A Universal and Facile Approach for the Formation of a Protein Hydrogel for 3D Cell Encapsulation

Chen, Yin; Dai, Xin; Huang, Lu; Sun, Yong; Chan, Ho Nam; Shen, Bo; Zeng, Xiaoqian; Wu, Zhenguo; Hsing, I‐Ming; Guo, Zhihong; Wu, Hongkai

doi:10.1002/adfm.201502942

Cited by 23 publications

(21 citation statements)

References 37 publications

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“…RGD incorporation into alginate hydrogel microcapsules can not only promote cell attachment and spreading as discussed above, but also upregulate osteogenic differentiation for encapsulated human MSCs (Fig. 7(C)) 157, 188 . Gelatin modification of alginate microcapsules can enhance adipogenic differentiation for human adipose-derived stem cells (ADSCs) 189 .…”

Section: Hydrogel Microencapsulation For 3d Cell Culturementioning

confidence: 89%

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

et al. 2017

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Hydrogel microcapsules provide miniaturized and biocompatible niches for three-dimensional (3D) in vitro cell culture. They can be easily generated by droplet-based microfluidics with tunable size, morphology, and biochemical properties. Therefore, microfluidic generation and manipulation of cell-laden microcapsules can be used for 3D cell culture to mimic the in vivo environment towards applications in tissue engineering and high throughput drug screening. In this review of recent advances mainly since 2010, we will first introduce general characteristics of droplet-based microfluidic devices for cell encapsulation with an emphasis on the fluid dynamics of droplet breakup and internal mixing as they directly influence microcapsule’s size and structure. We will then discuss two on-chip manipulation strategies: sorting and extraction from oil into aqueous phase, which can be integrated into droplet-based microfluidics and significantly improve the qualities of cell-laden hydrogel microcapsules. Finally, we will review various applications of hydrogel microencapsulation for 3D in vitro culture on cell growth and proliferation, stem cell differentiation, tissue development, and co-culture of different types of cells.

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Section: Hydrogel Microencapsulation For 3d Cell Culturementioning

confidence: 89%

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

et al. 2017

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“…Due to the increase in nucleophilicity of the thiol group when it is deprotonated, pH is a factor influencing the time of reaction so it can be reduced to just some minutes in acrylates when the crosslinking is conducted under basic conditions [78,206]. Higher concentrations and matched stoichiometric ratios of reactive groups can be employed to increase the rate at which the reactions are completed [47,207]. Amines are also employed in these reactions, but owing to their lower reactivity versus that of the thiol groups, the times of reaction usually range in the tens of minutes for both acrylate and vinyl sulfone groups [208,209].…”

Section: Chemistries For Arresting Phase Separationmentioning

confidence: 99%

“…Polysaccharides such as the anionic functional hyaluronic acid (HA), the cationic functional chitosan, and the hydroxyl functional dextran are macromolecules used in hydrogels due to their capability of interacting with structural proteins and growth factors, biodegradability [33][34][35][36][37][38], biocompatibility [39][40][41][42], and the versatility with which their surface can be modified due to their functionalities [43][44][45][46]. Among all the variety of methods to crosslink these materials so they behave as homogeneous hydrogels, Michael-type addition and photocrosslinking reactions have emerged as leading approaches [47,48].…”

Section: Introductionmentioning

confidence: 99%

Methods for producing microstructured hydrogels for targeted applications in biology

Garcia

Kiick

2019

Acta Biomaterialia

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Hydrogels have been broadly studied for applications in clinically motivated fields such as tissue regeneration, drug delivery, and wound healing, as well as in a wide variety of consumer and industry uses. While the control of mechanical properties and network structures are important in all of these applications, for regenerative medicine applications in particular, matching the chemical, topographical and mechanical properties for the target use/tissue is critical. There have been multiple alternatives developed for fabricating materials with microstructures with goals of controlling the spatial location, phenotypic evolution, and signaling of cells. The commonly employed polymers such as poly(ethylene glycol) (PEG), polypeptides, and polysaccharides (as well as others) can be processed by various methods in order to control material heterogeneity and microscale structures. We review here the more commonly used polymers, chemistries, and methods for generating microstructures in biomaterials, highlighting the range of possible morphologies that can be produced, and the limitations of each method. With a focus in liquidliquid phase separation, methods and chemistries well suited for stabilizing the interface and arresting the phase separation are covered. As the microstructures can affect cell behavior, examples of such effects are reviewed as well.

