A unique Zn(II)2-Cys6-type protein, KpeA, is involved in secondary metabolism and conidiation in Aspergillus oryzae

Arakawa, Gen-ya; Kudo, Hayato; Yanase, Atsushi; Eguchi, Yuichi; Kodama, Haruka; Ogawa, Mitsuhiro; Koyama, Yasuji; Shindo, Hitoshi; Hosaka, Masaru; Tokuoka, Masafumi

doi:10.1016/j.fgb.2019.02.004

Cited by 27 publications

(20 citation statements)

References 63 publications

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“…This disruption library as well as the overexpression library which covers a part of transcription factor genes has greatly contributed to the identification of novel transcription factors responsible for hydrolytic gene expression, such as FlbC, ManR, and PrtR. Such libraries have also been effectively utilized to mine novel transcription factors, such as AtrR (azole drug resistance and ABC transporter gene regulation; Hagiwara et al, 2017 ), EcdR (conidiophore development; Jin et al, 2011 ), and KpeA (kojic acid production and conidiation; Arakawa et al, 2019 ), that are involved in the regulatory expression of genes other than hydrolytic genes. Since the library of disruption mutant strains contains over 500 genes putatively involved in transcriptional regulation, further identification of novel transcription factors that regulate the expression of hydrolytic genes in A. oryzae is highly expected.…”

Section: Discussionmentioning

confidence: 99%

Induction and Repression of Hydrolase Genes in Aspergillus oryzae

Tanaka

Gomi

2021

Front. Microbiol.

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The filamentous fungus Aspergillus oryzae, also known as yellow koji mold, produces high levels of hydrolases such as amylolytic and proteolytic enzymes. This property of producing large amounts of hydrolases is one of the reasons why A. oryzae has been used in the production of traditional Japanese fermented foods and beverages. A wide variety of hydrolases produced by A. oryzae have been used in the food industry. The expression of hydrolase genes is induced by the presence of certain substrates, and various transcription factors that regulate such expression have been identified. In contrast, in the presence of glucose, the expression of the glycosyl hydrolase gene is generally repressed by carbon catabolite repression (CCR), which is mediated by the transcription factor CreA and ubiquitination/deubiquitination factors. In this review, we present the current knowledge on the regulation of hydrolase gene expression, including CCR, in A. oryzae.

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Section: Discussionmentioning

confidence: 99%

Induction and Repression of Hydrolase Genes in Aspergillus oryzae

Tanaka

Gomi

2021

Front. Microbiol.

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“…For example, a novel Zn 2 Cys 6 transcription factor BcGaaR could regulate D-galacturonic acid utilization in Botrytis cinerea [37]. A unique Zn(II) 2 -Cys 6 -type protein, KpeA, was involved in secondary metabolism and conidiation in Aspergillus oryzae [38]. A functionally conserved Zn 2 Cys 6 transcription factor regulated necrotrophic effector gene expression and host-specific virulence of two major Pleosporales fungal pathogens of wheat [39].…”

Section: Discussionmentioning

confidence: 99%

ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum

Gao

Xiè

et al. 2020

Viruses

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We previously identified Sclerotinia sclerotiorum negative-stranded virus 1 (SsNSRV-1), the first (−) ssRNA mycovirus, associated with hypovirulence of its fungal host Sclerotinia sclerotiorum. In this study, functional analysis of Open Reading Frame Ι (ORF Ι) of SsNSRV-1 was performed. The integration and expression of ORF Ι led to defects in hyphal tips, vegetative growth, and virulence of the mutant strains of S. sclerotiorum. Further, differentially expressed genes (DEGs) responding to the expression of ORF Ι were identified by transcriptome analysis. In all, 686 DEGs consisted of 267 up-regulated genes and 419 down-regulated genes. DEGs reprogramed by ORF Ι were relevant to secretory proteins, pathogenicity, transcription, transmembrane transport, protein biosynthesis, modification, and metabolism. Alternative splicing was also detected in all mutant strains, but not in hypovirulent strain AH98, which was co-infected by SsNSRV-1 and Sclerotinia sclerotiorum hypovirus 1 (SsHV-1). Thus, the integrity of SsNSRV-1 genome may be necessary to protect viral mRNA from splicing and inactivation by the host. Taken together, the results suggested that protein ORF Ι could regulate the transcription, translation, and modification of host genes in order to facilitate viral proliferation and reduce the virulence of the host. Therefore, ORF Ι may be a potential gene used for the prevention of S. sclerotiorum.

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“…For example, A. oryzae extracellularly produces kojic acid (KA) as a secondary metabolite, which is used as a skin-lightening agent in cosmetics ( Figure 1 B) [ 70 ]. Although the KA biosynthetic processes are less understood, so far four KA biosynthesis-related genes—namely kojA , kojR, kojT and kpeA —were identified [ 71 , 72 ]. KojR and KpeA are Zn(II) 2 Cys 6 transcriptional activator and repressor, respectively, that are thought to regulate the expression of kojA and kojT genes, which putatively encode an enzyme and a transporter, respectively.…”

Section: Secretory Pathwaymentioning

confidence: 99%

Membrane Traffic in Aspergillus oryzae and Related Filamentous Fungi

Higuchi

2021

JoF

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The industrially important filamentous fungus Aspergillus oryzae, known as the yellow Koji mold and also designated the Japanese National fungus, has been investigated for understanding the intracellular membrane trafficking machinery due to the great ability of valuable enzyme production. The underlying molecular mechanisms of the secretory pathway delineate the main secretion route from the hyphal tip via the vesicle cluster Spitzenkörper, but also there is a growing body of evidence that septum-directed and unconventional secretion occurs in A. oryzae hyphal cells. Moreover, not only the secretory pathway but also the endocytic pathway is crucial for protein secretion, especially having a role in apical endocytic recycling. As a hallmark of multicellular filamentous fungal cells, endocytic organelles early endosome and vacuole are quite dynamic: the former exhibits constant long-range motility through the hyphal cells and the latter displays pleiomorphic structures in each hyphal region. These characteristics are thought to have physiological roles, such as supporting protein secretion and transporting nutrients. This review summarizes molecular and physiological mechanisms of membrane traffic, i.e., secretory and endocytic pathways, in A. oryzae and related filamentous fungi and describes the further potential for industrial applications.

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A unique Zn(II)2-Cys6-type protein, KpeA, is involved in secondary metabolism and conidiation in Aspergillus oryzae

Cited by 27 publications

References 63 publications

Induction and Repression of Hydrolase Genes in Aspergillus oryzae

Induction and Repression of Hydrolase Genes in Aspergillus oryzae

ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum

Membrane Traffic in Aspergillus oryzae and Related Filamentous Fungi

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