We study the translation invariant properties of the eigenvalues of scattering transmission problem. We examine the functional derivative of the eigenvalue density function Δ(̂) to the defining index of refraction ( ). By the limit behaviors in frequency sphere, we prove some results on the inverse uniqueness of index of refraction. In physics, Doppler's effect connects the variation of the frequency/eigenvalue and the motion velocity/variation of position variable. In this paper, we proved the functional derivative Δ(̂) = (1 + √ ( (̂)))/ .