A universal boronate affinity capture-antibody-independent lateral flow immunoassay for point-of-care glycoprotein detection

Post-Assay Chemical Enhancement for Highly Sensitive Lateral Flow Immunoassays: A Critical Review

“…As the binding between nanoparticles is polyvalent (i.e., multiple molecules crosslink nanoparticles), moderate/low affine bindings can be used, as the high stability (i.e., no dissociation of nanoparticles aggregates) will be determined by high avidity [ 117 ]. Post-assay crosslinking can be driven by boronate affinity (phenylboronic acid and its derivatives binding with cis-diol-containing molecules such as glycoproteins, glycans) [ 118 ], hybridization of nucleic acids [ 119 ], vancomycin-D-alanyl-D-alanyl fragment in Gram-positive bacterial cell wall [ 120 ], barnase–barstar [ 121 ], small molecule–protein [ 122 ] and other types of binding.…”

Phenylboronic Acid-Modified Membrane-Like Magnetic Quantum Dots Enable the Ultrasensitive and Broad-Spectrum Detection of Viruses by Lateral Flow Immunoassay

“…coli], Staphylococcus aureus [S. aureus], and Klebsiella pneumoniae) have been developed with good stability and selectivity in complex samples. − Many main structural proteins (e.g., envelope protein and spike protein [SP]) of viruses are essentially GPs and highly immunogenic. − Inspired by this concept, herein, we first introduced phenylboronic acid into the LFIA system as the universal recognition molecule of the nanoprobe to achieve efficient and broad-spectrum detection of viral GPs in a rapid manner. To solve the problem of insufficient detection ability of common LFIA technologies, we also proposed a two-dimensional (2D) membrane-like magnetic fluorescent probe (GF@DQD) and demonstrated its superior performance in multiplex LFIA, which can support the ultrasensitive and simultaneous detection of multiple viruses.…”