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“…Previously reported BSA gelation strategies included crosslinking of BSA with polymers, thiol–ene click chemistry, and glutaraldehyde crosslinking . The current strategy for BSA gelation is advantageous with respect to these earlier methods because no prior modification of BSA is required.…”

Section: Introductionmentioning

confidence: 99%

Protein Biophosphors: Biodegradable, Multifunctional, Protein‐Based Hydrogel for White Emission, Sensing, and pH Detection

Benson

Ghimire

Pattammattel

et al. 2017

Adv Funct Materials

108

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A highly efficient, multifunctional, bioderived white-emitting hydrogel (biophosphor) consisting of crosslinked bovine serum albumin and three fluorescent dyes, Coumarin 460, fluorescein, and 5(6)-carboxy-x-rhodamine, is reported here. White emission is obtained upon excitation of the biophosphor at 365 nm with appropriate mole ratios of the above dyes. The CIE 1931 chromaticity coordinates of white emission with 365 nm excitation are (0.36, 0.37), and the correlated color temperature is 5300 K. Multifunctional nature of the biophosphor is also demonstrated. A UV-light-emitting-diode (361 nm) coated with this biophosphor, for example, indicates white emission (CIE 0.28, 0.31) with a half-life of 106 (±5) h. The white emission is also highly sensitive to pH over a broad range (pH 1-11). Incorporation of glucose oxidase and peroxidase in the biophosphor allows for the detection of glucose over a physiologically relevant range of 1.8-288 mg dL −1 . This is a unique, advanced biophosphor with LED and sensing applications, and it is the first example of a multifunctional, proteinaceous white emitter. molar absorptivity and quantum efficiency of emissive components is a significant challenge for systems that combine direct emission and sensitized emission via Förster resonance energy transfer (FRET) to produce white light. [6,7] Hydrogels are hydrophilic polymer networks [8] and are emerging as versatile new matrices for high-efficiency generation of white light using intermolecular energy transfer processes. The gel matrix improves energy transfer efficiency by rigidifying the orientation of the donor (D)-acceptor (A) pairs [9] and preventing their aggregation, which can lead to quenching. [10] Despite the advantageous properties of white-emitting hydrogels, implementation in a functional device and photostability were not systematically studied, and biodegradability has not been demonstrated. Additionally, white-emitting proteinbased hydrogels are not known other than a report of a gelatin hydrogel with chromaticity coordinates [0.26, 0.33], far from being coordinates of pure white emission [0.33, 0.33]. [2] To the best of our knowledge, there are no reports of a multifunctional, nontoxic, biodegradable, white-emitting protein hydrogel.BSA is inexpensive and readily available as a waste product of the meat industry. BSA has a large number of primary amines (59 lysine) and carboxylic acids (99 aspartic acid/glutamic acid), [11] which can be crosslinked under controlled conditions by carbodiimide chemistry to form a network of amide bonds without disrupting the intricate secondary structure of the protein. [12,13] The protein's secondary structure plays an important role for dye binding at the intended site and for enzyme activity retention, when enzymes are incorporated in the matrix for sensing or catalytic applications. We envisioned that this molecular network of BSA would result in a water-rich hydrogel with discrete sites for dye binding which would be suitable for the construction of a white-emitting gel.Previou...

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A Universal and Facile Approach for the Formation of a Protein Hydrogel for 3D Cell Encapsulation

Cited by 23 publications

References 37 publications

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Methods for producing microstructured hydrogels for targeted applications in biology

Protein Biophosphors: Biodegradable, Multifunctional, Protein‐Based Hydrogel for White Emission, Sensing, and pH Detection

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