anti-Phosphatidylethanolamine Antibodies and Anti-Factor XII Antibodies

Abstract

Mice with passive immunization of aPE (or anti-LDC27) and aFXII (or anti-IPP30) produced a slight increase in fetal resorption, but markedly induced thrombosis and hemorrhage in the placenta associated with lower platelet counts and increased placental apoptosis. In addition, fewer mitotic cells, less trophoblast giant cell invasion, and more shrunken cells in the deciduas were seen. Our study supports the pathogenic role of aPE and aFXII in pregnancy complications and also suggests a beneficial role of LDC27 and IPP30 antigens on pregnancy failures.

Autoantibodies to Factor XII and Kininogen‐Dependent Antiphosphatidylethanolamine Antibodies in Patients with Recurrent Pregnancy Loss Augment Platelet Aggregation

“…Recently, Velayuthaprabhu et al 31 reported a murine model in which infusion of aPE, anti-LDC27, anti-FXII, and anti-IPP30 induced thrombosis and hemorrhage in the placenta and increased placental apoptosis. Moreover, kininogen-independent IgG aPE did not affect thrombin-induced platelet aggregation.…”

“…22,31 Two peptides, LDC27 and IPP30 (Table I), were synthesized. 22,31 Two peptides, LDC27 and IPP30 (Table I), were synthesized.…”

Beta-2 GPI induced tissue factor and placental apoptosis for the pathophysiology of pregnancy loss in antiphospholipid syndrome

“…11,15 A subsequent study in other pregnancy complications such as intra uterine growth restriction (IUGR), preeclampsia and fetal growth restriction (FGR) reported that a great incidence of apoptosis play a leading role in such complications.…”

“…Therefore, recent studies elucidated the impact of placental apoptosis in the aspect of placentation and pregnancy complications in autoimmune diseases like APS. 15 Based on the crucial role of TF and inflammation in aPL-induced pregnancy loss, it would be interesting to understand the mechanism of TF in contributing inflammation either by increased index of apoptosis in placenta for aPL-induced pregnancy loss.…”

The contribution of antiphosphatidylethanolamine antibodies in the diagnosis of the antiphospholipid syndrome

“…A murine model in which infusion of aPEs induced thrombosis and haemorrhage in the placenta supports the role of aPEs in obstetric complications of APS. 7 The relationship between aPEs and thrombosis has also been reported. [3][4][5] aPEs have been shown in 30% of patients with a history of stroke.…”